FB2024_03 , released June 25, 2024
Allele: Dmel\cortQW55
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General Information
Symbol
Dmel\cortQW55
Species
D. melanogaster
Name
FlyBase ID
FBal0049392
Feature type
allele
Associated gene
Dmel\cort
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Lieberfarb et al., 1996, Page and Orr-Weaver, 1996, Schupbach and Wieschaus, 1989)
Progenitor genotype
Cytology
Description

Nucleotide substitution: A993G. Amino acid replacement: Y303C.

(Chu et al., 2001)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:6,675,461..6,675,461 [-]
Nucleotide change:

A6675461G

Reported nucleotide change:

A993G

Amino acid change:

Y303C | cort-PA; Y303C | cort-PB

Reported amino acid change:

Y303C

(Chu et al., 2001)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      0-2 hours eggs derived from cortQW55/cortRH65 females contain two spindles near the anterior cortex of the egg. Both of the spindles contain a single X-chromosome signal, indicating arrest in metaphase II.

      (Swan and Schupbach, 2007)

      A small percentage of embryos laid by grauRM61/Df(2R)Pu-D17 or grauQF31/Df(2R)Pu-D17 females develop into adult flies.

      (Chen et al., 2000)

      Transmission rate of Dp(1;f)J21A through females to progeny is 28%, cort mutation has no effect on transmission.

      (Cook et al., 1997)

      cort embryos do not develop to the cellular blastoderm stage. Embryos exhibit a complete absence of microtubule containing spindles, embryos do not initiate mitotic divisions. Oocyte meiosis is initiated but is arrested before completion. Sperm tails are present in embryos indicating a defect in oocyte fertilisation. Df(2L)cact-255rv64/cortQW55 embryos develop at 18oC but fail to develop at 29oC. A shift to the restrictive temperature during any stage of embryogenesis arrests embryonic development.

      (Lieberfarb et al., 1996)

      Females lay eggs that arrest in meiosis II, the earliest defect is in anaphase I. Premature sister chromatid separation does not rescue the meiotic defects, as shown in mei-S3321 double mutants. Male meiosis and mitosis are normal.

      (Page and Orr-Weaver, 1996)

      Eggs derived from homozygous females initiate development and cytoplasmic clearing occurs in a narrow zone around the egg periphery (in wild-type embryos this process is coupled to the arrival of the nuclei at the periphery). The eggs do not seem to develop beyond this stage; pole cells are not formed and cellularisation does not occur. However, about two hours after cytoplasmic clearing, the egg periphery starts to show local contractions, in what might be an attempt at gastrulation. These contractions eventually lead to eggs which typically have one or two condensed yolk balls surrounded by more transparent cytoplasm.

      (Schupbach and Wieschaus, 1989)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      cortQW55/cort[+] is an enhancer of lethal | maternal effect phenotype of grauRM61/Df(2R)Pu-D17

      (Chen et al., 2000)

      cortQW55/cort[+] is an enhancer of lethal | maternal effect phenotype of Df(2R)Pu-D17/grauQF31

      (Chen et al., 2000)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      74% of eggs derived from fzy6/fzy7 ; cortQW55/cortRH65 females raised at 29oC contain two spindles, each with a single X-chromosome signal, indicating arrest in metaphase of meiosis II. The remaining 26% of eggs contain only a single spindle containing two X-chromosome signals, indicating an arrest in meiosis I.

      (Swan and Schupbach, 2007)

      The small percentage of developing embryos laid by grauRM61/Df(2R)Pu-D17 or grauQF31/Df(2R)Pu-D17 females is abolished if the females are also heterozygous for cortQW55.

      (Chen et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      QH
      cort1
      cortQW55
      (Garrido et al., 2020, Pesin and Orr-Weaver, 2007, Sackton et al., 2007, Swan and Schupbach, 2007, Christensen and Cook, 2006.8.29, Christensen and Cook, 2006.12.5)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0001769
      References (17)