FlyBase Allele Report: Dmel\chic[05205a]

General Information

Symbol

Dmel\chic^05205a

Species

D. melanogaster

Name

FlyBase ID

FBal0095483

Feature type

allele

Associated gene

Dmel\chic

Associated Insertion(s)

P{PZ}chic^05205a

Carried in Construct

Also Known As

chic⁰⁵²⁰⁵

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Wills et al., 1999)

Progenitor genotype

Associated Insertion(s)

P{PZ}chic^05205a

Cytology

Description

P{PZ} insertion 3.2kb downstream of the start of exon 1, within intron 3.

(Wills et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:5,976,783..5,979,411

(Yaniv et al., 2020, Wills et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Medioni et al., 2014)

abnormal neuroanatomy | somatic clone

(Ng and Luo, 2004)

Phenotype Manifest In

adult mushroom body | somatic clone

(Ng and Luo, 2004)

embryonic/larval neuron

(Wills et al., 1999)

filopodium (with chic²²¹)

(Gonçalves-Pimentel et al., 2011)

filopodium (with Df(2L)Gpdh1-A)

(Gonçalves-Pimentel et al., 2011)

follicle cell & actin filament | somatic clone

(Baum and Perrimon, 2001)

gamma Kenyon cell | adult stage | somatic clone

(Medioni et al., 2014)

larval intersegmental nerve

(Wills et al., 1999)

larval longitudinal connective (with chic²²¹)

(Wills et al., 1999)

motor neuron (with Df(2L)Gpdh1-A)

(Gonçalves-Pimentel et al., 2011)

Detailed Description

Statement

Reference

In contrast to wild type, single chic^05205a γ neurons (generated via MARCM) fail to properly extend their axons in the adult.

(Medioni et al., 2014)

Mean filopodium length is reduced compared to wild type in cultured primary neurons derived from chic²²¹/chic^05205a and chic^05205a/Df(2L)GpdhA embryos.

chic^05205a/Df(2L)GpdhA embryos show stalling of motor axons compared to wild type.

(Gonçalves-Pimentel et al., 2011)

Single cell γ neuron mutant clones in the mushroom body show drastic axon growth defects.

(Ng and Luo, 2004)

In mutant columnar follicle cells, levels of F-actin are markedly decreased at all cortices, although actin filaments are lost preferentially from the basal cortex.

(Baum and Perrimon, 2001)

Axon growth from homozygous dissected nerve cords cultured in vitro is reduced compared to wild-type. ISNb axons innervate proximal targets (muscles 6 and 7) and extend to contact muscle 13, but fail to reach the distal target (muscle 12) in hemizygous embryos. chic^05205a/chic²²¹ embryos show mild defects in the Fas2-positive longitudinal fascicles of the central nervous system.

(Wills et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

chic^05205a/chic²²¹ has larval longitudinal connective phenotype, enhanceable by Abl²/Abl[+]

(Wills et al., 1999)

chic^05205a/chic²²¹ has larval longitudinal connective phenotype, enhanceable by Abl²/Abl⁴

(Wills et al., 1999)

Other

Statement

Reference

chic^05205a, ena²³/ena[+] has filopodium phenotype

(Gonçalves-Pimentel et al., 2011)

capt¹⁰, chic^05205a has follicle cell | somatic clone phenotype

(Baum and Perrimon, 2001)

Abl², chic^05205a/chic²²¹ has larval longitudinal connective phenotype

(Wills et al., 1999)

Abl⁴, chic^05205a/chic²²¹ has larval longitudinal connective phenotype

(Wills et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Cultured primary neurons derived from chic^05205a/+ ena²³/+ double heterozygous embryos show a significant reduction in the number of filopodia compared to control neurons.

(Gonçalves-Pimentel et al., 2011)

Ectopic actin filaments do not form in capt¹⁰ chic^05205a double mutant follicle cell clones, and the level of F-actin in these cells appears to be similar to that in chic^05205a single mutant cells. Cells in capt¹⁰ chic^05205a double mutant follicle cell clones lose their columnar morphology and collapse, forming a thin squamous-like layer of cells. These clones can interfere with the migration of wild-type portions of the follicle cell epithelium. The double mutant clones maintain extensive contacts with adjacent wild-type cells.

(Baum and Perrimon, 2001)

The severity of the central nervous system defects of chic^05205a/chic²²¹ embryos is enhanced by Abl²/+ and further enhanced by Abl²/Abl⁴.

(Wills et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

chic⁰⁵²⁰⁵

(Yaniv et al., 2020, Medioni et al., 2014, Gonçalves-Pimentel et al., 2011, Ng and Luo, 2004)

chic^05205a

chic^P5025

(Delon and Brown, 2009)

chic^P5205

(Shields et al., 2014)

chic^l(2)5205

(Wills et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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