FlyBase Allele Report: Dmel\zpg[z-2533]

General Information

Symbol

Dmel\zpg^z-2533

Species

D. melanogaster

Name

FlyBase ID

FBal0138099

Feature type

allele

Associated gene

Dmel\zpg

Associated Insertion(s)

Carried in Construct

Also Known As

zpg^z2533

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tazuke et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: K239term.

(Tazuke et al., 2002)

Nucleotide substitution: A931T.

(Tazuke et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:6,605,067..6,605,067 [-]

Nucleotide change:

A6605067T

Reported nucleotide change:

A931T

Amino acid change:

K239term | zpg-PA; K239term | zpg-PB

Reported amino acid change:

K239term

(Tazuke et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage (with zpg^z-5352)

(Smendziuk et al., 2015)

decreased occurrence of cell division | adult stage (with zpg^z-5352)

(Smendziuk et al., 2015)

increased cell number | adult stage (with zpg^z-5352)

(Smendziuk et al., 2015)

increased occurrence of cell division | adult stage (with zpg^z-5352)

(Smendziuk et al., 2015)

male sterile

(Tazuke et al., 2002)

sterile (with zpg^z-5352)

(Smendziuk et al., 2015)

viable

(Tazuke et al., 2002)

Phenotype Manifest In

cyst progenitor cell (with zpg^z-5352)

(Smendziuk et al., 2015)

female germline cell

(Chau et al., 2009)

male germline stem cell (with zpg^z-5352)

(Smendziuk et al., 2015)

secondary spermatogonium (with zpg^z-5352)

(Smendziuk et al., 2015)

somatic cell of testis (with zpg^z-5352)

(Smendziuk et al., 2015)

spectrosome | ectopic (with zpg^z-5352)

(Smendziuk et al., 2015)

testis (with zpg^z-5352)

(Smendziuk et al., 2015)

Detailed Description

Statement

Reference

zpg^z-5352/zpg^z-2533 testes are rudimentary and have a significant reduction in male germline stem cell number compared with controls. Many zpg^z-5352/zpg^z-2533 mutant testes do not contain GSCs at 1 day post eclosion but most contain germ cells. zpg^z-5352/zpg^z-2533 mutant testes display a large increase in somatic cyst stem cells and early-stage somatic cells, and a significant decrease in late-stage somatic cells compared with controls. There is a significant increase in the number of proliferating somatic cells, but the number of proliferating somatic cyst stem cells is decreased. Cadherin-mediated cell-cell adhesion is unaffected in zpg null testes.

In zpg^z-5352/zpg^z-2533 testes, 2-cell stage spermatogonia display ectopic spectrosomes alongside early fusomes, indicating defective germline differentiation, and germline cyst encapsulation was defective.

(Smendziuk et al., 2015)

Mutant ovaries contain an average of only 6 +/- 0.3 germline cells per germarium.

(Chau et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

snf¹⁴⁸ ; zpg^z-2533 double mutant ovaries are not tumourous and an average of only 11 +/- 0.4 germline cells per germarium, similar to the zpg^z-2533 single mutant phenotype.

(Chau et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

zpg¹

(Tazuke et al., 2002)

zpg²

(Tazuke et al., 2002)

Rescued by

zpg^z-5352/zpg^z-2533 is rescued by zpg^GFP

(Smendziuk et al., 2015)

Comments

The expression of zpg^T:Avic\GFP is able to fully rescue the spermatogenesis phenotype of zpg^z-5352/zpg^z-2533 and restore fertility to wild-type levels.

(Smendziuk et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9918

zpg^z-2533 st¹/TM3, Sb¹

Notes on Origin

Discoverer

B. Wakimoto D. Lindsley C. Zuker

(Tazuke et al., 2002)

Comments

Strong allele. zpg^z-2533 and zpg^z-2552 have identical changes in the zpg coding region but zpg^z-2552 has slightly more germ cells in the gonad.

(Tazuke et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

zpg^z-2533

(Hudry et al., 2019, Smendziuk et al., 2015, Mukai et al., 2011)

zpg^z2533

(Chau et al., 2009, Gilboa et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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