FlyBase Allele Report: Dmel\Arpc1[Q25st]

General Information

Symbol

Dmel\Arpc1^Q25st

Species

D. melanogaster

Name

FlyBase ID

FBal0150605

Feature type

allele

Associated gene

Dmel\Arpc1

Associated Insertion(s)

Carried in Construct

Also Known As

sop2^Q25st

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hudson and Cooley, 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q25term.

(Hudson and Cooley, 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:13,829,346..13,829,346 [-]

Nucleotide change:

C13829346T

Reported nucleotide change:

C?T

Amino acid change:

Q25term | Arpc1-PA; Q25term | Arpc1-PB

Reported amino acid change:

Q25term

(Hudson and Cooley, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Gokhale et al., 2016)

lethal (with Arpc1¹)

(Hudson and Cooley, 2002)

lethal (with Df(2L)b84a9)

(Hudson and Cooley, 2002)

lethal | recessive

(Hudson and Cooley, 2002)

visible

(Gohl et al., 2010)

Phenotype Manifest In

anterior dorsocentral bristle | somatic clone

(Hudson and Cooley, 2002)

external sensory organ precursor cell IIa | somatic clone

(Rajan et al., 2009)

external sensory organ precursor cell IIb | somatic clone

(Rajan et al., 2009)

follicle cell | somatic clone

(Sarpal et al., 2012)

macrochaeta | somatic clone

(Hudson and Cooley, 2002)

mechanosensory chaeta | somatic clone

(Rajan et al., 2009)

myoblast | embryonic stage

(Massarwa et al., 2007)

NMJ bouton | increased number | third instar larval stage

(Gokhale et al., 2016)

nurse cell & actin filament | germ-line clone

(Hudson and Cooley, 2002)

nurse cell | germline clone

(Hudson and Cooley, 2002)

nurse cell ring canal | germline clone

(Hudson and Cooley, 2002)

oocyte | germline clone

(Hudson and Cooley, 2002)

wing

(Gohl et al., 2010)

Detailed Description

Statement

Reference

Arpc1^Q25st heterozygous mutant third instar larvae display increased number of boutons on neuromuscular junctions.

(Gokhale et al., 2016)

Arpc1^Q25st/+ mutants embryos show no defects in development or survival.

Arpc1^Q25st mutant follicle cell clones are apically constricted, and adherens junctions appear jagged between mutant cells and between mutant and wild type cells. The phenotypes are most pronounced in stage 11 or older egg chambers.

(Sarpal et al., 2012)

Arpc1^Q25st mutant wings frequently show wing blisters and the morphology of the wings is grossly affected.

(Gohl et al., 2010)

Arpc1^Q25st clones in the adult thorax show bristle loss.

Within Arpc1^Q25st pIIa-pIIb cells, an apical actin-rich structure (ARS) is formed as in wild type, but its apical area is markedly reduced and, in about 50% of cases, the stalk of the normal 'umbrella shape' is not formed properly.

(Rajan et al., 2009)

Embryos homozygous for Sop2^Q25st commonly display unfused myoblasts.

(Massarwa et al., 2007)

Single cell γ neuron mutant clones in the mushroom body do not show axon growth defects.

(Ng and Luo, 2004)

Germ-line clones of Sop2^Q25st lead to defects in nurse cell dumping: late stage oocytes are abnormally small, while late stage nurse cells are abnormally large. By stage 10B the arrangement of actin filament bundles in the cytoplasm of mutant nurse cells is abnormally uneven, and the diameter and shape of ring canals between these cells is severely compromised. There are approximately twice as many longitudinal ridges on anterior dorso-central bristles in Sop2^Q25st mutant clones as in wild-type. A similar phenotype is seen in all other mutant macrochaete.

(Hudson and Cooley, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Arpc1^Q25st has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Blos1[+]/Blos1^ex65

(Gokhale et al., 2016)

Suppressor of

Statement

Reference

Arpc1[+]/Arpc1^Q25st is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Blos1^ex65

(Gokhale et al., 2016)

Arpc1[+]/Arpc1^Q25st is a suppressor of abnormal cell migration phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

NOT Suppressor of

Statement

Reference

Arpc1[+]/Arpc1^Q25st is a non-suppressor of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Sop2[+]/Arpc1^Q25st is a non-suppressor of abnormal mitotic cell cycle | maternal effect phenotype of Abl⁴

(Grevengoed et al., 2003)

Other

Statement

Reference

Arpc1^Q25st, Csk^GD9345 has lethal phenotype

(Rudrapatna et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Arpc1^Q25st has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Blos1[+]/Blos1^ex65

(Gokhale et al., 2016)

Suppressor of

Statement

Reference

Arpc1[+]/Arpc1^Q25st is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Blos1^ex65

(Gokhale et al., 2016)

Arpc1[+]/Arpc1^Q25st is a suppressor of wing disc phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

Arpc1[+]/Arpc1^Q25st is a suppressor | partially of embryonic/larval cuticle | embryonic stage phenotype of α-Cat¹

(Sarpal et al., 2012)

NOT Suppressor of

Statement

Reference

Arpc1[+]/Arpc1^Q25st is a non-suppressor of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Arpc1[+]/Arpc1^Q25st is a non-suppressor of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Sop2[+]/Arpc1^Q25st is a non-suppressor of actin cytoskeleton | maternal effect | embryonic stage phenotype of Abl⁴

(Grevengoed et al., 2003)

Sop2[+]/Arpc1^Q25st is a non-suppressor of spindle | maternal effect | embryonic stage phenotype of Abl⁴

(Grevengoed et al., 2003)

Other

Statement

Reference

Arpc1^Q25st, wash^Δ185 has nurse cell phenotype

(Liu et al., 2009)

Arpc1^Q25st, RpII140[+]/Polr2B^wimp, wash^Δ185 has nurse cell phenotype

(Liu et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of boutons on neuromuscular junctions observed in third instar larvae heterozygous for either Arpc1^Q25st or Blos1^ex65 is restored to wild-type levels in Arpc1^Q25st/+;Blos1^ex65/+ double heterozygotes.

(Gokhale et al., 2016)

Arpc1^Q25st/+ fails to suppress the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

(Flores-Benitez and Knust, 2015)

An Arpc1^Q25st heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing Csk^GD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4^ptc-559.1.

(Rudrapatna et al., 2014)

One copy of Arpc1^Q25st partially suppresses the cuticle defects seen in α-Cat¹ mutant embryos. The phenotype is more severe when Arpc1^Q25st is introduced maternally rather than paternally.

(Sarpal et al., 2012)

wash^Δ185/+ ; Sop2^Q25st/+ egg chambers show a loss of nurse cell integrity at stage 10a and incomplete stress fiber formation during during nurse cell dumping (stages 10b-11) compared to wild type. The actin network around the ring canals is abnormal by stage 10b.

wash^Δ185/+ ; RpII140^wimp/+ Sop2^Q25st egg chambers show a loss of nurse cell integrity at stage 10a and incomplete stress fiber formation during during nurse cell dumping (stages 10b-11) compared to wild type. The actin network around the ring canals is abnormal by stage 10b.

(Liu et al., 2009)

Heterozygosity for Sop2^Q25st does not suppress the Abl⁴ phenotype.

(Grevengoed et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Arpc1^Q25st is rescued by Arpc1^+tPa

(Hudson and Cooley, 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9135

P{hsFLP}12, y¹ w^*; nub¹ Arpc1^Q25st P{neoFRT}40A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Arpc1^Q25St

(Sarpal et al., 2012)

Arpc1^Q25st

(Gokhale et al., 2016, Flores-Benitez and Knust, 2015, Rajan et al., 2009, Massarwa et al., 2007, Hudson and Cooley, 2002)

Sop2^Q25st

(Sarpal et al., 2012, Liu et al., 2009)

arpc1^Q25St

(Herszterg et al., 2013)

arpc1^Q25st

(Yaniv et al., 2020)

sop2^Q25st

(Rudrapatna et al., 2014, Gohl et al., 2010, Grevengoed et al., 2003)

sop^Q25st

(Gohl et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (13)

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