FlyBase Allele Report: Dmel\ttk[88.GMR.Tag:MYC]

General Information

Symbol

Dmel\ttk^{88.GMR.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0082129

Feature type

allele

Associated gene

Dmel\ttk

Associated Insertion(s)

Carried in Construct

P{GMR-ttk88}

Also Known As

GMR-ttk88

Key Links

Transgenic product class

wild_type

(Tang et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Tang et al., 1997)

Mutagen

in vitro construct

(Tang et al., 1997)

Progenitor genotype

Carried in construct

P{GMR-ttk88}

Cytology

Description

GMR regulatory sequences drive expression the ttk TTK88 protein isoform, tagged at the C-terminal end with a Tag:MYC epitope.

(Tang et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

ttk

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | late third instar larval stage

(Shi and Noll, 2009)

visible

(Tang et al., 2001)

visible | dominant

(Tang et al., 1997)

Phenotype Manifest In

cone cell

(Shi and Noll, 2009)

eye

(Tang et al., 2001, Tang et al., 1997)

eye-antennal disc

(Tang et al., 1997)

eye disc

(Shi and Noll, 2009)

eye photoreceptor cell

(Shi and Noll, 2009, Tang et al., 1997)

ommatidium

(Tang et al., 2001)

photoreceptor neuron

(Bai et al., 2001)

Detailed Description

Statement

Reference

All adult ttk^{88.GMR.T:Hsap\MYC}/+ flies are devoid of photoreceptors. Cell death is elevated in late third instar eye discs for strong ttk^{88.GMR.T:Hsap\MYC} lines. 88% of ttk^{88.GMR.T:Hsap\MYC}/+ ommatidia lack 1-3 cone cells, while only 4% contain 1-2 supernumerary cone cells.

(Shi and Noll, 2009)

Photoreceptor cell determination is blocked.

(Bai et al., 2001)

Flies carrying ttk^{88.GMR.T:Hsap\MYC} have a rough eye phenotype.

(Tang et al., 2001)

Flies carrying ttk^{88.GMR.T:Hsap\MYC} have rough eyes that are devoid of photoreceptors. Third larval instar eye imaginal discs carrying ttk^{88.GMR.T:Hsap\MYC} show increased cell death compared to wild-type.

(Tang et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ttk^{88.GMR.Tag:MYC} has increased cell death | late third instar larval stage phenotype, suppressible by sv^GMR.PS

(Shi and Noll, 2009)

ttk^{88.GMR.Tag:MYC} has visible phenotype, suppressible by lilli^XS407

(Tang et al., 2001)

ttk^{88.GMR.Tag:MYC} has visible phenotype, suppressible by lilli^s35

(Tang et al., 2001)

ttk^{88.GMR.Tag:MYC} has visible phenotype, suppressible by lilli^XS575

(Tang et al., 2001)

Suppressor of

Statement

Reference

ttk^{88.GMR.Tag:MYC} is a suppressor of visible | dominant phenotype of phyl^GMR.PC

(Tang et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

ttk^{88.GMR.Tag:MYC} has cone cell | pupal stage | increased number phenotype, enhanceable by sv^GMR.PS

(Shi and Noll, 2009)

Suppressed by

Statement

Reference

ttk^{88.GMR.Tag:MYC} has ommatidium phenotype, suppressible by lilli^XS407

(Tang et al., 2001)

ttk^{88.GMR.Tag:MYC} has ommatidium phenotype, suppressible by lilli^XS575

(Tang et al., 2001)

ttk^{88.GMR.Tag:MYC} has ommatidium phenotype, suppressible by lilli^s35

(Tang et al., 2001)

NOT suppressed by

Statement

Reference

ttk^{88.GMR.Tag:MYC} has photoreceptor neuron phenotype, non-suppressible by ed^slH8/ed^1X5

(Bai et al., 2001)

Enhancer of

Statement

Reference

ttk^{88.GMR.Tag:MYC} is an enhancer of cone cell | pupal stage | increased number phenotype of sv^GMR.PS

(Shi and Noll, 2009)

Suppressor of

Statement

Reference

ttk^{88.GMR.Tag:MYC} is a suppressor of eye phenotype of phyl^GMR.PC

(Tang et al., 1997)

ttk^{88.GMR.Tag:MYC} is a suppressor of photoreceptor neuron phenotype of phyl^GMR.PC

(Tang et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Ectopic expression of both sv^GMR.PS and ttk^{88.GMR.T:Hsap\MYC} generates supernumerary cone cells in about 90% of the ommatidia of pupal eye discs, with a maximum of 11 cone cells observed in one ommatidium.

More than half of the ommatidia in sv^GMR.PS ttk^{88.GMR.T:Hsap\MYC} eyes have two or three supernumerary cone cells: 30% have three, 24% have two, and 14% have one supernumerary cone cell.

The addition of sv^GMR.PS results in less photoreceptor cell death in ttk^{88.GMR.T:Hsap\MYC} third instar eye discs.

(Shi and Noll, 2009)

Flies carrying both phyl^GMR.PC and ttk^{88.GMR.T:Hsap\MYC} have nearly wild-type eyes, with most of the ommatidia having close to the normal number of photoreceptor cells. sina²/sina³ flies carrying both phyl^GMR.PC and ttk^{88.GMR.T:Hsap\MYC} have very disrupted eyes, similar to those of flies carrying ttk^{88.GMR.T:Hsap\MYC} alone.

(Tang et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ttk^{88.GMR.T:Hsap\MYC}

ttk^88.GMR.T:Myc

ttk^{88.GMR.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close