Amino acid replacement: K1482term.
A9238464T
K1483term | mxc-PA; K1483term | mxc-PB
K1482term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Hemizygous larvae have overgrown lymph glands. The lymph gland lobes show increased mitotic activity compared to wild type. The concentration of circulating hemocytes in third instar larvae is significantly increased compared to controls and the fraction of circulating hemocytes that are undergoing cell division is also increased. The larvae show a significant increase in a blood cell type ("podocytes") with large pseudopod-like extensions compared to controls. These cells appear pear-shaped or spindle-shaped. Mutant larvae contain an increased fraction of lamellocytes (2.4%) compared to controls (0.2%). There is hardly any increase in cell death in the lymph glands of mutant larvae compared to controls. 70% of hemizygous lymph glands transplanted into wild-type females show an increase in size 5 days after transplantation, in contrast to wild-type lymph gland transplants, which do not show growth.
Transformation of second to first leg. Transformation of third to second leg.
10 to 35% of hemizygous males show loss of claws and have extra sex comb teeth on the meso- and metathoracic legs. A few individuals have small eyes.
Most homozygotes die as pharate adults. Lethal in combination with mxcSO. mxcG9/mxcG43 females have fewer egg chambers than wild-type flies, but are fully fertile and fecund. mxcM1/mxcG43 females do not lay eggs. Some egg chambers contain the normal number of nurse cells, but oogenesis appears arrested before stage 7-8. Egg chambers degenerate in older flies. Homozygous and mxcG46/mxcG43 females have rudimentary gonads and do not lay eggs. The sheaths of the somatic cells that organise the ovarioles are still present and all structures derived from genital imaginal disc appear similar to wild-type. Hemizygous males have short, thin, non-coiling, empty testes, and paragonia are very reduced compared to wild-type.
mxcG43 has mesothoracic leg phenotype, enhanceable by dom14/dom[+]
mxcG43 has metathoracic leg phenotype, enhanceable by dom14/dom[+]
mxcG43 has embryonic/larval hemocyte phenotype, non-enhanceable by Pcunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-enhanceable by Pclunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-enhanceable by Pscunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-enhanceable by Scmunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-enhanceable by escunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-enhanceable by morunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-enhanceable by trxunspecified
mxcG43 has embryonic/larval hemocyte phenotype, suppressible by Tlr3/Tlrv1
mxcG43 has embryonic/larval hemocyte phenotype, suppressible by brm[+]/brm2
mxcG43 has embryonic/larval hemocyte phenotype, suppressible by tub2/tub2
mxcG43 has lamellocyte phenotype, non-suppressible by Tlr3/Tlrv1
mxcG43 has lamellocyte phenotype, non-suppressible by tub2/tub2
mxcG43 has embryonic/larval plasmatocyte phenotype, non-suppressible by Tlr3/Tlrv1
mxcG43 has embryonic/larval plasmatocyte phenotype, non-suppressible by tub2/tub2
mxcG43 has embryonic/larval hemocyte phenotype, non-suppressible by Pcunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-suppressible by Pclunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-suppressible by Pscunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-suppressible by Scmunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-suppressible by Stat92E[+]/Stat92E06346
mxcG43 has embryonic/larval hemocyte phenotype, non-suppressible by escunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-suppressible by morunspecified
mxcG43 has embryonic/larval hemocyte phenotype, non-suppressible by trxunspecified
cact7, mxcG43 has embryonic/larval lymph gland phenotype
Dfd1, mxcG43 has adult head phenotype
The increased concentration of circulating hemocytes seen in mxcG43 larvae is suppressed by tub2/tub2 or Tlrv1/Tlr3, but the numbers of podocytes and lamellocytes are still increased in the double mutants compared to wild type. The increased concentration of circulating hemocytes seen in mxcG43 larvae is not suppressed by Stat92E06346/+. The concentration of circulating hemocytes in mxcG43/Y ; cact7/cact7 larvae is less than the number seen in either single mutant. The lymph glands of the double mutant larvae are very fragile, show severe overgrowth and contain high numbers of differentiated blood cells. There is intense mitotic activity in these glands. There is no increase in apoptotic cell death in these glands. The concentration of circulating hemocytes in mxcG43/Y ; Tl8/+ larvae is less than the number seen in either single mutant. mxcG43 hopTum/Y lymph glands contain differentiated hemocytes. The increased concentration of circulating hemocytes seen in mxcG43 larvae is suppressed by brm2/+.
The alleles of mxc form a series. From weakest phenotype to strongest phenotype: mxcG9 < mxcM1 < mxcG46 < mxcG43 < mxcSO < mxc1 < mxcG48.
Germline clonal analysis indicates that mxc+ function is required in the female germline.