FlyBase Allele Report: Dmel\mof[2]

General Information

Symbol

Dmel\mof²

Species

D. melanogaster

Name

FlyBase ID

FBal0088802

Feature type

allele

Associated gene

Dmel\mof

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Truncated protein of 370 instead of 827 amino acids.

(Gu et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:5,875,642..5,875,642 [-]

Nucleotide change:

C5875642T

Amino acid change:

Q371term | mof-PA

Comment:

Site of nucleic acid change inferred from report that a truncated protein of 370 amino acids is made in the mutant.

(Gu et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT exacerbate Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Bodai et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | germline clone | male limited | maternal effect

(Copur et al., 2018)

Detailed Description

Statement

Reference

Zygotic-only mof² mutants (homozygotes) are male larval lethal and female semi-viable; female adults are short lived. Maternal-only mof² mutants are lethal. Maternal-zygotic mof² mutants are male embryonic lethal and female semi-lethal; all male embryos arrest development during or shortly after gastrulation with highly abnormal morphology such as irregular organization of epidermal tissues, aberrant invagination of the stomodaeum, and the hindgut, and failure to form a proper cephalic furrow.

Epidermal mof² clones in the mof^+t6.8 background are able to form normal-looking adult structures with sensory bristles.

(Copur et al., 2018)

mof²/Y hemizygous males are lethal

(Schunter et al., 2017)

Mutant males show full lethality, whereas the number of females reaching adulthood is reduced approximately 2-fold compared to wild type. Most mutant female adults are sterile. The average lifespan of mutant females is reduced to 8 days compared to 37 days in controls.

(Conrad et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

mof[+]/mof² is an enhancer of partially lethal - majority die | dominant | female phenotype of sc^sisB-1, sisA[+]/sisA¹

(Gladstein et al., 2010)

NOT Enhancer of

Statement

Reference

mof[+]/mof² is a non-enhancer of partially lethal - majority die phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Bodai et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

One paternally-derived copy of mof² reduces the viability of sc^sisB-1 sisA¹/+ females.

(Gladstein et al., 2010)

Xenogenetic Interactions

Statement

Reference

One copy of mof² does not enhance the reduction in eclosion rate seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155.

(Bodai et al., 2012)

Complementation and Rescue Data

Rescued by

mof² is rescued by mof^UAS.Tag:FLAG/Scer\GAL4^arm.PU

(Schunter et al., 2017)

mof² is rescued by mof^{2KN.UAS.Tag:FLAG}/Scer\GAL4^arm.PU

(Schunter et al., 2017)

mof² is rescued by mof^{7KN.UAS.Tag:FLAG}/Scer\GAL4^arm.PU

(Schunter et al., 2017)

mof² is rescued by mof^{9KN.UAS.Tag:FLAG}/Scer\GAL4^arm.PU

(Schunter et al., 2017)

mof² is rescued by Scer\GAL4^arm.PU/mof^{3KC.UAS.Tag:FLAG}

(Schunter et al., 2017)

mof² is rescued by mof^UAS.Tag:HA/Scer\GAL4^arm.PU

(Conrad et al., 2012)

Partially rescued by

mof² is partially rescued by mof^{9KC.UAS.Tag:FLAG}/Scer\GAL4^arm.PU

(Schunter et al., 2017)

mof² is partially rescued by mof^{Δ1.UAS.Tag:HA}/Scer\GAL4^arm.PU

(Conrad et al., 2012)

mof² is partially rescued by mof^{Δ2.UAS.Tag:HA}/Scer\GAL4^arm.PU

(Conrad et al., 2012)

mof² is partially rescued by mof^{Δ3.UAS.Tag:HA}/Scer\GAL4^arm.PU

(Conrad et al., 2012)

mof² is partially rescued by mof^{ΔN.UAS.Tag:HA}/Scer\GAL4^arm.PU

(Conrad et al., 2012)

Not rescued by

mof² is not rescued by mof^{ΔN.UAS.Tag:FLAG}/Scer\GAL4^arm.PU

(Schunter et al., 2017)

mof² is not rescued by mof^{Nt.UAS.Tag:FLAG}/Scer\GAL4^arm.PU

(Schunter et al., 2017)

mof² is not rescued by Scer\GAL4^arm.PU/mof^{ΔZn.UAS.Tag:HA}

(Conrad et al., 2012)

mof² is not rescued by mof^{Y416D.UAS.Tag:HA}/Scer\GAL4^arm.PU

(Conrad et al., 2012)

mof² is not rescued by mof^{W426G.UAS.Tag:HA}/Scer\GAL4^arm.PU

(Conrad et al., 2012)

Comments

The lethality of mof² hemizygous mutant males is strongly rescued by expression of any of the following:mof^{Scer\UAS.T:Zzzz\FLAG}, mof^{2KN.Scer\UAS.T:Zzzz\FLAG}, mof^{7KN.Scer\UAS.T:Zzzz\FLAG}, mof^{9KN.Scer\UAS.T:Zzzz\FLAG}, mof^{3KC.Scer\UAS.T:Zzzz\FLAG} or mof^{9KC.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^arm.PU and partially also by expression of mof^{9KC.Scer\UAS.T:Zzzz\FLAG}, whereas expression of either mof^{ΔN.Scer\UAS.T:Zzzz\FLAG} or mof^{Nt.Scer\UAS.T:Zzzz\FLAG} does not efficiently rescue survival of the male mutants as only few escapers emerge.

(Schunter et al., 2017)

Expression of mof^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PU completely rescues the lethality of mof² males.

Expression of either mof^{ΔZn.Scer\UAS.T:Ivir\HA1}, mof^{W426G.Scer\UAS.T:Ivir\HA1} or mof^{Y416D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PU fails to rescue the lethality of mof² males.

The lethality of mof² males is partially rescued by expression of either mof^{Δ1.Scer\UAS.T:Ivir\HA1} (49.6% rescue), mof^{Δ2.Scer\UAS.T:Ivir\HA1} (59.3% rescue), mof^{Δ3.Scer\UAS.T:Ivir\HA1} (42.5% rescue) or mof^{ΔN.Scer\UAS.T:Ivir\HA1} (11.2% rescue) under the control of Scer\GAL4^arm.PU.

(Conrad et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mof²

(Samata et al., 2020, Copur et al., 2018, Schunter et al., 2017, Chlamydas et al., 2016, Figueiredo et al., 2014, Bodai et al., 2012, Conrad et al., 2012, Gladstein et al., 2010, Gelbart et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (13)

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