FlyBase Allele Report: Dmel\wgn[RNAi.UAS]

General Information

Symbol

Dmel\wgn^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0138107

Feature type

allele

Associated gene

Dmel\wgn

Associated Insertion(s)

Carried in Construct

P{UAS-wgn.RNAi}

Also Known As

UAS-Wengen-IR, UAS-Wgn-IR

Key Links

Transgenic product class

RNAi_reagent

(Kanda et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kanda et al., 2002)

Mutagen

in vitro construct

(Kanda et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-wgn.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of two copies of wgn cDNA sequences in a head-to-head inverted repeat orientation.

(Kanda et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

wgn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

No evidence of NMJ degeneration is seen when wgn^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155 and the number of boutons is comparable to wild type.

(Keller et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/wgn^RNAi.UAS is a suppressor | partially of abnormal neuroanatomy phenotype of Ank2^f02001

(Keller et al., 2011)

Scer\GAL4^elav-C155/wgn^RNAi.UAS is a suppressor | partially of abnormal neurophysiology phenotype of Ank2^f02001

(Keller et al., 2011)

wgn^RNAi.UAS/Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Ras85D^KP

(Wu et al., 2009)

wgn^RNAi.UAS, Scer\GAL4^Act5C.PI is a suppressor | partially of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, l(2)gl⁴

(Igaki et al., 2006)

wgn^RNAi.UAS/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Kanda et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^hh-Gal4/wgn^RNAi.UAS is a non-suppressor of neoplasia | larval stage phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

wgn^RNAi.UAS, Scer\GAL4^Act5C.PP is a non-suppressor of neoplasia phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^GD4689

(Andersen et al., 2015)

wgn^RNAi.UAS/Scer\GAL4^ey.PU is a non-suppressor of visible phenotype of Scer\GAL4^pnr.PU, ben^EP1651

(Ma et al., 2014)

wgn^RNAi.UAS/Scer\GAL4^ey.PU is a non-suppressor of visible phenotype of Scer\GAL4^ey.PU, ben^d09936

(Ma et al., 2014)

wgn^RNAi.UAS, Scer\GAL4^dpp.blk1 is a non-suppressor of visible phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

wgn^RNAi.UAS, Scer\GAL4^pnr-MD237 is a non-suppressor of visible phenotype of CYLD^UAS.cXa, Scer\GAL4^pnr-MD237

(Xue et al., 2007)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/wgn^RNAi.UAS is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Ank2^f02001

(Keller et al., 2011)

wgn^RNAi.UAS/Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Ras85D^KP

(Wu et al., 2009)

wgn^RNAi.UAS/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Kanda et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^hh-Gal4/wgn^RNAi.UAS is a non-suppressor of wing disc phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

wgn^RNAi.UAS/Scer\GAL4^pnr.PU is a non-suppressor of scutellum phenotype of Scer\GAL4^ey.PU, ben^EP1651

(Ma et al., 2014)

wgn^RNAi.UAS/Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of Scer\GAL4^ey.PU, ben^d09936

(Ma et al., 2014)

wgn^RNAi.UAS, Scer\GAL4^dpp.blk1 is a non-suppressor of wing phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

Scer\GAL4^elav-C155/wgn^RNAi.UAS is a non-suppressor of microtubule | third instar larval stage phenotype of Ank2^f02001

(Keller et al., 2011)

Scer\GAL4^elav-C155/wgn^RNAi.UAS is a non-suppressor of axon | third instar larval stage phenotype of Ank2^f02001

(Keller et al., 2011)

wgn^RNAi.UAS, Scer\GAL4^pnr-MD237 is a non-suppressor of scutellum phenotype of CYLD^UAS.cXa, Scer\GAL4^pnr-MD237

(Xue et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wgn^{dsRNA.Scer\UAS} does not suppress the rough eye phenotype seen when ben^d09936 is expressed under the control of Scer\GAL4^ey.PU.

Expression of wgn^{dsRNA.Scer\UAS} does not suppress the small scutellum phenotype seen when ben^EP1651 is expressed under the control of Scer\GAL4^pnr.PU.

(Ma et al., 2014)

Expression of wgn^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 partially suppresses the NMJ degeneration seen in Ank2^f02001 mutant third instar larvae. The axonal blockages and microtubule disruption are not suppressed.

Expression of wgn^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 partially suppresses the synaptic transmission defects seen in Ank2^f02001 mutants. A near complete rescue in the number of small EPSPs is seen, however average EPSP amplitude is not completely restored to wild type levels.

(Keller et al., 2011)

The eye phenotype in Ras85D^KP mutant flies is partially suppressed by ectopic expression of wgn^{dsRNA.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF.

(Wu et al., 2009)

Co-expression of wgn^{dsRNA.Scer\UAS} does not suppress the reduced scutellum phenotype caused by expression of CYLD^Scer\UAS.cXa under the control of Scer\GAL4^pnr-MD237.

(Xue et al., 2007)

Expression of wgn^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^Act5C.PI) using the FLP/FRT system partially suppresses metastatic behaviour in l(2)gl⁴ mutants expressing Ras85D^V12.Scer\UAS.

(Igaki et al., 2006)

Co-expression of wgn^{dsRNA.Scer\UAS} suppresses the small eye phenotype caused by expression of egr^GS9830 under the control of Scer\GAL4^GMR.PF.

(Kanda et al., 2002)

Xenogenetic Interactions

Statement

Reference

Co-expression of wgn^{dsRNA.Scer\UAS} does not suppress the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

(Marchal et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid P{UAS-wengen-IR} and transfected into S2 cells.

(Kanda et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

wgn^RNAi.UAS

wgn^{dsRNA.Scer\UAS}

wgn^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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