FlyBase Allele Report: Dmel\HDAC1[5-5]

General Information

Symbol

Dmel\HDAC1^5-5

Species

D. melanogaster

Name

FlyBase ID

FBal0049831

Feature type

allele

Associated gene

Dmel\HDAC1

Associated Insertion(s)

Carried in Construct

Also Known As

Rpd3^m5-5, Hdac1^M5-5

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Gepner et al., 1996)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q109term.

(Zhu et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:4,627,779..4,627,779 [+]

Nucleotide change:

C4627779T

Amino acid change:

Q109term | HDAC1-PA

Reported amino acid change:

Q109term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zhu et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Pallos et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Aggarwal and Calvi, 2004, Aggarwal and Calvi, 2004)

lethal | recessive

(Gepner et al., 1996)

lethal - all die before end of second instar larval stage (with HDAC1^3-10)

(Zhu et al., 2008)

some die during second instar larval stage (with HDAC1^3-10)

(Zhu et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Rpd3^5-5 heterozygotes exhibit an extension in lifespan from a median of 49 to 56 days, compared to control lines.

(Pallos et al., 2008)

Rpd3^5-5 homozygous and Rpd3^5-5/Rpd3^3-10 transheterozygous mutants fail to survive beyond the end of the second instar larval stage.

(Zhu et al., 2008)

When homozygous mutant clones are made in the follicle cells of stage 10B ovaries, the mutant cells have nucleus wide hyper-acetylation. In clones of five or fewer cells, 20% appear to have undergone an extra genome reduplication rather than the developmental delay in replication that normally occurs before stage 10B.

(Aggarwal and Calvi, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

HDAC1^5-5, ttk^rM730 has visible phenotype, enhanceable by Mi-2⁴/Mi-2[+]

(Murawsky et al., 2001)

Enhancer of

Statement

Reference

HDAC1^5-5/Rpd3[+] is an enhancer of visible | dominant phenotype of Snr1^E1

(Zraly et al., 2004)

Suppressor of

Statement

Reference

HDAC1^5-5/Rpd3[+] is a suppressor of increased mortality phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Pallos et al., 2008)

HDAC1^5-5/Rpd3[+] is a suppressor of abnormal neuroanatomy phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Pallos et al., 2008)

NOT Suppressor of

Statement

Reference

HDAC1^5-5/Rpd3[+] is a non-suppressor of short lived | calorie restriction conditional phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Pallos et al., 2008)

Other

Statement

Reference

HDAC1^5-5, ttk^rM730 has visible phenotype

(Murawsky et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

HDAC1^5-5, ttk^rM730 has wing sensillum | ectopic phenotype, enhanceable by Mi-2⁴/Mi-2[+]

(Murawsky et al., 2001)

Enhancer of

Statement

Reference

HDAC1^5-5/Rpd3[+] is an enhancer of wing vein | ectopic phenotype of Snr1^E1

(Zraly et al., 2004)

HDAC1^5-5/Rpd3[+] is an enhancer of wing phenotype of Snr1^E1

(Zraly et al., 2004)

Suppressor of

Statement

Reference

HDAC1^5-5/Rpd3[+] is a suppressor of photoreceptor neuron phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Pallos et al., 2008)

HDAC1^5-5/Rpd3[+] is a suppressor of rhabdomere phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Pallos et al., 2008)

Other

Statement

Reference

HDAC1^5-5, ttk^rM730 has wing sensillum | ectopic phenotype

(Murawsky et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for Rpd3^5-5 has little effect on In(1)w^m4 eye colour variegation.

(Zhu et al., 2008)

At 29^oC, Rpd3^5-5/+ enhances the ectopic wing vein phenotype of Snr1^E1/+; 8% of flies have ectopic veins emanating from the posterior crossvein and posterior to the L5 longitudinal vein of the wing.

(Zraly et al., 2004)

ttk^rM730 Rpd3^5-5 double mutants have ectopic sensory bristles on the wing veins.

(Murawsky et al., 2001)

Xenogenetic Interactions

Statement

Reference

A heterozygous Rpd3^5-5 background markedly increases the survival rate of flies neuronally-expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} flies (from <10% to nearer 35% survival to eclosion). In addition, the level of neurodegeneration, as measured by the ratio of rhabdomeres per ommatidia, is also lowered in a Rpd3^5-5 heterozygous background.

A Rpd3^5-5 heterozygous background does not affect the short lifespan found in flies expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^elav-C155.

(Pallos et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

HDAC1^5-5

Hdac1^M5-5

(Zraly et al., 2004)

Rpd3^5-5

(Debruhl et al., 2013)

Rpd3^m5-5

(Pallos et al., 2008, Aggarwal and Calvi, 2004, Aggarwal and Calvi, 2004)

hdac1^m5-5

(Zhu et al., 2008)

l(3)64Cc^5-5

(Gepner et al., 1996)

rpd3^m5-5

(Murawsky et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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