FB2024_03 , released June 25, 2024
Allele: Dmel\armS15.Tag:MYC
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General Information
Symbol
Dmel\armS15.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0057836
Feature type
allele
Associated gene
Dmel\arm
Associated Insertion(s)
Carried in Construct
P{arm.S15}
Key Links
Transgenic product class
inframe_deletion
(Orsulic and Peifer, 1996)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Orsulic and Peifer, 1996)
Mutagen
in vitro construct
(Ahmed et al., 1998, Orsulic and Peifer, 1996)
Progenitor genotype
armS2.Tag:MYC
(Orsulic and Peifer, 1996)
Carried in construct
P{arm.S15}
Cytology
Description

A mutated form of arm, in which Arm repeat 8 is deleted (amino acids 432-475 are absent), is expressed under the control of its own promoter. The protein is tagged with a Tag:MYC epitope at residue 784 in the Gly/Pro-rich region close to the C-terminus. arm 3' trailer sequences are also present. The protein produced shows normal levels of binding to α-Cat protein in a coimmunoprecipitation assay, but binding to shg protein is reduced compared to wild-type controls.

(Orsulic and Peifer, 1996)
Allele components
Component
Use(s)
Regulatory region(s)
arm
Encoded product / tool
arm
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Many photoreceptors are rescued from death in ApcQ8 homozygotes carrying one copy of armS15.T:Hsap\MYC in an arm4/+ background.

      (Ahmed et al., 1998)

      Embryos exhibit severe segment polarity defects and embryos secrete small bits of cuticle.

      (Orsulic and Peifer, 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      armS15.Tag:MYC fails to rescue arm3

      (Orsulic and Peifer, 1996)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Used in a coimmunoprecipitation assay to study shg binding and analysed by SDS-PAGE to study protein phosphorylation and stability.

      (Orsulic and Peifer, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      S15
      (Ahmed et al., 1998)
      armS15.T:Hsap\MYC
      armS15.T:Myc
      armS15.Tag:MYC
      armS15
      (Miller and Moon, 1996)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)