FlyBase Allele Report: Dmel\Diap1[22-8S]

General Information

Symbol

Dmel\Diap1^22-8S

Species

D. melanogaster

Name

FlyBase ID

FBal0146759

Feature type

allele

Associated gene

Dmel\Diap1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: C415Y.

(Ryoo et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:16,038,745..16,038,745 [-]

Nucleotide change:

G16038745A

Amino acid change:

Reported amino acid change:

C415Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ryoo et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | larval stage | somatic clone

(Ryoo et al., 2004)

increased cell death | somatic clone

(Ryoo et al., 2004)

Phenotype Manifest In

female germline stem cell | germline clone

(Xing et al., 2015)

wing disc posterior compartment | somatic clone

(Ryoo et al., 2004)

Detailed Description

Statement

Reference

As early as 3 days after clone induction, no Diap1^22-8S mutant clones are observed in the female germline stem cell niche, while wild-type control clones are recovered in 15% of the germaria examined.

(Xing et al., 2015)

Somatic clones of th^22-8S homozygous cells in the wing disc express a cell death marker 18 hours after induction and are lost from the disc by 72 hours after induction. Despite this increased cell death, the resulting adults have normal wings.

(Ryoo et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

BacA\p35^UAS.cHa, Diap1^22-8S, Scer\GAL4^ey.PH has hyperplasia | somatic clone phenotype

(Ryoo et al., 2004)

BacA\p35^UAS.cHa, Diap1^22-8S, Scer\GAL4^en-e16E has hyperplasia | somatic clone | cell non-autonomous phenotype

(Ryoo et al., 2004)

BacA\p35^UAS.cHa, Diap1^22-8S, Scer\GAL4^en-e16E has hyperplasia | somatic clone | larval stage phenotype

(Ryoo et al., 2004)

BacA\p35^UAS.cHa, Diap1^22-8S, Scer\GAL4^en-e16E has abnormal size | somatic clone | larval stage phenotype

(Ryoo et al., 2004)

BacA\p35^UAS.cHa, Diap1^22-8S, Scer\GAL4^en-e16E has increased occurrence of cell division | somatic clone | larval stage phenotype

(Ryoo et al., 2004)

BacA\p35^UAS.cHa, Diap1^22-8S, Scer\GAL4^en-e16E has increased occurrence of cell division | somatic clone | cell non-autonomous | larval stage phenotype

(Ryoo et al., 2004)

Phenotype Manifest In

Other

Statement

Reference

BacA\p35^UAS.cHa, Diap1^22-8S, Scer\GAL4^ey.PH has eye | somatic clone phenotype

(Ryoo et al., 2004)

BacA\p35^UAS.cHa, Diap1^22-8S, Scer\GAL4^en-e16E has wing disc posterior compartment | somatic clone phenotype

(Ryoo et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Survival of th^22-8S homozygous somatic clones induced in the posterior compartment of the wing disc is rescued by BacA\p35^Scer\UAS.cHa; Scer\GAL4^en-e16E. This is accompanied by an increase in proliferation of cells of the posterior compartment (as measure by BrdU incorporation), an effect seen most strongly in cells surrounding the clones. The resulting wing discs have a posterior compartment significantly larger than wild-type at late third instar.

th^22-8S homozygous somatic clones induced in the eye disc in the presence of BacA\p35^Scer\UAS.cHa; Scer\GAL4^ey.PH cause outgrowths in the adult eye.

(Ryoo et al., 2004)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Diap1^22-8S

diap1^22-8

(Ryoo et al., 2004)

diap1^22-8S

(Xing et al., 2015, Ryoo et al., 2002)

diap1^22-8s

(Ryoo et al., 2004)

th^22-8S

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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