Nonsense mutation which causes a premature truncation of the Mmp1 hemopexin domain in the third of the 4 hemopexin loops.
Amino acid replacement: W439term.
G24675316A
W439term | Mmp1-PC; W439term | Mmp1-PD; W439term | Mmp1-PE; W439term | Mmp1-PF; W439term | Mmp1-PG; W439term | Mmp1-PH; W439term | Mmp1-PI; W439term | Mmp1-PJ; W439term | Mmp1-PK; W439term | Mmp1-PL
W439term
G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change. The current annotation uses an upstream AUG 29aa upstream relative to previous reports. This accounts for the discrepency in the reported vs. annotated amino acid coordinate of the mutation.
decreased rate of movement | larval stage (with Mmp1Q273stop), with Mmp1E225A.f1.UAS
lethal | pupal stage (with Mmp12)
head | pupal stage (with Mmp1Q273stop)
head | pupal stage (with Mmp1Q273stop), with Mmp1E225A.f1.UAS, Scer\GAL4αTub84B.PL
head | pupal stage (with Mmp1Q273stop), with Mmp1Pro-pex.f1.UAS, Scer\GAL4αTub84B.PL
wing | pupal stage (with Mmp1Q273stop)
wing | pupal stage (with Mmp1Q273stop), with Mmp1E225A.f1.UAS, Scer\GAL4αTub84B.PL
wing | pupal stage (with Mmp1Q273stop), with Mmp1Pro-pex.f1.UAS, Scer\GAL4αTub84B.PL
7% of Mmp1W439stop mutant third instar larvae display broken tracheal dorsal trunks. 23% survive to pupariation. Dissected pupae display wing and head eversion defects.
6% of Mmp1Q273stop/Mmp1W439stop transheterozygous mutant third instar larvae display broken tracheal dorsal trunks. 78% survive to pupariation. A small number of dissected pupae show defects in head eversion.
Very few Mmp1Q273stop/Mmp1W439stop transheterozygous mutant third instar larvae expressing Mmp1Pro-pex.f1.Scer\UAS under the control of Scer\GAL4αTub84B.PL display broken tracheal dorsal trunks. The majority of dissected pupae show defects in head eversion, and some animals show additional defects in wing eversion.
Expression of Mmp1E225A.f1.Scer\UAS under the control of Scer\GAL4αTub84B.PL strongly enhances the phenotype of Mmp1Q273stop/Mmp1W439stop transheterozygous mutant third instar larvae. 15% display broken tracheal dorsal trunks and appear sluggish, and the majority of dissected pupae show defects in wing and head eversion. These phenotypes are stronger than either Mmp1Q273stop/Mmp1W439stop or Scer\GAL4αTub84B.PL driving Mmp1E225A.f1.Scer\UAS alone.
73% of Mmp1W439stop mutant animals survive to 3rd instar, 32% pupariate, but only 2% undergo head eversion, and none eclose. 90% of Mmp1W439stop/Mmp12 animals survive to third instar, but only 10% pupariate, 2% undergo head eversion, and none eclose.