7kb SalI-HindIII genomic fragment containing ovo in which ovo nucleotides 3666-5338 have been replaced with the ovo cDNA sequences. The resulting construct lacks ovo intron IIb sequences.
wild-type
ovo+2b rescues ovoD1rv23
ovo+2b rescues ovoD1rv37
A single copy of ovo+2b fully restores the fertility of ovoD1rv23 and ovoD1rv37 females.