Expression of a full length Acp26Aa cDNA included within a 0.7kb EcoRI fragment is governed by UAS regulatory sequences.
When Acp26AaScer\UAS.cPa is driven by Scer\GAL4hs.PB, in virgin females the release of oocytes fro the ovary is stimulated. The number of eggs in the reproductive tract is elevated. By 18h after heat shock, 67% of mutant females have an egg in the reproductive tract (as opposed to 8.3% in controls); most eggs are in the lateral oviducts. By 24h after heat shock, 17% of mutant females have more than one egg in their oviducts, and 12% had released eggs simultaneously into both lateral oviducts, a phenotype not seen in controls. No significant difference is found between the number of eggs in the reproductive tracts of mutant females at 18, 24, and 39h after heat shock. 18h after heat shock, 18% of mutant and control females deposit eggs. 24h heat shock however, 37% of mutant females desposit 4.2+-1.2 eggs per female. The number of eggs deposited by mutant females increases with time after heat shock, however, the average number of eggs deposited is very low compared to wild-type.
Expression of Acp26AaScer\UAS.cPa under the control of Scer\GAL4hs.PB, using heat shock, does not affect viability, when a single heat shock is applied during the L1 (day 1), L2 (day 2), L3 (day 3 or 4) or pupal stage (day 5, 6, 7 or 9).