FlyBase Allele Report: Dmel\crb[S010409]

General Information

Symbol

Dmel\crb^S010409

Species

D. melanogaster

Name

FlyBase ID

FBal0100072

Feature type

allele

Associated gene

Dmel\crb

Associated Insertion(s)

P{lacW}crb^S010409

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Harden et al., 2002)

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

P-element activity

(Szeged Stock Center, 1998-)

Progenitor genotype

Associated Insertion(s)

P{lacW}crb^S010409

Cytology

Description

Insertion in an exon of crb, 182bp upstream of the predicted initiator methionine codon.

(Harden et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:24,295,445..24,295,445

(Harden et al., 2002, Szeged Stock Center, 1998-, Deak et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(3R)crb87-5)

(Szeged Stock Center, 1998-, Deak et al., 1997)

lethal | recessive

(Deak et al., 1997)

lethal - all die before end of pupal stage | recessive

(Szeged Stock Center, 1998-)

some die during pupal stage | recessive

(Szeged Stock Center, 1998-)

Phenotype Manifest In

amnioserosa

(Harden et al., 2002)

embryo | dorsal closure stage

(Harden et al., 2002)

embryonic hindgut

(Harden et al., 2002)

germ band

(Harden et al., 2002)

Detailed Description

Statement

Reference

crb^S010409 mutant embryos show defects in dorsal closure and germband retraction at stage 15. These mutants have an intact leading edge cytoskeleton but have a dorsal hole that is larger than wild-type embryos of similar age. The morphogenesis of crb^S010409 amnioserosa is defective and the sheet of amnioserosa cells is frequently ruptured by the hindgut. Many late crb^S010409 embyros have a persistent dorsal hole and extrusion of the gut.

(Harden et al., 2002)

Lethal phase is from the pharate adult stage onwards.

(Deak et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Rac1^V12.UAS, Scer\GAL4^hs.PB, crb^S010409 has abnormal cell shape phenotype

(Harden et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

crb^S010409 has amnioserosa phenotype, suppressible by Rac1^V12.UAS/Scer\GAL4^hs.PB

(Harden et al., 2002)

Suppressor of

Statement

Reference

crb^S010409 is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of Sac1²¹⁰⁷

(Wei et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The dorsal anterior hole in the cuticle seen in Sac1²¹⁰⁷ homozygous embryos is largely suppressed if the embryos are also heterozygous for crb^S010409 and completely suppressed if the embryos are also homozygous for crb^S010409.

(Wei et al., 2003)

When Rac1^V12.Scer\UAS is expressed by heat shock using the Scer\GAL4^hs.PB driver in a crb^S010409 homozygous mutant background, mutants no longer show contraction of the entire amnioserosa (as is seen in crb^S010409 single mutant embryos). These embryos show premature apical constriction of cells at the anterior end of the amnioserosa prior to the onset of dorsal closure in a manner similar to that seen crb^Scer\UAS.cWa overexpression driven by Scer\GAL4^332.3.

(Harden et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

crb^11A22

(Harden et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excision of the insertion can result in the reversion of the lethal phenotype.

(Harden et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

0104/09

(Deak et al., 1997)

crb^S010409

(Blankenship et al., 2007)

l(3)010409

(Harden et al., 2002)

l(3)S058104^S010409

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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