Carries 2 deletions of ~1.5kb and ~1kb, separated by 953bp of wild-type EcR-B genomic sequence. A 7bp stretch of the P{lacW}EcRk04504 element remains.
Imprecise excision of the P{lacW} element, resulting in a 2.5kb deletion.
lethal (with Df(2R)ED1482)
adult brain (with EcRW53st)
gamma-lobe & neuron (with EcRW53st)
neuron & ventral nerve cord (with EcR99)
EcR31/EcR99 mutants (that are mutant for both EcR-B1 amd EcR-B2 isoforms) are developmentally arrested during the course of larval growth, although a few larval escapers develop into prepupae. At the larval stage, in the escapers, approximately 12 Crz-expressing ventral nerve cord neurons are present, while in wild-type they are absent.
Mutants that lack EcR-B1 (e.g. EcRQ50st/EcR31) exhibit no difference in Crz-expressing ventral nerve cord neuronal cell death compared to wild-type.
EcR31/EcR99 mutants, that lack EcR-B isoforms, exhibit significant differences to EcRB2.Scer\UAS or EcRB1.Scer\UAS rescued lines. Complete lack of programmed cell death is observed in 18% of the EcR-B mutants central nervous system, and partial cell death in the remaining 82% of specimens. Overall, approximately 75% of Crz-expressing ventral nerve cord neurons do not undergo programmed cell death in EcR-B mutants. By contrast, expression of EcRB1.Scer\UAS or EcRB2.Scer\UAS, under the control of Scer\GAL4Crz restores complete cell death in 73% or 87% of EcR31/EcR99 tissue, respectively, while four to eleven neurons are detectable in the remaining specimens.
Most EcR31/EcRM554fs animals fail to survive to the second larval instar stage and few mutant animals survive to the 3rd larval instar stage. 65-80% of larvae arrested prior to the second instar stage have both 1st and 2nd instar mouth hooks, while those arrested prior to the third instar stage have both 2nd and 3rd instar mouth hooks.
EcR31/EcR[+] is a non-enhancer of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4ppk.PG, par-1HMS00405
EcR31/EcR[+] is a non-enhancer of larval multidendritic class IV neuron | pupal stage phenotype of Scer\GAL4ppk.PG, par-1HMS00405
EcR31/EcR[+] is a non-enhancer of dendrite | pupal stage phenotype of Scer\GAL4ppk.PG, par-1HMS00405
The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1HMS00405 under the control of Scer\GAL4ppk.PG is not significantly modified by combination with a single copy of EcR31.
EcR99/EcR31 is rescued by EcRB1.UAS/Scer\GAL4Crz.PC
EcR99/EcR31 is rescued by Scer\GAL4Crz.PC/EcRB2.UAS
EcR31/EcRW53st is rescued by Scer\GAL4Tab2-201Y/EcRB1.UAS
EcR31/EcRW53st is rescued by Scer\GAL4Tab2-201Y/EcRB2.UAS
EcR99/EcR31 is partially rescued by Scer\GAL4FMRFa.PS/EcRA.UAS
EcR99/EcR31 is partially rescued by Scer\GAL4FMRFa.PS/EcRB2.UAS
EcR99/EcR31 is partially rescued by EcRB1.UAS/Scer\GAL4FMRFa.PS
EcR31/EcRW53st is not rescued by Scer\GAL4Tab2-201Y/EcRA.UAS
Expression of EcRB1.Scer\UAS or EcRB2.Scer\UAS, under the control of Scer\GAL4Crz restores complete cell death in 73% or 87% of EcR31/EcR99 tissue, respectively, while four to eleven neurons are detectable in the remaining specimens.