FlyBase Allele Report: Dmel\Eip93F[UAS.cLa]

General Information

Symbol

Dmel\Eip93F^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lee

FlyBase ID

FBal0123173

Feature type

allele

Associated gene

Dmel\Eip93F

Associated Insertion(s)

Carried in Construct

P{UAS-Eip93F.L}

Also Known As

UAS-E93

Key Links

Transgenic product class

start_lost

(Mou et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

start_lost

(Mou et al., 2012)

Mutagen

in vitro construct

(Lee and Baehrecke, 2001)

Progenitor genotype

Carried in construct

P{UAS-Eip93F.L}

Cytology

Description

Sequencing indicates that the initial AUG codon is mutated in this line.

(Mou et al., 2012)

UAS sequences drive expression of the Eip93F full length open reading frame.

(Lee et al., 2000)

Allele components

Component

Use(s)

Encoded product / tool

Eip93F

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo, with Scer\GAL4^h-1J3

(Lee and Baehrecke, 2001)

embryo, with Scer\GAL4^hs.PB

(Lee and Baehrecke, 2001)

wing disc, with Scer\GAL4^vg.PM

(Lee et al., 2000)

Detailed Description

Statement

Reference

When embryos are heat shocked to induce Scer\GAL4^hs.PB during embryogenesis widespread cell death occurs. When expression is driven by Scer\GAL4^h-1J3, cell death occurs in stripes in the embryos, which subsequently die at the end of embryogenesis with head defects and fusion/deletion of alternate denticle belts.

(Lee and Baehrecke, 2001)

When Eip93F^Scer\UAS.cLa is driven by Scer\GAL4^vg.PM, all animals die at the start of pupal development. Large amounts of ectopic cell death is seen in the wing and hinge regions of the wing disc. Wing disc morphogenesis is defective, such that the wing does not elongate and appears to fold back on itself. Animals die soon after head eversion during metamorphosis and exhibit defects in the presumptive adult notum and wing.

(Lee et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Eip93F^UAS.cLa, Scer\GAL4^h-1J3 has increased cell death | pair rule expression pattern phenotype, suppressible by Df(3L)H99

(Lee and Baehrecke, 2001)

NOT suppressed by

Statement

Reference

Eip93F^UAS.cLa, Scer\GAL4^h-1J3 has lethal | pair rule expression pattern phenotype, non-suppressible by Df(3L)H99

(Lee and Baehrecke, 2001)

Eip93F^UAS.cLa, Scer\GAL4^h-1J3 has increased cell death | pair rule expression pattern phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^h-1J3

(Lee and Baehrecke, 2001)

Eip93F^UAS.cLa, Scer\GAL4^h-1J3 has lethal | pair rule expression pattern phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^h-1J3

(Lee and Baehrecke, 2001)

Eip93F^UAS.cLa, Scer\GAL4^hs.PB has lethal phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hs.PB

(Lee and Baehrecke, 2001)

Eip93F^UAS.cLa, Scer\GAL4^hs.PB has increased cell death phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hs.PB

(Lee and Baehrecke, 2001)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Eip93F^UAS.cLa, Scer\GAL4^h-1J3 has embryo phenotype, non-suppressible by Df(3L)H99

(Lee and Baehrecke, 2001)

Eip93F^UAS.cLa, Scer\GAL4^h-1J3 has embryo phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^h-1J3

(Lee and Baehrecke, 2001)

Eip93F^UAS.cLa, Scer\GAL4^hs.PB has embryo phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hs.PB

(Lee and Baehrecke, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Cell death phenotype is not suppressed by removal of rpr, W and grim (i.e. Df(3L)H99), though the remaining cell death does not show the nuclear hallmarks or programmed cell death.

(Lee and Baehrecke, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^fkh.PH/Eip93F^UAS.cLa rescues Df(3R)93F^x2

(Lee et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Eip93F^Scer\UAS.cLa

Eip93F^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Lee

(Lee and Baehrecke, 2001)

Secondary FlyBase IDs

References (3)

Report Sections

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