FlyBase Allele Report: Dmel\cnc[B.hs]

General Information

Symbol

Dmel\cnc^B.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0094556

Feature type

allele

Associated gene

Dmel\cnc

Associated Insertion(s)

Carried in Construct

P{hs-cnc.B}

Also Known As

HS-cncB

Key Links

Transgenic product class

wild_type

(McGinnis et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(McGinnis et al., 1998)

Mutagen

in vitro construct

(McGinnis et al., 1998)

Progenitor genotype

Carried in construct

P{hs-cnc.B}

Cytology

Description

A heat shock promoter drives expression of the cnc B isoform. The construct contains 390bp of 5' UTR and 1230bp of 3' UTR from exon A3.

(McGinnis et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

cnc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Veraksa et al., 2000)

lethal | heat sensitive

(McGinnis et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ubiquitous heat-shock induced expression of cnc^B.hs 6 and 8 hours of development is lethal for 99% of embryos, and most lack mouth hooks and other head structures.

(Veraksa et al., 2000)

Ectopic expression of cnc^B.hs at 4-10 hours of embryonic development results in lethality. Ectopic expression at 6-8 hours after egg laying results in a defective head phenotype; rudimentary mouth hooks, H-piece and cirri are formed. The anterior part of the lateralgraten are truncated. The dorsal bridge is abnormal or absent. The antennal sense organ, vertical plates and T-ribs develop normally. These head defects are produced at high penetrance (greater than 95%) by heat shocks between 4-10 hours of development. 10-70% of these embryos (depending on the stage of heat shock) show replacement of abdominal denticles near the ventral midline with naked cuticle.

(McGinnis et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

cnc^B.hs has embryonic head phenotype, suppressible by hth^UAS.cPa/Scer\GAL4^hs.PB

(Veraksa et al., 2000)

cnc^B.hs has mouth hook phenotype, suppressible by hth^UAS.cPa/Scer\GAL4^hs.PB

(Veraksa et al., 2000)

Enhancer of

Statement

Reference

Scer\GAL4^arm.PS/cnc^B.hs is an enhancer of mouth hook phenotype of Dfd¹⁶, Dfd^UAS.cLa

(Veraksa et al., 2000)

Scer\GAL4^arm.PS/cnc^B.hs is an enhancer of cirri phenotype of Dfd¹⁶, Dfd^UAS.cLa

(Veraksa et al., 2000)

cnc^B.hs is an enhancer of mouth hook phenotype of Dfd¹⁶, Dfd^UAS.cLa, Scer\GAL4^arm.PS

(Veraksa et al., 2000)

Other

Statement

Reference

Dfd^hs.PK, cnc^B.hs has mouth hook | ectopic phenotype

(McGinnis et al., 1998)

Dfd^hs.PK, cnc^B.hs has cirri | ectopic phenotype

(McGinnis et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

When cnc^B.hs and hth^Scer\UAS.cPa (driven by Scer\GAL4^hs.PB) are both ectopically expressed throughout the embryo, a reduction in severity of all aspects of the cnc^B.hs phenotype is observed. Although still resulting in embryonic lethality, most head skeletal elements are close to wild-type and mouth hooks are seen in all embryos. The addition of cnc^B.hs suppresses the rescuing effect seen by Dfd^Scer\UAS.cLa (when driven by Scer\GAL4^arm.PS) on Dfd¹⁶ mutants.

(Veraksa et al., 2000)

Heat shocked embryos that are heterozygous for both Dfd^hs.PK and cnc^B.hs develop ectopic cirri in only 25% of the first thoracic segments.

(McGinnis et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

cnc^B.hs

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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