FB2024_03 , released June 25, 2024
Allele: Dmel\Sharkhs.PF
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General Information
Symbol
Dmel\Sharkhs.PF
Species
D. melanogaster
Name
heat shock construct of Fernandez
FlyBase ID
FBal0104427
Feature type
allele
Associated gene
Dmel\Shark
Associated Insertion(s)
Carried in Construct
P{hs-Shark.F}
Key Links
Transgenic product class
cDNA
(Fernandez et al., 2000)
wild_type
(Fernandez et al., 2000)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Fernandez et al., 2000)
wild_type
(Fernandez et al., 2000)
Mutagen
in vitro construct
(Fernandez et al., 2000)
Progenitor genotype
Carried in construct
P{hs-Shark.F}
Cytology
Description

An Hsp70 promoter drives expression of a full length Shark cDNA which lacks 5' and 3' UTRs.

(Fernandez et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Shark
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Only 10% of embryos derived from DokPG155 germ-line clones that carry two copies of the sharkhs.PF transgene are dorsal closure defective; these embryos exhibit small anterior holes in the cuticle.

      (Biswas et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Sharkhs.PF rescues Shark1/Df(2R)Jp4

      (Fernandez et al., 2000)
      Comments

      Expression of sharkhs.PF using heat shock rescues the lethality of shark1/Df(2R)Jp4 flies.

      (Fernandez et al., 2000)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in a plasmid and transfected into S2 cells to confirm functionality of the sharkhs.PF product.

      (Fernandez et al., 2000)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Sharkhs.PF
      sharkhs.PF
      Name Synonyms
      heat shock construct of Fernandez
      (Fernandez et al., 2000)
      Secondary FlyBase IDs
        References (2)