In combination with Tn10\tetRhs.T:Hsim\VP16, 30 minute heat shock at 6.5 hours of embryogenesis causes neither failure of head involution in early embryos nor defects on the dorsal surface of the head and disruptions of the prothoracic denticle belts.
Addition of tetracycline to the food completely silences Tn10\tetR-T:Hsim\VP16-mediated expression.