FlyBase Allele Report: Dmel\ksr[S-638]

General Information

Symbol

Dmel\ksr^S-638

Species

D. melanogaster

Name

FlyBase ID

FBal0049701

Feature type

allele

Associated gene

Dmel\ksr

Associated Insertion(s)

Carried in Construct

Also Known As

ksr^S638

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Therrien et al., 1995)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: A696V. Amino acid replacement: A703T. A703 is a highly conserved residue within the kinase II subdomain II.

(Therrien et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:5,481,551..5,481,551 [-]

Nucleotide change:

C5481551T

Amino acid change:

A696V | ksr-PA; A696V | ksr-PB; A696V | ksr-PC; A696V | ksr-PD

Reported amino acid change:

A696V

Comment:

One of two nucleotide substitutions in the ksr coding region in the allele.

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Therrien et al., 1995)

point_mutation

3R:5,481,531..5,481,531 [-]

Nucleotide change:

G5481531A

Amino acid change:

A703T | ksr-PA; A703T | ksr-PB; A703T | ksr-PC; A703T | ksr-PD

Reported amino acid change:

A703T

Comment:

One of two nucleotide substitutions in the ksr coding region in the allele. It falls within a highly conserved residue within the kinase subdomain II.

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Therrien et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Therrien et al., 1995)

Phenotype Manifest In

abdominal segment 8 & cephalopharyngeal skeleton | germ-line clone

(Therrien et al., 1995)

abdominal segment 9 & cephalopharyngeal skeleton | germ-line clone

(Therrien et al., 1995)

abdominal segment 10 & cephalopharyngeal skeleton | germ-line clone

(Therrien et al., 1995)

abdominal segment 11 & cephalopharyngeal skeleton | germ-line clone

(Therrien et al., 1995)

dorsal air sac primordium | somatic clone

(Cabernard and Affolter, 2005)

embryonic/larval visceral muscle cell | embryonic stage | maternal effect

(Wolfstetter et al., 2017)

embryonic/larval visceral muscle cell | embryonic stage | maternal effect (with ksr^S-627)

(Wolfstetter et al., 2017)

terminalia | germline clone

(Therrien et al., 1995)

Detailed Description

Statement

Reference

ksr^S-638 embryos (as well as ksr^S-638/ksr^S-627 that show a stronger phenotype) derived from mothers bearing ksr^S-638 mutant germline clones and thus lacking both maternal and zygotic ksr do not show any loss of muscle founder cells at late embryonic stages and only display rather mild visceral muscle phenotypes such as myotube stretching defects at stage 13/14 and incomplete midgut constriction at the end of embryogenesis.

(Wolfstetter et al., 2017)

ksr^S-638 homozygous clones in the dorsal air sac primordium grow more slowly than wild-type clones and never contribute to the tip of the primordium.

(Cabernard and Affolter, 2005)

Clonal analysis fails to produce clones of ksr^S-638 suggesting ksr is required for cell proliferation or survival. Embryos derived from germline clones reveal severe terminal defects. They are missing posterior structures beyond the seventh abdominal segment and have collapsed head skeleton. Embryos derived from germline clones of ksr^S-638 reveal severe terminal defects.

(Therrien et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ksr^S-638 is a suppressor of visible phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^dpp.blk1

(Ishimaru et al., 1999)

ksr^S-638 is a suppressor of visible phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

ksr^S-638 has terminalia | germline clone phenotype, suppressible by Raf::tor^13D.hs.sev

(Therrien et al., 1995)

Enhancer of

Statement

Reference

ksr[+]/ksr^S-638 is an enhancer of phenotype of csw^lf

(Firth et al., 2000)

Suppressor of

Statement

Reference

ksr^S-638 is a suppressor of wing vein L3 phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^dpp.blk1

(Ishimaru et al., 1999)

ksr^S-638 is a suppressor of eye phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

ksr^S-638 is a suppressor of eye phenotype of Ras85D^V12.sev

(Therrien et al., 1995)

ksr^S-638 is a suppressor of eye phenotype of Ras85D::Src64B^{V12.ΔCAAX.sev}

(Therrien et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Dominantly suppresses the eye phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^GMR.PF. Completely dominantly suppresses the wing vein phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^dpp.blk1.

(Ishimaru et al., 1999)

The terminal defect phenotype seen in germline clones of ksr^S-638 can be partially rescued by the injection of mRNA generated from phl::tor^13D.hs.sev. Dominantly suppresses the rough eye phenotype of Ras85D^V12.sev. Suppresses the rough eye phenotype of Ras85D::Src64B^{V12.ΔCAAX.sev}. The terminal defects of embryos derived from homozygous ksr^S-638 germline clones can be partially rescued by the injection of mRNA generated from phl::tor^13D.hs.sev.

(Therrien et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ksr^S-638

(Wolfstetter et al., 2017, Cabernard and Affolter, 2005)

ksr^S638

(Firth et al., 2000, Ishimaru et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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