tetO regulatory sequences plus the human cytomegalovirus virus 1E promoter drive expression of some copies of a Hsap\HMGA1 fragment from amino acids 38-107 containing H2 and H3 hooks in head to tail orientation. A SV40 polyadenylation signal is also present.
Tn10\tetRey.3.5.T:Hsim\VP16-mediated expression of Hsap\HMGIYMATH11.Tn10\tetO in In(1)wm4 flies results in no significant derepression of the w gene, no loss of the variegating phenotype.
Used in the tetracyclin (tet)-regulated transactivation system. Addition of tetracycline to the food completely silences Tn10\tetRey.3.5.T:Hsim\VP16-mediated expression of Hsap\HMGIYMATH11.Tn10\tetO.