FlyBase Allele Report: Dmel\cpb[M143]

General Information

Symbol

Dmel\cpb^M143

Species

D. melanogaster

Name

FlyBase ID

FBal0103870

Feature type

allele

Associated gene

Dmel\cpb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Welte, 2000.2.23)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?148term.

(Whited et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:1,705,839..1,705,839 [+]

Nucleotide change:

G1705839A

Amino acid change:

W148term | cpb-PA; W148term | cpb-PB

Reported amino acid change:

?148term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Whited et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration

(Lucas et al., 2013)

lethal | recessive

(Welte, 2000.2.23)

Phenotype Manifest In

border follicle cell

(Lucas et al., 2013)

eye | somatic clone

(Pham et al., 2008)

eye photoreceptor cell & nucleus | somatic clone

(Whited et al., 2004)

ommatidium | somatic clone

(Pham et al., 2008)

rhabdomere | somatic clone

(Pham et al., 2008)

wing disc | somatic clone

(Fernández et al., 2011, Janody and Treisman, 2006)

Detailed Description

Statement

Reference

cpb^M143 mutants exhibit accumulate of F-actin inside border cell clusters and exhibit delayed border cell migration at stages 9 and 10. Around 10% of cpb^M143 mutant clusters disintegrate.

(Lucas et al., 2013)

Homozygous clones in the wing disc have abnormally straight boundaries and the clones are rounder than their wild-type twin spots, suggesting that the clones minimise contacts with wild-type neighbours. The mutant clone tissue is folded, but maintains the columnar shape of a polarised epithelium.

(Fernández et al., 2011)

Somatic mosaic cpb^M143 eyes exhibit a rough phenotype, with ommatidia displaying a reduced number of photoreceptor cells. In addition, many cells have widened or split rhabdomeres.

(Pham et al., 2008)

cpb^M143 mutant clones induced at the first or second larval instar could not be recovered in the wing blade primordium, although mutant clones could develop in the remainder of the wing disc.

(Janody and Treisman, 2006)

The nuclei and surrounding cytoplasm of late third instar retinal photoreceptors in cpb^M143 homozygous somatic clones often leave the retina and travel through the optic stalk into the brain.

(Whited et al., 2004)

cpb^M143 mutants survive embryogenesis.

(Grevengoed et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

cpb^M143/cpb[+] is an enhancer of abnormal cell migration phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

Other

Statement

Reference

Csk^GD9345, cpb^M143 has lethal phenotype

(Rudrapatna et al., 2014)

Abl⁴, cpb^M143 has lethal | embryonic stage phenotype

(Grevengoed et al., 2003)

Abl⁴, cpb^M143 has abnormal neuroanatomy | embryonic stage phenotype

(Grevengoed et al., 2003)

Abl⁴, cpb^M143/cpb[+] has lethal | embryonic stage phenotype

(Grevengoed et al., 2003)

Abl⁴, cpb^M143/cpb[+] has abnormal neuroanatomy | embryonic stage phenotype

(Grevengoed et al., 2003)

Abl⁴/Abl[+], cpb^M143 has abnormal neuroanatomy | embryonic stage phenotype

(Grevengoed et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

cpb^M143/cpb[+] is an enhancer of wing disc phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

Suppressor of

Statement

Reference

cpb^M143/cpb[+] is a suppressor | partially of egg | germline clone phenotype of Pkn^1T

(Ferreira et al., 2014)

cpb^M143/cpb[+] is a suppressor | partially of nurse cell | germline clone phenotype of Pkn^1T

(Ferreira et al., 2014)

cpb^M143/cpb[+] is a suppressor | partially of egg | germline clone phenotype of Pkn^2T

(Ferreira et al., 2014)

cpb^M143/cpb[+] is a suppressor | partially of nurse cell | germline clone phenotype of Pkn^2T

(Ferreira et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of cpb^M143/+ partially suppresses the shorter egg length and nurse cell F-actin accumulation seen in Pkn^1T/Pkn^1T or Pkn^2T/Pkn^2T germline clone egg chambers.

(Ferreira et al., 2014)

A cpb^M143 heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing Csk^GD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4^ptc-559.1.

(Rudrapatna et al., 2014)

Homozygous Abl⁴, heterozygous cpb^M143 double mutants lay few eggs. The crossing of cpb^M143/+ ; Abl⁴/+ mothers and fathers results in 20% of the progeny dying during embryogenesis. 15% of the progeny have minor defects in central nervous system (CNS) architecture, whereas 21% exhibit more severe CNS defects. As cpb^M143 Abl⁴ double mutants are only 6.25% of the progeny, the frequency of lethality and CNS phenotypes suggest that the mutations may dominantly enhance one another.

(Grevengoed et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

cpb^bnd3

(Delalle et al., 2005)

cpb^bnd1

(Delalle et al., 2005)

cpb^bnd2

(Delalle et al., 2005)

cpb^F44

(Whited et al., 2004)

Rescued by

cpb^M143/Df(2L)E.2 is rescued by cpb^UAS.cWa/Scer\GAL4^αTub84B.PL

(Whited et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5586

cpb^M143 cn¹ bw¹ speck¹/CyO

Kyoto

108318

cpb^M143 cn¹ b¹ speck¹/CyO

Notes on Origin

Discoverer

M. Postner

(Welte, 2000.2.23)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cpb^M143

(Ferreira et al., 2014, Rudrapatna et al., 2014, Lucas et al., 2013, Fernández et al., 2011, Sansores-Garcia et al., 2011, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Pham et al., 2008, Janody and Treisman, 2006)

cpb^M14

(Johnson et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (14)

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