FlyBase Allele Report: Dmel\Rap1[CD3]

General Information

Symbol

Dmel\Rap1^CD3

Species

D. melanogaster

Name

FlyBase ID

FBal0083270

Feature type

allele

Associated gene

Dmel\Rap1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Asha et al., 1999)

Mutagen

Progenitor genotype

Cytology

Description

4.5kb deletion that removes R coding region.

(Asha et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | germline clone | non-rescuable maternal effect

(Asha et al., 1999)

some die during embryonic stage | non-rescuable maternal effect

(Asha et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

Loss of maternal and zygotic R in R^CD3 germ-line clones disrupts ventral cuticle resulting in twi-positive mesoderm remaining on the surface after gastrulation. In some R^CD3 mutants the germband becomes twisted during gastrulation. Coupling between actomyosin constriction and cell shape change is disrupted in R^CD3 mutants. Balls of actin and myosin appear at the apical surface of mesodermal cells, and cell constriction halts prematurely, with myosin balls not contiguous with adherens junctions.

(Sawyer et al., 2009)

Mutant clones in the eye show no defect at early pupal stages. But in the resulting adult eyes scars indicate that mutant tissue does not survive to the adult stage. Remnants of rhabdomere are present in the degenerated tissue. Mutants derived from germline clones show (non-paternally-rescuable) embryonic lethality. Affected embryos show normal axis specification, including for the terminal system. Gastrulation is affected, for 50% of embryos the ventral furrow never closes, although the mesodermal precursors display the usual apical construction and membrane blebbing. At the time of ventral furrow formation nuclei of invaginating cells are found at a variety of levels, instead of in the more usual basal location. Dorsal migration of internalised mesoderm is less extensive than in wild type. Dorsal structures such as the dorsal vessel do not form. Tracheal development seems relatively unaffected. Pole cell migration is severely impaired. Only a small number of cells reach the gonad. Head involution frequently fails and dorsal closure fails 5-10% of the time. These defects are decreased by paternally rescuing R⁺. The cytoskeleton in gastrulating embryos seems relatively normal.

(Asha et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rap1^B3/Rap1^CD3 is an enhancer of increased mortality during development phenotype of PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

Rap1^B3/Rap1^CD3 is an enhancer of increased mortality during development phenotype of PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

NOT Enhancer of

Statement

Reference

R[+]/Rap1^CD3 is a non-enhancer of visible phenotype of CycE^JP

(Brumby et al., 2004)

NOT Suppressor of

Statement

Reference

R[+]/Rap1^CD3 is a non-suppressor of visible phenotype of CycE^JP

(Brumby et al., 2004)

Other

Statement

Reference

PDZ-GEF^C1/PDZ-GEF^k13720, Rap1^B3/Rap1^CD3 has abnormal cell migration | embryonic stage phenotype

(Boettner and Van Aelst, 2007)

PDZ-GEF^k13720/PDZ-GEF^C2, Rap1^B3/Rap1^CD3 has abnormal cell migration | embryonic stage phenotype

(Boettner and Van Aelst, 2007)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rap1^B3/Rap1^CD3 is an enhancer of embryo phenotype of PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

Rap1^B3/Rap1^CD3 is an enhancer of embryo phenotype of PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

NOT Enhancer of

Statement

Reference

R[+]/Rap1^CD3 is a non-enhancer of eye phenotype of CycE^JP

(Brumby et al., 2004)

NOT Suppressor of

Statement

Reference

R[+]/Rap1^CD3 is a non-suppressor of eye phenotype of CycE^JP

(Brumby et al., 2004)

Other

Statement

Reference

PDZ-GEF^C1/PDZ-GEF^k13720, Rap1^B3/Rap1^CD3 has embryonic/first instar larval cuticle | embryonic stage phenotype

(Boettner and Van Aelst, 2007)

PDZ-GEF^C1/PDZ-GEF^k13720, Rap1^B3/Rap1^CD3 has ectoderm | embryonic stage phenotype

(Boettner and Van Aelst, 2007)

PDZ-GEF^k13720/PDZ-GEF^C2, Rap1^B3/Rap1^CD3 has embryonic/first instar larval cuticle | embryonic stage phenotype

(Boettner and Van Aelst, 2007)

PDZ-GEF^k13720/PDZ-GEF^C2, Rap1^B3/Rap1^CD3 has ectoderm | embryonic stage phenotype

(Boettner and Van Aelst, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

R^CD3

Rap1^1CD3

(Mishra et al., 2005)

Rap1^CD3

(Sawant et al., 2018, Baril et al., 2014, Wang et al., 2013, Sawyer et al., 2009, Boettner and Van Aelst, 2007, Brumby et al., 2004, Asha et al., 1999, Li et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close