FB2024_03 , released June 25, 2024
Allele: Dmel\perBG.lacZ
Open Close
General Information
Symbol
Dmel\perBG.lacZ
Species
D. melanogaster
Name
FlyBase ID
FBal0060951
Feature type
allele
Associated gene
Dmel\per
Associated Insertion(s)
Carried in Construct
P{per-lacZ.BG}
Also Known As
BG
Key Links
Transgenic product class
inframe_deletion
(Dembinska et al., 1997)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Dembinska et al., 1997)
Mutagen
in vitro construct
(Dembinska et al., 1997, Stanewsky et al., 1997)
Progenitor genotype
Carried in construct
P{per-lacZ.BG}
Cytology
Description

A 9.8kb BamHI genomic fragment of per, containing 4.2kb 5' flanking sequences and part of the coding region (encoding the N-terminal 868 amino acids) is fused in frame to lacZ.

(Dembinska et al., 1997)
Allele components
Component
Use(s)
Regulatory region(s)
per
per_BG
Encoded product / tool
per
Tagged with
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      larval LN period neuron

      Comment: Expression was strong in 3-5 neurons at ZT23.

      (Kaneko et al., 1997)
      larval DN1 neuron
      (Kaneko et al., 1997)
      larval DN2 neuron
      (Kaneko et al., 1997)
      larval DN3 neuron

      Comment: Staining only seen in 5% of brain hemispheres studied.

      (Kaneko et al., 1997)
      prepupal stage -- pupal stage
      pupal LN period neuron
      (Kaneko et al., 1997)
      pharate adult stage
      pupal l-LNv Pdf neuron
      (Kaneko et al., 1997)
      pupal s-LNv neuron
      (Kaneko et al., 1997)
      pupal DN1 period neuron
      (Kaneko et al., 1997)
      pupal DN2 period neuron
      (Kaneko et al., 1997)
      pupal DN3 period neuron
      (Kaneko et al., 1997)
      LNd neuron
      (Kaneko et al., 1997)
      Additional Information
      Statement
      Reference

      Expression is first seen in 4-5hr old first instar larvae at ZT0, though DN2 neurons stain most readily at ZT12. During the first 40% (prepupal to pupal stage) of pupal development, expression is seen only in the 4-5 LNs, and generally not in the DNs except for a single weakly labeled cell near the region of the DN2 in 1 of 16 examined hemispheres at ZT0. At approximately 50% pupal development (pharate adult stage), expression begins in DN1s, DN2s and the l-LNvs. At approximately 60% of pupal development, expression begins in the LNd and the DN3 neurons. Apart from DN2s and DN3s, all pupal cells expressing perBG.T:EcollacZ show cycling of the LacZ staining with a phase similar to the larval neurons. Peak expression in the larval LNs and DN1s is at ZT23, and a trough at ZT6-12. Peak expression in the larval DN2s is at approximately ZT12 and a trough at ZT0.

      (Kaneko et al., 1997)
       
      Marker for
      Reflects expression of
      per
      (Kaneko et al., 1997)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      per01; perBG.T:Ecol\lacZ flies are arrhythmic, perBG.T:Ecol\lacZ is unable to provide substantial per gene biological activity.

      (Dembinska et al., 1997)

      The phase of the evening activity peak of flies carrying perBG.T:Ecol\lacZ in a per+ background is significantly different to that of wild-type controls, being earlier, when flies are kept under 12 hour light/12 hour dark cycling conditions. The free-running locomotor activity periods of flies carrying perBG.T:Ecol\lacZ kept under DD conditions are significantly shorter than wild-type controls, and only 70-80% of the flies are rhythmic under these conditions.

      (Stanewsky et al., 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      perBG.lacZ fails to rescue per01

      (Stanewsky et al., 1997)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      perBG.T:Ecol\lacZ
      perBG.T:lacZ
      perBG.lacZ
      Name Synonyms
      Secondary FlyBase IDs
        References (7)