FlyBase Allele Report: Dmel\scrib[smi97B]

General Information

Symbol

Dmel\scrib^smi97B

Species

D. melanogaster

Name

FlyBase ID

FBal0050518

Feature type

allele

Associated gene

Dmel\scrib

Associated Insertion(s)

P{lArB}scrib^smi97B

Carried in Construct

Also Known As

smi97B

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Anholt et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{lArB}scrib^smi97B

Cytology

Description

P{lArB} insertion in the promoter region, 1084bp upstream of the transcription start site.

(Anholt et al., 2003)

P{lArB} insertion 1084bp upstream of the scrib open reading frame.

(Ganguly et al., 2003)

P{lArB} insertion 159bp upstream of the transcription initiation site.

(Ganguly et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{lArB}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:26,536,539..26,536,539 [+]

(Anholt et al., 2003, Ganguly et al., 2003, Ganguly et al., 2001, Fedorowicz et al., 1998, Anholt et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous adults show impaired olfactory ability; the avoidance response to benzaldehyde is reduced compared to wild-type controls. This reduction in avoidance response is sexually dimorphic; homozygous males are hyposmic while homozygous females are anosmic (even at high odorant concentrations). The avoidance response of heterozygous adults to benzaldehyde is indistinguishable from wild type. Homozygous larvae show defects in response to isoamylacetate and benzaldehyde compared to controls. Larval motility in the absence of odour cue is not significantly different between homozygous and wild-type larvae. scrib^smi97B/Df(3R)Tl-X adults show reduced avoidance responses to benzaldehyde compared to Df(3R)Tl-X/+ controls and females show significantly more smell impairment than males. scrib^smi97B/scrib¹ and scrib^smi97B/scrib⁶⁷³ adults show a significantly lower avoidance response to benzaldehyde compared to controls. scrib^smi97B/scrib^S042405b adult females show a significantly lower avoidance response to benzaldehyde compared to control females, while the response of scrib^smi97B/scrib^S042405b adult males is not significantly different from that of control males. The avoidance response to benzaldehyde of scrib^j7B3/scrib^smi97B adults is not significantly different from that of controls.

(Ganguly et al., 2003)

Olfaction defective as measured in a dipstick assay using 1% benzaldehyde.

(Fedorowicz et al., 1998)

Avoidance score to benzaldehyde overlaps the avoidance score to water. Shows general hyposmia: avoidance score to 2-n-propylpyrazine and 2-isobutylthiazole is also reduced.

(Anholt et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Cam⁰³⁹⁰⁹, scrib[+]/scrib^smi97B has abnormal smell perception | dominant | male phenotype

(Anholt et al., 2003)

Hk¹, scrib[+]/scrib^smi97B has abnormal smell perception | dominant | male phenotype

(Anholt et al., 2003)

scrib^smi97B, sgg¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

scrib^smi97B, trp¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

Cam⁰³⁹⁰⁹, scrib^smi97B has abnormal smell perception | dominant | male phenotype

(Anholt et al., 2003)

Hk¹, scrib^smi97B has abnormal smell perception | dominant | male phenotype

(Anholt et al., 2003)

fax^BG00833, scrib^smi97B has abnormal smell perception | dominant | male phenotype

(Anholt et al., 2003)

scrib[+]/scrib^smi97B, sgg¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

scrib[+]/scrib^smi97B, trp¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

fax^BG00833, scrib[+]/scrib^smi97B has abnormal smell perception | dominant | male phenotype

(Anholt et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

scrib^smi97B has phenotype, enhanceable by smi51A¹

(Fedorowicz et al., 1998)

Suppressed by

Statement

Reference

scrib^smi97B has phenotype, suppressible by Pino^smi21F

(Fedorowicz et al., 1998)

Enhancer of

Statement

Reference

scrib^smi97B is an enhancer of phenotype of smi51A¹

(Fedorowicz et al., 1998)

Suppressor of

Statement

Reference

scrib^smi97B is a suppressor of phenotype of Pino^smi21F

(Fedorowicz et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

scrib^j7B3

(Ganguly et al., 2003)

scrib^j7B3

(Ganguly et al., 2001)

Partially complements

scrib^S042405b

(Ganguly et al., 2003)

Fails to complement

scrib¹

(Ganguly et al., 2003)

scrib⁶⁷³

(Ganguly et al., 2003)

scrib⁶⁷³

(Ganguly et al., 2001)

Rescued by

scrib^smi97B is rescued by scrib^hs.PB

(Ganguly et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the P{lArB} element reverts the olfactory deficit seen in scrib^smi97B mutants.

(Ganguly et al., 2003)

External Crossreferences and Linkouts ( 8 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

scrib^smi97B

smi97B

(Anholt et al., 2003, Ganguly et al., 2003, Ganguly et al., 2001)

smi97B¹

(Fedorowicz et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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