FB2024_03 , released June 25, 2024
Allele: Dmel\Pka-R1BGD.UAS
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General Information
Symbol
Dmel\Pka-R1BGD.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0086778
Feature type
allele
Associated gene
Dmel\Pka-R1
Associated Insertion(s)
Carried in Construct
P{UAS-Pka-R1.BGD}
Key Links
Transgenic product class
missense_variant
(Kalderon, 1998.3.12)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kalderon, 1998.3.12)
Mutagen
in vitro construct
(Davis et al., 1998, Kalderon, 1998.3.12, Li et al., 1995)
Progenitor genotype
Carried in construct
P{UAS-Pka-R1.BGD}
Cytology
Description

UAS regulatory sequences drive expression of a mutant form of Pka-R1 that contains a mutation in a cAMP binding site (amino acid replacement G321D). This mutant regulatory subunit acts as an inhibitor of protein kinase A (PKA) activity; although the subunit can be activated in vitro by cAMP, it requires cAMP concentrations higher than those likely to be seen in vivo.

(Kalderon, 1998.3.12)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Pka-R1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      There is a significant increase in average miniature excitatory junctional current (mEJC) amplitude in larvae carrying Pka-R1BGD.Scer\UAS expressed under the control of Scer\GAL4hs.PB in a Df(2L)γ15 background even in the absence of heat shock, when compared to wild-type larvae. There is a further significant increase in average mEJC amplitude when the larvae are heat shocked.

      (Davis et al., 1998)

      Partial wing duplication, cuticular outgrowth, distally truncated tarsae with multiple ectopic bristle columns.

      (Li et al., 1995)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Pka-R1BGD.Scer\UAS
      Pka-R1BGD.UAS
      Pka-R1R.UAS
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0042699
      References (7)