FlyBase Allele Report: Dmel\mira[AB78]

General Information

Symbol

Dmel\mira^AB78

Species

D. melanogaster

Name

FlyBase ID

FBal0092625

Feature type

allele

Associated gene

Dmel\mira

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Matsuzaki et al., 1998)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C1459T.

(Fuerstenberg et al., 1998)

Encodes a truncated protein containing the N-terminal 405 amino acids.

(Matsuzaki et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:19,933,458..19,933,458 [-]

Nucleotide change:

C19933458T

Amino acid change:

Q406term | mira-PA; Q406term | mira-PB; Q406term | mira-PC

Comment:

Premature stop codon gives rise to a 405aa protein; site of nucleic acid substitution inferred by FlyBase based on reported amino acid change

(Matsuzaki et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic ganglion mother cell

(Fuerstenberg et al., 1998)

embryonic neuroblast

(Fuerstenberg et al., 1998)

ventral neurectoderm

(Fuerstenberg et al., 1998)

Detailed Description

Statement

Reference

Neuroectodermal cells are irregularly sized and spaced in homozygous embryos, often having a "wavy" cell outline. Neuroblasts are elongated compared to spherical wild-type neuroblasts and ganglion mother cells are misshapen and appear to slide apically between neuroblasts rather than forming a distinct basal layer. Neuroblasts and ganglion mother cells in the neuroectoderm show a uniform cortical distribution of actin in the mutant embryos, as occurs in wild type.

(Fuerstenberg et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

mira[+]/mira^AB78 is an enhancer of egg chamber phenotype of cup²¹

(Piccioni et al., 2009)

mira[+]/mira^AB78 is an enhancer of egg chamber phenotype of cup¹⁵

(Piccioni et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The ovarian phenotype of cup¹⁵ and cup²¹ homozygous females is enhanced if they are also heterozygous for mira^AB78; a greater fraction of egg chambers arrest development between stages 3 and 5 in the double mutant ovaries compared to in the single mutant ovaries.

(Piccioni et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mir^AB78

(Fuerstenberg et al., 1998)

mira^AB78

(Piccioni et al., 2009, Lee et al., 2006)

miranda^AB78

(Fuerstenberg et al., 1998)

miranda^AB

(Fuerstenberg et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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