FlyBase Allele Report: Dmel\Atpα[D369N.UAS]

General Information

Symbol

Dmel\Atpα^D369N.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0138588

Feature type

allele

Associated gene

Dmel\Atpα

Associated Insertion(s)

Carried in Construct

P{UAS-Atpα.D369N}

Also Known As

UAS-dnATPase

Key Links

Transgenic product class

missense_variant

(Sun et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Sun et al., 2001)

Mutagen

in vitro construct

(Sun et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Atpα.D369N}

Cytology

Description

UASt regulatory sequences drive expression of Atpα with the amino acid replacement D369N. The nucleotide substitution that generates the amino acid change also results in the formation of a KpnI site.

(Sun et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atpα

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with Scer\GAL4^nrv2.PS

(Sun et al., 2001)

abnormal circadian behavior | adult stage, with Scer\GAL4^P2.4.Pdf

(Parisky et al., 2008)

abnormal neurophysiology | larval stage, with Scer\GAL4^futsch-C380

(Parisky et al., 2008)

chemical sensitive, with Scer\GAL4^nrv2.PS

(Sun et al., 2001)

lethal, with Scer\GAL4^nrv1.PS

(Sun et al., 2001)

partially lethal, with Scer\GAL4^nrv2.PS

(Sun et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of Atpα^{D369N.Scer\UAS} with Scer\GAL4^C380 results in an increase in the resting membrane potential and the fire-rate response of larval motorneurons. Expression of Atpα^{D369N.Scer\UAS} with Scer\GAL4^P2.4.Pdf results in a reduction in total sleep and an increase in sleep latency during the day and night, compared to controls.

(Parisky et al., 2008)

Animals expressing Atpα^{D369N.Scer\UAS} under the control of Scer\GAL4^nrv1.PS die by the late first larval instar. Animals expressing Atpα^{D369N.Scer\UAS} under the control of Scer\GAL4^nrv2.PS are more sensitive to ouabain than wild-type animals at all concentrations of ouabain tested. Adults expressing Atpα^{D369N.Scer\UAS} under the control of Scer\GAL4^nrv2.PS show temporary paralysis or uncontrolled wing beating after brief mechanical stimulation.

(Sun et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ATPα^DN

(Wong et al., 2021)

Atpα^{D369N.Scer\UAS}

Atpα^D369N.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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