EcoRI-partial BglII fragment (-939 to +3544, coordinates from FBrf0047664) of the ro genomic clone and the BglII-EcoRI fragment (+3544 to +4368) from the ro cDNA c4-2. Results in deletion of the second intron.
wild-type
ro+tC4 rescues rounspecified
Completely abolishes ro function.
Completely abolishes ro function.