A mutated form of arm, in which Arm repeat 5 is deleted (amino acids 309-351 are absent), is expressed under the control of its own promoter. The protein is tagged with a Tag:MYC epitope at residue 784 in the Gly/Pro-rich region close to the C-terminus. arm 3' trailer sequences are also present. The protein produced shows normal levels of binding to α-Cat protein in a coimmunoprecipitation assay, but binding to shg protein is reduced compared to wild-type controls.
Many photoreceptors are rescued from death in ApcQ8 homozygotes carrying one copy of armS5.T:Hsap\MYC in an arm4/+ background.
Embryos exhibit severe segment polarity defects and holes in the reamining cuticle.
armS5.Tag:MYC partially rescues arm3
Partial rescue of adherens junction defect.
Used in a coimmunoprecipitation assay to study shg binding and analysed by SDS-PAGE to study protein phosphorylation and stability.