FlyBase Allele Report: Dmel\mir-14[Δ1]

General Information

Symbol

Dmel\mir-14^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0150343

Feature type

allele

Associated gene

Dmel\mir-14

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)

Mutagen

Progenitor genotype

mir-14^k10213

(Xu et al., 2003)

Cytology

Description

Imprecise excision of the mir-14^k10213 insertion produces a 533bp deletion of the entire mir-14 gene.

(Xu et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult adipose system

(Xu et al., 2003)

anterior pupal spiracle

(Varghese and Cohen, 2007)

embryonic/larval dorsal tracheal branch | third instar larval stage

(Kim et al., 2014)

salivary gland | pupal stage

(Nelson et al., 2014)

terminal tracheal cell | third instar larval stage

(Kim et al., 2014)

wing blade

(Kim et al., 2014)

wing blade posterior compartment

(Kim et al., 2014)

Detailed Description

Statement

Reference

mir-14^Δ1/mir-14^Δ1 flies show significant increases in posterior (P) wing compartment size and posterior / anterior (A) wing size ratio compared to controls. mir-14^Δ1/mir-14^Δ1 mutant wings are significantly larger than wild type wings.

In wild type third instar larvae, two dorsal tracheal branches fuse to give rise to two terminal cells with multiple branches; a significantly increased percentage of mir-14^Δ1/mir-14^Δ1 mutant terminal branches have excess (>2) terminal cells.

(Kim et al., 2014)

Salivary gland cell size is normal in homozygous wandering third instar larvae.

Salivary gland fragments persist 24 hours after puparium in homozygous animals (no residual salivary gland material is present in wild-type animals at this stage).

Homozygotes show a reduction in autophagy in the salivary glands, but not in the fat body, 14 hours after pupariation.

(Nelson et al., 2014)

Mutant adults have an elevated total body fat to total body protein ratio compared to controls.

Mutant adults are more sensitive to starvation compared to controls.

(Varghese et al., 2010)

Mutants show no defects in targeting of the DL1 glomerulus in the antennal lobe.

(Berdnik et al., 2008)

Mutants show significantly reduced survival to adulthood and also have a reduced adult life span compared to wild-type flies.

Mutant animals show defects in anterior spiracle eversion at the larval-pupal transition; 52% of mutants show a failure to evert one or both spiracles.

(Varghese and Cohen, 2007)

Homozygotes hatch at normal frequency, larvae survive to pupal stages at a rate similar to that of wild-type. Most mutant animals die during pupal development. Those that survive exhibit delayed eclosion and have a decreased mean and maximal lifespan. This decrease is particularly marked in females. Homozygotes are also much more susceptible than wild-type to being killed by salt stress. Homozygous adults have enlarged adipocytes. Animals have increased levels of Triacylglycerol and Diacylglycerol.

(Xu et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-14^Δ1 has visible phenotype, suppressible | partially by hh^HMS00492/Scer\GAL4^hh-Gal4

(Kim et al., 2014)

mir-14^Δ1 has abnormal size | adult stage phenotype, suppressible | partially by hh^HMS00492/Scer\GAL4^hh-Gal4

(Kim et al., 2014)

mir-14^Δ1 has increased cell number | third instar larval stage phenotype, suppressible by hh[+]/hh^AC

(Kim et al., 2014)

mir-14^Δ1 has increased cell number | third instar larval stage phenotype, suppressible by hh^HMS00492/Scer\GAL4^hh-Gal4

(Kim et al., 2014)

mir-14^Δ1 has abnormal starvation stress response | adult stage phenotype, suppressible | partially by sug^GD11090/Scer\GAL4^Ilp2.215-3

(Varghese et al., 2010)

mir-14^Δ1 has partially lethal phenotype, suppressible by EcR^V559fs/EcR[+]

(Varghese and Cohen, 2007)

mir-14^Δ1 has short lived phenotype, suppressible by EcR^V559fs/EcR[+]

(Varghese and Cohen, 2007)

Enhancer of

Statement

Reference

mir-14^Δ1/mir-14^Δ1 is an enhancer of visible | dominant phenotype of hh^Mrt

(Kim et al., 2014)

NOT Enhancer of

Statement

Reference

mir-14[+]/mir-14^Δ1 is a non-enhancer of visible | dominant phenotype of Bx¹

(Bejarano et al., 2008)

Suppressor of

Statement

Reference

mir-14^Δ1/mir-14^Δ1 is a suppressor of visible phenotype of Scer\GAL4^hh-Gal4, hh^HMS00492

(Kim et al., 2014)

mir-14^Δ1/mir-14^Δ1 is a suppressor of abnormal size | adult stage phenotype of hh^HMS00492

(Kim et al., 2014)

NOT Suppressor of

Statement

Reference

mir-14[+]/mir-14^Δ1 is a non-suppressor of visible | dominant phenotype of Bx¹

(Bejarano et al., 2008)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

mir-14^Δ1 has salivary gland | pupal stage phenotype, non-enhanceable by Atg6^GD11647/Scer\GAL4^fkh.PU

(Nelson et al., 2014)

Suppressed by

Statement

Reference

mir-14^Δ1 has wing blade phenotype, suppressible | partially by hh^HMS00492/Scer\GAL4^hh-Gal4

(Kim et al., 2014)

mir-14^Δ1 has embryonic/larval dorsal tracheal branch | third instar larval stage phenotype, suppressible by hh[+]/hh^AC

(Kim et al., 2014)

mir-14^Δ1 has terminal tracheal cell | third instar larval stage phenotype, suppressible by hh[+]/hh^AC

(Kim et al., 2014)

mir-14^Δ1 has embryonic/larval dorsal tracheal branch | third instar larval stage phenotype, suppressible by hh^HMS00492/Scer\GAL4^hh-Gal4

(Kim et al., 2014)

mir-14^Δ1 has terminal tracheal cell | third instar larval stage phenotype, suppressible by hh^HMS00492/Scer\GAL4^hh-Gal4

(Kim et al., 2014)

mir-14^Δ1 has salivary gland | pupal stage phenotype, suppressible by IP3K2^Δ1/IP3K2^Δ1

(Nelson et al., 2014)

mir-14^Δ1 has anterior pupal spiracle phenotype, suppressible by EcR^V559fs/EcR[+]

(Varghese and Cohen, 2007)

NOT suppressed by

Statement

Reference

mir-14^Δ1 has salivary gland | pupal stage phenotype, non-suppressible by Atg6^GD11647/Scer\GAL4^fkh.PU

(Nelson et al., 2014)

Enhancer of

Statement

Reference

mir-14^Δ1/mir-14^Δ1 is an enhancer of wing blade anterior compartment phenotype of hh^Mrt

(Kim et al., 2014)

mir-14^Δ1/mir-14^Δ1 is an enhancer of wing blade phenotype of hh^Mrt

(Kim et al., 2014)

mir-14^Δ1/mir-14^Δ1 is an enhancer of wing vein L2 phenotype of hh^Mrt

(Kim et al., 2014)

mir-14^Δ1/mir-14^Δ1 is an enhancer of wing vein L3 phenotype of hh^Mrt

(Kim et al., 2014)

mir-14^Δ1/mir-14^Δ1 is an enhancer of wing margin bristle phenotype of hh^Mrt

(Kim et al., 2014)

mir-14^Δ1 is an enhancer of eye phenotype of rpr^GMR.PH

(Xu et al., 2003)

NOT Enhancer of

Statement

Reference

mir-14[+]/mir-14^Δ1 is a non-enhancer of wing margin phenotype of Bx¹

(Bejarano et al., 2008)

Suppressor of

Statement

Reference

mir-14^Δ1/mir-14^Δ1 is a suppressor of wing blade posterior compartment phenotype of Scer\GAL4^hh-Gal4, hh^HMS00492

(Kim et al., 2014)

mir-14^Δ1/mir-14^Δ1 is a suppressor of wing blade phenotype of Scer\GAL4^hh-Gal4, hh^HMS00492

(Kim et al., 2014)

NOT Suppressor of

Statement

Reference

mir-14[+]/mir-14^Δ1 is a non-suppressor of wing margin phenotype of Bx¹

(Bejarano et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

mir-14^Δ1/mir-14^Δ1 significantly suppresses the wing curvature and decreases in posterior wing compartment size seen in Scer\GAL4^hh-Gal4>hh^HMS00492 flies; these flies actually show a significant increase in posterior wing compartment size (and P/A wing size ratio) compared to controls. Expression of hh^HMS00492 driven by Scer\GAL4^hh-Gal4 significantly (partially) suppresses the increases in wing size seen in mir-14^Δ1/mir-14^Δ1 mutants.

mir-14^Δ1/mir-14^Δ1 significantly enhances wing phenotypes in hh^Mrt/+ flies, with 55% showing class I phenotypes, 31% showing class II phenotypes and 14% showing class III phenotypes.

hh^AC/+ or expression of hh^HMS00492 driven by Scer\GAL4^hh-Gal4 significantly suppresses the increased percentage of mir-14^Δ1/mir-14^Δ1 mutant dorsal tracheal branches that have excess (>2) terminal cells.

(Kim et al., 2014)

Expression of Atg6^GD11647 under the control of Scer\GAL4^fkh.PU does not significantly alter the persistent salivary gland phenotype seen in mir-14^Δ1 homozygotes 24 hours after pupariation.

The reduction in salivary gland autophagy and persistent salivary gland phenotype which is seen in mir-14^Δ1 homozygous pupae is suppressed in IP3K2^Δ1/Y ; mir-14^Δ1/mir-14^Δ1 double mutant pupae.

(Nelson et al., 2014)

Expression of Ilp3^Scer\UAS.cIa under the control of Scer\GAL4^Ilp2.215-3 rescues the elevated total body fat to total body protein ratio seen in mir-14^Δ1 adults.

Expression of sug^GD11090 under the control of Scer\GAL4^Ilp2.215-3 partially rescues the elevated total body fat to total body protein ratio seen in mir-14^Δ1 adults.

The increased sensitivity of mir-14^Δ1 adults to starvation can be partially suppressed by expression of sug^GD11090 under the control of Scer\GAL4^Ilp2.215-3.

(Varghese et al., 2010)

The reduced survival to adulthood and shorter than normal life span of mir-14^Δ1 flies is suppressed by one copy of EcR^V559fs. The defects in anterior spiracle eversion seen in mir-14^Δ1 pupae are almost completely suppressed by one copy of EcR^V559fs.

(Varghese and Cohen, 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mir-14^Δ1 is rescued by mir-14^{UAS.cLa.DsRed(Unk)}/Scer\GAL4^btl.PU

(Kim et al., 2014)

mir-14^Δ1 is rescued by Scer\GAL4^fkh.PU/mir-14^UAS.pLUC

(Nelson et al., 2014)

mir-14^Δ1 is rescued by mir-14^{UAS.DsRed(Unk)}/Scer\GAL4^arm.PS

(Varghese et al., 2010)

mir-14^Δ1 is rescued by mir-14^{UAS.DsRed(Unk)}/Scer\GAL4^Ilp2.215-3

(Varghese et al., 2010)

mir-14^Δ1 is rescued by mir-14^+t3.4

(Xu et al., 2003)

Not rescued by

mir-14^Δ1 is not rescued by mir-14^{UAS.DsRed(Unk)}/Scer\GAL4^Lsp2.PH

(Varghese et al., 2010)

Comments

Expression of mir-14^{Scer\UAS.cLa.T:Disc\RFP} driven by Scer\GAL4^btl.PU rescues the excess tracheal terminal cell phenotype seen in mir-14^Δ1/mir-14^Δ1 third instar larvae.

(Kim et al., 2014)

Expression of mir-14^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^fkh.PU rescues the persistent salivary gland phenotype seen in mir-14^Δ1 homozygotes 24 hours after pupariation.

(Nelson et al., 2014)

Expression of mir-14^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^Lsp2.PH does not rescue the elevated total body fat to total body protein ratio seen in mir-14^Δ1 adults.

Expression of mir-14^{Scer\UAS.T:Disc\RFP} under the control of either Scer\GAL4^Ilp2.215-3 or Scer\GAL4^arm.PS rescues the elevated total body fat to total body protein ratio seen in mir-14^Δ1 adults.

(Varghese et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33067

w^*; mir-14^Δ1/CyO

58895

w^*; mir-14^Δ1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

miR-14^-

(Varghese and Cohen, 2007)

miR-14^Δ1

(Luedke et al., 2024, Kim et al., 2014, Nelson et al., 2014)

miR-14^Δ

(Kim et al., 2014)

miR14^Δ1

(Bejarano et al., 2008)

mir-14 null

(McNeill et al., 2020)

mir-14^Δ1

mir-14Δ¹

(Xu et al., 2003)

ΔmiR-14

(Mugat et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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