FlyBase Allele Report: Dmel\csw[LE120]

General Information

Symbol

Dmel\csw^LE120

Species

D. melanogaster

Name

FlyBase ID

FBal0057683

Feature type

allele

Associated gene

Dmel\csw

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Perkins et al., 1996)

Mutagen

ethyl methanesulfonate

(Perkins et al., 1996)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | embryonic stage | germline clone | rescuable maternal effect

(Perkins et al., 1996)

lethal | pupal stage | recessive

(Perkins et al., 1996)

Phenotype Manifest In

arista

(Perkins et al., 1996)

interommatidial bristle

(Perkins et al., 1996)

muscle cell of ventral acute muscle 2 | precursor

(Johnson Hamlet and Perkins, 2001)

neurogenic region

(Perkins et al., 1996)

ommatidium

(Perkins et al., 1996)

tarsal segment

(Perkins et al., 1996)

unguis

(Perkins et al., 1996)

wing vein L5

(Perkins et al., 1996)

Detailed Description

Statement

Reference

csw^LE120/Y embryos derived from homozygous female germline clones show loss of VA2 muscle precursor cells (only 53% of hemisegments contain VA2 precursor cells).

(Johnson Hamlet and Perkins, 2001)

csw^LE120 germline clone mosaics cause maternal effect lethality. In mutant embryos ventral cells undergo cell fate changes to that of more lateral epidermal cell fates. Pupal lethality can be rescued by heat induced expression of P{hs-SHP-2} during larval and pupal stages but not by expression of P{hs-SHP-1}.

(Perkins et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

csw^LE120 has lethal | pupal stage phenotype, suppressible by Hsap\PTPN11^hs.PP

(Perkins et al., 1996)

NOT suppressed by

Statement

Reference

csw^LE120 has lethal | pupal stage phenotype, non-suppressible by Hsap\PTPN6^hs.PP

(Perkins et al., 1996)

Enhancer of

Statement

Reference

csw^LE120 is an enhancer of visible phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^T155

(Perkins et al., 1996)

NOT Enhancer of

Statement

Reference

csw^LE120 is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

csw^LE120 is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

csw^LE120 has muscle cell of ventral acute muscle 2 | precursor phenotype, suppressible by Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

NOT Enhancer of

Statement

Reference

csw^LE120 is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

csw^LE120 is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG nearly completely suppresses the loss of VA2 muscle precursor cells seen in csw^LE120/Y embryos derived from homozygous female germline clones (94% of hemisegments have VA2 cells).

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^sca-C253 mediated expression of Egfr^{DN.Scer\UAS.cBa} generates flies with reduced eyes and females carrying Scer\GAL4^T155 and Egfr^{DN.Scer\UAS.cBa} lay partially to completely ventralised eggs. These phenotypes are enhanced in a csw^LE120 mutant background.

(Perkins et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

csw^LE120 is rescued by csw^hs.PP

(Perkins et al., 1996)

Comments

Mutant phenotype can be rescued by csw^hs.PP. Fully zygotically rescued adult males are fertile and when crossed to heterozygous females produce both male and female progeny. These females generate eggs with fused dorsal appendages. Examination of rescued adults reveals consistent defects; absence of one or both aristae, lack of one or more of the distal-most leg segments, incomplete formation of the distal portions of wing vein L5, eyes have reduced numbers of disorganised ommatidia and interommatidial bristles.

(Perkins et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23875

csw^LE120 P{FRT(w^hs)}101/FM7a

Notes on Origin

Discoverer

Comments

Phenotypic series of csw mutant embryos derived from homozygous germline clones, classified according to cuticular phenotype: csw⁵ = csw^LE120 > csw¹ = csw^13-87 > csw^e13d.3 > csw⁷ = csw⁶.

(Perkins et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

csw^LE120

(Popodi et al., 2010-, Settle et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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