FB2024_03 , released June 25, 2024
Allele: Dmel\bab24.UAS
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General Information
Symbol
Dmel\bab24.UAS
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Godt
FlyBase ID
FBal0120550
Feature type
allele
Associated gene
Dmel\bab2
Associated Insertion(s)
Carried in Construct
P{UAS-bab2.4}
Also Known As
UAS-bab2
Key Links
Transgenic product class
cDNA
(Couderc et al., 2002)
wild_type
(Couderc et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Couderc et al., 2002)
wild_type
(Couderc et al., 2002)
Mutagen
in vitro construct
(Couderc et al., 2002, Kopp et al., 2000)
Progenitor genotype
Carried in construct
P{UAS-bab2.4}
Cytology
Description

UASt regulatory sequences drive expression of bab2 cDNA4.

(Couderc et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
bab2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal sternite 6 & macrochaeta | male, with Scer\GAL4C-765

      (Kopp et al., 2000)

      abdominal sternite 6 & trichome | male, with Scer\GAL4C-765

      (Kopp et al., 2000)

      abdominal sternite 6 | male, with Scer\GAL4C-765

      (Kopp et al., 2000)

      abdominal sternite 7 | ectopic | male, with Scer\GAL4C-765

      (Kopp et al., 2000)

      abdominal tergite 6 & macrochaeta | male, with Scer\GAL4C-765

      (Kopp et al., 2000)

      abdominal tergite 6 & trichome | male, with Scer\GAL4C-765

      (Kopp et al., 2000)

      abdominal tergite 6 | male, with Scer\GAL4C-765

      (Kopp et al., 2000)

      abdominal tergite 7 | ectopic | male, with Scer\GAL4C-765

      (Kopp et al., 2000)

      adult abdomen & cuticle, with Scer\GAL430A

      (Bardot et al., 2002)

      adult abdomen & cuticle, with Scer\GAL4p339

      (Bardot et al., 2002)

      adult abdominal segment 4, with Scer\GAL4hs.PB

      (Couderc et al., 2002)

      adult abdominal segment 5, with Scer\GAL4hs.PB

      (Couderc et al., 2002)

      adult abdominal segment 6, with Scer\GAL4hs.PB

      (Couderc et al., 2002)

      adult cuticle & abdominal segment | male, with Scer\GAL4pnr-MD237

      (Kopp et al., 2000)

      arista, with Scer\GAL4upd1-E132

      (Bardot et al., 2002)

      basal cylinder, with Scer\GAL4upd1-E132

      (Bardot et al., 2002)

      haltere, with Scer\GAL4upd1-E132

      (Bardot et al., 2002)

      leg, with Scer\GAL4upd1-E132

      (Bardot et al., 2002)

      tarsal segment, with Scer\GAL430A

      (Bardot et al., 2002)

      tarsal segment, with Scer\GAL4p339

      (Bardot et al., 2002)

      tarsal segment | ectopic, with Scer\GAL4upd1-E132

      (Bardot et al., 2002)

      tergite, with Scer\GAL471B

      (Bardot et al., 2002)

      tergite, with Scer\GAL4bab1-Pgal4-1

      (Bardot et al., 2002)

      tergite, with Scer\GAL4hs.PB

      (Couderc et al., 2002)

      wing, with Scer\GAL430A

      (Bardot et al., 2002)

      wing, with Scer\GAL471B

      (Bardot et al., 2002)

      wing, with Scer\GAL4p339

      (Bardot et al., 2002)

      wing, with Scer\GAL4upd1-E132

      (Bardot et al., 2002)

      wing disc, with Scer\GAL4upd1-E132

      (Bardot et al., 2002)

      wing hinge, with Scer\GAL4upd1-E132

      (Bardot et al., 2002)
      Detailed Description
      Statement
      Reference

      Animals expressing bab24.Scer\UAS under the control of Scer\GAL4p339 or Scer\GAL430A have decreased pigmentation in the abdominal cuticle and the claw organs. Animals expressing bab24.Scer\UAS under the control of Scer\GAL471B or Scer\GAL4bab1-Pgal4-1 show incomplete fusion of the tergites. Animals expressing bab24.Scer\UAS under the control of Scer\GAL430A, Scer\GAL471B or Scer\GAL4p339 have wing defects. Animals expressing bab24.Scer\UAS under the control of Scer\GAL4E132 have malformed and branching legs, show a transformation of the basal cylinder and arista to tarsus and have a duplication of the haltere. The wings are shortened and broadened compared to wild type and there is an outgrowth in the hinge region of the wing. Wing discs of these animals show deformations and massive cell degeneration.

      (Bardot et al., 2002)

      Males and females expressing bab24.Scer\UAS under the control of Scer\GAL4hs.PB at 25oC lack dark pigmentation in segment A6. Little of the dark pigmentation is left in A5 and A4. Flies carrying bab24.Scer\UAS under the control of Scer\GAL4hs.PB which are raised at 18oC then shifted to 32oC during the late third larval instar/pupal stage show a "split-tergite" phenotype (as well as loss of pigmentation); the tergite primordia of all abdominal segments do not fuse (this phenotype is normally only seen in A7). Animals expressing bab24.Scer\UAS under the control of Scer\GAL4hs.PB at 25oC show reduced pigmentation in A5 and A6.

      (Couderc et al., 2002)

      Expression driven by Scer\GAL4pnr-MD237 in males causes loss of both male specific and non-sex-specific striped abdominal pigmentation. Expression driven by Scer\GAL4C-765 in males causes transformation of A6 and A7 to a more anterior identity. A7 tergite and sternites are restored. A6 sternite and tergite shape, bristles and trichomes resemble those of A5. There is no effect on the genitalia or analia of either species.

      (Kopp et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of bab24.Scer\UAS under the control of Scer\GAL4bab1-Pgal4-2 fully rescues the mutant leg phenotypes of bab2E4/Df(3L)babAr07 flies; tarsal segments 2 and 3 do not have any sex combs and tarsal segments 4 and 5 are always properly separated. bab2E4/Df(3L)babAr07 females have ovaries of reduced size which contain only a few follicles with abnormal orientation. They are subfertile and lay very few eggs. These defects are fully rescued by bab24.Scer\UAS expressed under the control of Scer\GAL4bab1-Pgal4-2. bab2E4/Df(3L)babAr07 females show increased abdominal pigmentation; segments A5 and A6 are fully pigmented. This defect is not rescued by bab24.Scer\UAS expressed under the control of Scer\GAL4bab1-Pgal4-2.

      (Bardot et al., 2002)

      The effect of Scer\GAL4pnr-MD237 in males causing loss of both male specific and non-sex-specific striped abdominal pigmentation is enhanced by abd-A,Abd-B deficiencies and partly suppressed by abd-A,Abd-B duplications. The effect of Scer\GAL4C-765 in males causing transformation of A6 and A7 to a more anterior identity is enhanced by abd-A,Abd-B deficiencies and partly suppressed by abd-A,Abd-B duplications.

      (Kopp et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      D. Godt

      (Kopp et al., 2000)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      bab-IIScer\UAS.cGa
      bab24.Scer\UAS
      bab24.UAS
      bab2Scer\UAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Godt
      (Kopp et al., 2000)
      Secondary FlyBase IDs
        References (4)