FlyBase Allele Report: Dmel\Cdc27[L7123]

General Information

Symbol

Dmel\Cdc27^L7123

Species

D. melanogaster

Name

FlyBase ID

FBal0087027

Feature type

allele

Associated gene

Dmel\Cdc27

Associated Insertion(s)

P{lacW}Cdc27^L7123

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Cdc27^L7123

Cytology

Description

Insertion 532bp upstream of the ATG codon.

(Deak et al., 2003)

Insertion 519bp upstream of the initiating ATG codon.

(Huang and Raff, 2002)

The insertion is in the 5'UTR ofthe Cdc27 transcript.

(Deak, 1999.9.15)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:7,249,214..7,249,214 [+]

(Deak et al., 2003, Huang and Raff, 2002, Deak, 1999.9.15, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

aster & neuroblast

(Deak et al., 2003)

chaeta

(Huang and Raff, 2002)

chromatin & neuroblast

(Deak et al., 2003)

chromosome & neuroblast

(Deak et al., 2003)

chromosome | increased number

(Deak et al., 2003)

eye

(Huang and Raff, 2002)

mitotic metaphase & neuroblast

(Deak et al., 2003)

spindle & neuroblast

(Deak et al., 2003)

Detailed Description

Statement

Reference

In Cdc27^L7123 mutants, there is a high proportion of cells in a metaphase-like state (a ratio of 8.7 metaphase:anaphase compared to 2.8 in wild-type). Along with the low proportion of cells in anaphase, this suggests that Cdc27^L7123 cells arrest or delay in metaphase. The extent of chromosome condensation observed indicates that cells have been delayed in metaphase for a period of time. Cdc27^L7123 anaphase cells are frequently disorganised, with chromosomes lagging along the spindle. A low frequency (~3% of mitoses) of polyploid cells are also found in Cdc27^L7123 cells. In Cdc27^L7123 larval stage brains the sister chromosomes of at least one chromosome are found to be separated in 20% of all mitotic figures. 19% of Cdc27^L7123 mitotic cells are found to contain a 4N complement of chromosomes in which there are four sites of hybridization indicating that the sister chromosomes are separated. This is compared to a frequency of 0.4% in wild-type metaphase cells. In mitotic Cdc27^L7123 cells, the centromeric regions of the two pairs of major autosomes are present as a series of four separate pairs of dots, indicative of chromosome separation. Approximately 3.9% of Cdc27^L7123 cells contain a replicated tetraploid set of chromosomes. Chromatin in Cdc27^L7123 mitotic cells is distributed in a broad band. Cdc27^L7123 cells exhibit overcondensed chromosomes. Up to 16 separated kinetochores of sister chromosomes are observed in Cdc27^L7123 cells, similar to the phenotype observed in polo hypomorphs.

(Deak et al., 2003)

Homozygous flies occasionally survive to adulthood. These escapers have many eye and bristle defects, are invariably sterile and only live for a few days.

(Huang and Raff, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Cdc27[+]/Cdc27^L7123 is a non-suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Cdc27[+]/Cdc27^L7123 is a non-suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Cdc27[+]/Cdc27^L7123 is a non-suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The slight increase in lymph gland size seen in Zfrp8^SM206/+ larvae is dominantly enhanced by Cdc27^L7123.

(Minakhina et al., 2007)

Examination of the larval stage central nervous system of BubR1^unspecified Cdc27^L7123 double mutant shows cells arrested at metaphase at a comparable frequency to the Cdc27^L7123 mutant alone. Moreover, the high frequency of anaphases seen in the BubR1^unspecified mutant as a result of the malfunctioning checkpoint is replaced in the double mutant by an elevated frequency of metaphase-like figures typical of Cdc27^L7123. The appearance of mitotic figures in squashed preparations of BubR1^unspecified Cdc27^L7123 is indistinguishable from those seen in Cdc27^L7123 alone.