FlyBase Allele Report: Dmel\MTF-1[140-1R]

General Information

Symbol

Dmel\MTF-1^140-1R

Species

D. melanogaster

Name

FlyBase ID

FBal0147797

Feature type

allele

Associated gene

Dmel\MTF-1

Associated Insertion(s)

Carried in Construct

Also Known As

dMTF-1^140-1R

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

FLPase

(Egli et al., 2003)

I-SceI

(Egli et al., 2003)

I-CreI

(Egli et al., 2003)

gene targeting by homologous recombination

(Egli et al., 2003)

Progenitor genotype

Cytology

Description

4.1 kb deletion that removes part of exon 1, the whole of exon 2 and the intervening intron of the MTF-1 gene.

(Egli et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Friedreich ataxia 1

modeled by fh^RNAi.UAS.cAa

(Soriano et al., 2016)

exacerbates Parkinson's disease

modeled by park²⁵

(Saini et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Selvaraj et al., 2005)

chemical resistant, with MTF-1^UAS.cEa, Scer\GAL4^NP2020

(Bahadorani et al., 2010)

chemical sensitive

(Bahadorani et al., 2010, Yepiskoposyan et al., 2006, Egli et al., 2003)

chemical sensitive, with MTF-1^UAS.cEa, Scer\GAL4^NP2020

(Bahadorani et al., 2010)

chemical sensitive, with MTF-1^UAS.cEa, Scer\GAL4^NP6219

(Bahadorani et al., 2010)

chemical sensitive | recessive

(Balamurugan et al., 2004)

fertile

(Egli et al., 2003)

fertile (with Df(3L)AC1)

(Egli et al., 2003)

lethal - all die before end of larval stage

(Selvaraj et al., 2005)

short lived | chemical conditional

(Bahadorani et al., 2010)

short lived | chemical conditional, with MTF-1^UAS.cEa, Scer\GAL4^NP2020

(Bahadorani et al., 2010)

short lived | chemical conditional, with MTF-1^UAS.cEa, Scer\GAL4^NP6219

(Bahadorani et al., 2010)

some die during third instar larval stage

(Selvaraj et al., 2005)

viable

(Egli et al., 2003)

viable (with Df(3L)AC1)

(Egli et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

The life-span of MTF-1^140-1R mutant flies is not affected on zinc-supplemented medium, but significantly reduced on normal food or cadmium-supplemented medium compared with wild-type flies.

Overexpression of MTF-1^Scer\UAS.cEa in the gut under the control of Scer\GAL4^NP2020 moderately increases resistance to iron and cadmium toxicity in an MTF-1^140-1R mutant background but sensitises flies to zinc supplementation.

Overexpression of MTF-1^Scer\UAS.cEa in hemocytes under the control of Scer\GAL4^clu-NP6219 fully rescues the cadmium-sensitive phenotype of MTF-1^140-1R mutant flies but increases sensitivity to zinc toxicity.

(Bahadorani et al., 2010)

Iron treatment results in substantial ferritin expression in the anterior midgut of MTF-1^140-1R knockdown mutant larvae.

(Gutiérrez et al., 2010)

Homozygous flies show a metal sensitive phenotype (they are sensitive to zinc, cadmium and copper).

(Yepiskoposyan et al., 2006)

MTF-1^140-1R mutants are developmentally arrested and die at second or third instar larval stages when the concentration of copper chelator bathocuproinedisulfonate reaches 50υM in the food.

(Selvaraj et al., 2005)

MTF-1^140-1R homozygotes raised on food containing a copper chelator (50μM bathocuproine disulfonate) do not survive to eclosion.

MTF-1^140-1R homozygotes raised on food containing Cu²⁺ (1mM or 2mM ), Cd²⁺ (100μM or 200μM) or Zn²⁺ (2mM, 4mM or 6mM) show greatly reduced survival or no survival to eclosion compared to heterozygous controls.

(Balamurugan et al., 2004)

Mutants are more sensitive to elevated concentrations of cadmium, copper or zinc than wild-type flies. Whereas 30-4-% of embryos laid by wild-type flies exposed to either 100uM of CdCl₂, 1mM of CuSO₄, or 6mM ZnCl2 survive to adulthood, no eggs laid by mutant animals survive in these conditions. Mutant animals are also more sensitive to Copper depletion. When mutant animals are exposed to Copper chelators BCS (100uM) and TTM (5uM) no embryos laid by these animals survive (compared to 40-50% for eggs laid by wild-type animals). Mutant larvae die at first and second instar larval stages. Mutant larvae also exhibit a dramatically extended larval period on copper depletion, some mutant larvae still remaining after 32 days. These larvae are the size of third instar larvae, but still exhibit features (e.g. the tracheal system and mouth) of second instar larvae.

(Egli et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

MTF-1^140-1R, park²⁵ has lethal | pupal stage phenotype, suppressible by MTF-1^αTub84B.PS

(Saini et al., 2011)

MTF-1^140-1R has chemical sensitive phenotype, suppressible by Scer\GAL4^Act.PU/CG10505^UAS.cYa

(Yepiskoposyan et al., 2006)

MTF-1^140-1R has chemical sensitive phenotype, suppressible by Scer\GAL4^Act.PU/ZnT35C^a.UAS

(Yepiskoposyan et al., 2006)

MTF-1^140-1R has lethal | third instar larval stage phenotype, suppressible by Scer\GAL4^Act5C.PU/Ctr1B^UAS.cSa

(Selvaraj et al., 2005)

MTF-1^140-1R has abnormal developmental rate phenotype, suppressible by Scer\GAL4^Act5C.PU/Ctr1B^UAS.cSa

(Selvaraj et al., 2005)

MTF-1^140-1R has chemical sensitive | recessive phenotype, suppressible by Scer\GAL4^Act.PU/Hsap\MTF1^UASp.cBa

(Balamurugan et al., 2004)

NOT suppressed by

Statement

Reference

MTF-1^140-1R has chemical sensitive phenotype, non-suppressible by ZnT35C^b.UAS/Scer\GAL4^Act.PU

(Yepiskoposyan et al., 2006)

Other

Statement

Reference

MTF-1^140-1R, park²⁵ has lethal | pupal stage phenotype

(Saini et al., 2011)

MTF-1^140-1R, fbl¹ has abnormal locomotor behavior phenotype

(Gutiérrez et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

park²⁵/MTF-1^140-1R double mutants are synthetic lethal at the pupal stage. Raising these flies on 15Mm N-acetylcysteine, a precursor to glutathione overcomes the synthetic lethality seen in these double mutants. the presence of ascorbate, zinc, or metal chelators of copper and iron does not rescue synthetic lethality.

The presence of MTF-1^αTub84B.PS rescues the synthetic lethality found in park²⁵/MTF-1^140-1R double mutants.

(Saini et al., 2011)

fbl¹, MTF-1^140-1R homozygotes are viable but are sensitive to excess metals and show a behavioral walking phenotype.

(Gutiérrez et al., 2010)

Expression of CG10505^Scer\UAS.cYa under the control of Scer\GAL4^Act in a MTF-1^140-1R mutant background partially rescues the zinc and copper sensitivity phenotype of MTF-1^140-1R animals, but has no effect on the sensitivity of these animals to cadmium.

Expression of ZnT35C^b.Scer\UAS under the control of Scer\GAL4^Act has no effect on the metal sensitivity of MTF-1^140-1R flies.

Expression of ZnT35C^a.Scer\UAS under the control of Scer\GAL4^Act partially rescues the zinc sensitivity phenotype of MTF-1^140-1R flies, but has no effect on the sensitivity of these animals to cadmium.

(Yepiskoposyan et al., 2006)

Constitutive expression of Ctr1B^Scer\UAS.cSa, under the regulation of Scer\GAL4^Act5C rescues the developmental arrest and larval lethality of MTF-1^140-1R mutants under copper depletion, with several viable MTF-1^140-1R Ctr1B^Scer\UAS.cSa (Scer\GAL4^Act5C) flies surviving on food containing 50 or even 100υM bathocuproinedisulfonate (a copper chelator). The rescued flies are normal and fertile.

(Selvaraj et al., 2005)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\MTF1^{Scer\UAS.P\T.cBa} under the control of Scer\GAL4^Act in MTF-1^140-1R homozygotes partially rescues the sensitivity of MTF-1^140-1R to copper depletion (caused by the copper chelator bathocuproine disulfonate), as increase survival to eclosion is seen.

The sensitivity of MTF-1^140-1R homozygotes to heavy metal ions is partially rescued by expression of Hsap\MTF1^{Scer\UAS.P\T.cBa} under the control of Scer\GAL4^Act; increased survival is seen on exposure to 1mM or 2mM Cu²⁺ or on exposure to 100μM or 200μM Cd²⁺ compared to MTF-1^140-1R homozygotes.

(Balamurugan et al., 2004)

Complementation and Rescue Data

Partially rescued by

MTF-1^140-1R is partially rescued by MTF-1^UAS.cEa/Scer\GAL4^NP2020

(Bahadorani et al., 2010)

MTF-1^140-1R is partially rescued by Scer\GAL4^NP6219/MTF-1^UAS.cEa

(Bahadorani et al., 2010)

Comments

(Bahadorani et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9241

MTF-1^140-1R

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

MTF-1 KO

(Yepiskoposyan et al., 2006)

MTF-1^140-1R

(Soriano et al., 2016, Saini et al., 2011, Bahadorani et al., 2010, Gutiérrez et al., 2010, Yepiskoposyan et al., 2006, Balamurugan et al., 2004)

dMTF-1^140-1R

(Balamurugan et al., 2009, Selvaraj et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (9)

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