FlyBase Allele Report: Dmel\Mmp1[2]

General Information

Symbol

Dmel\Mmp1²

Species

D. melanogaster

Name

FlyBase ID

FBal0150761

Feature type

allele

Associated gene

Dmel\Mmp1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Page-McCaw et al., 2003)

Progenitor genotype

Mmp1^k04809

(Page-McCaw et al., 2003)

Cytology

Description

A 3.2Kb deletion caused by the imprecise excision of P{lacW}Mmp1^k04809. The deletion starts at the P{lacW}Mmp1^k04809 insertions site in the second intron of Mmp1 and removes exons 3,4 and 5.

(Page-McCaw et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage (with Mmp1^Q112stop)

(Miller et al., 2008)

abnormal size (with Mmp1^Q112stop)

(Glasheen et al., 2010)

abnormal size | late third instar larval stage

(Glasheen et al., 2010)

abnormal size | second instar larval stage

(Glasheen et al., 2010)

decreased body size | larval stage (with Mmp1^Q112stop)

(Page-McCaw et al., 2003)

lethal | pupal stage (with Mmp1^Q112stop)

(Page-McCaw et al., 2003)

lethal | pupal stage (with Mmp1^Q273stop)

(Page-McCaw et al., 2003)

lethal | pupal stage (with Mmp1^W208stop)

(Page-McCaw et al., 2003)

lethal | pupal stage (with Mmp1^W439stop)

(Page-McCaw et al., 2003)

lethal - all die before end of larval stage

(Glasheen et al., 2010, Glasheen et al., 2009)

lethal - all die before end of larval stage (with Mmp1^Q112stop)

(Glasheen et al., 2009)

partially lethal - majority die | larval stage (with Mmp1^Q112stop)

(Page-McCaw et al., 2003)

partially lethal - majority die | larval stage (with Mmp1^Q273stop)

(Page-McCaw et al., 2003)

partially lethal - majority die | larval stage (with Mmp1^W208stop)

(Page-McCaw et al., 2003)

partially lethal - majority die | larval stage (with Mmp1^W439stop)

(Page-McCaw et al., 2003)

some die during larval stage

(Glasheen et al., 2010, Glasheen et al., 2009)

some die during larval stage (with Mmp1^Q112stop)

(Glasheen et al., 2009)

Phenotype Manifest In

embryonic/larval dorsal tracheal branch | third instar larval stage

(Glasheen et al., 2009)

embryonic/larval dorsal tracheal branch | third instar larval stage (with Mmp1^Q112stop)

(Glasheen et al., 2009)

embryonic/larval posterior spiracle

(Page-McCaw et al., 2003)

embryonic/larval tracheal dorsal trunk

(Glasheen et al., 2010, Page-McCaw et al., 2003)

embryonic/larval tracheal dorsal trunk (with Mmp1^Q112stop)

(Page-McCaw et al., 2003)

embryonic/larval tracheal section (with Mmp1^Q112stop)

(Page-McCaw et al., 2003)

embryonic/larval tracheal transverse connective (with Mmp1^Q112stop)

(Page-McCaw et al., 2003)

larval intersegmental nerve branch ISNb of A1-7 (with Mmp1^Q112stop)

(Miller et al., 2008)

larval segmental nerve branch SNa of A1-7 (with Mmp1^Q112stop)

(Miller et al., 2008)

second instar larval cuticle

(Page-McCaw et al., 2003)

taenidium | larval stage

(Glasheen et al., 2010)

taenidium | larval stage (with Mmp1^Q112stop)

(Glasheen et al., 2010)

Detailed Description

Statement

Reference

At the beginning of the third instar taenidial spacing in Mmp1² larvae does not differ from wild type.

Intertaenidial distance does not increase from early to late third instar in Mmp1² mutants whereas an approximately two fold increase in elongation occurs in controls. The lack of intertaenidial elongation compared to body length increase in Mmp1² mutant results in broken dorsal trunk trachea in 93% of third instar larvae.

Mmp1²/Mmp1^Q112stop mutants display similar taenidial spacing at the early third instar stage compared to controls but reduced intertaenidial distance compared to wild type by late third instar.

Mmp1² mutants have a reduced angle between the dorsal trunk and the transverse connective compared to wild type.

The intertaenidial distance in Mmp1² early second instar larvae is similar to wild type but the elongation that occurs between the early and late second instar in wild type does not take place in Mmp1² mutants.

The shedding of cuticle in the dorsal trunk that takes place in wild type prior to the third instar stage does not take place in a large proportion of Mmp1² mutants, with many larvae having two or three cuticles compared to one in controls.

When the inner cuticle breaks in wild type the break occurs in the vicinity of the fusion cells and disrupts their pattern, whereas 80% of the breaks seen in Mmp1² mutants are not in the vicinity of the fusion cells and the pattern is intact.

A separation between the cell and cuticular layers is seen in late third instar Mmp1² mutants that is not seen in wild type or in early third instar mutant larvae.

The ratio of apical membrane length to tracheal tube length is greater in Mmp1² late third instar larvae compared to controls indicating that the membrane elongates more than the matrix. However the length of the apical membrane is still reduced in Mmp1² mutants compared to wild type.

(Glasheen et al., 2010)

45% of Mmp1² mutant third instar larvae display broken tracheal dorsal trunks. None survive to pupariation.

44% of Mmp1^Q112stop/Mmp1² transheterozygous mutant third instar larvae display broken tracheal dorsal trunks.

(Glasheen et al., 2009)

ISNb pathfinding in Mmp1^Q112stop/Mmp1² embryos is roughly wild type, though 28% of hemisegments display a loosely fasciculated ISNb morphology. SNa fasciculation is also decreased.

(Miller et al., 2008)

90% of Mmp1^W439stop/Mmp1² animals survive to third instar, but only 10% pupariate, 2% undergo head eversion, and none eclose. 87% of Mmp1^Q273stop/Mmp1² animals survive to third instar, but only 22% pupariate, 6% undergo head eversion, and none eclose. Most Mmp1^W208stop/Mmp1² animals survive to third instar (90%), but only 6% pupariate; 4% undergo head eversion, but none eclose. Most Mmp1^Q112stop/Mmp1² animals survive to third instar (84%), but only 7% pupariate. All 7% undergo head eversion, but none eclose. Third instar Mmp1² mutant larvae frequently have breaks in the dorsal trunk and internalised posterior spiracles. The tracheal system phenotypes of Mmp1^Q112stop/Mmp1² larvae get more severe with age: 1. First instar: in small first instars larval trachea do not have quite as much slack as those of controls. This defect is more apparent in larger first instar larvae, where the tracheal system is visibly stretched and has no slack, especially along the transverse connectives. 20% (n = 25) of have breaks in their dorsal trunks, presumably caused by overstretching. 2. Second instar: shortened dorsal trunks; the posterior spiracles have moved inward; 44% (n = 62) have visible breaks in the dorsal trunks. 3. Third instar: The tracheal system is stretched dramatically toward the anterior of the animal, with prominent tracheal breaks in 42% (n = 52) and posterior spiracles separated from the surface of the cuticle in all. Mmp1^Q112stop/Mmp1² larvae are variable in size, but are always small than their wild-type siblings. Occasional Mmp1² mutant animals fail to shed their cuticles completely at the L2/L3 molt: The old mutant cuticle appears to remain attached at the mouthhooks and/or at a lateral spiracle through which tracheal cuticle is shed.

(Page-McCaw et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mmp1^Q112stop/Mmp1² has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by Mmp2^W307stop/Df(2R)Uba1-Mmp2

(Miller et al., 2008)

Mmp1^Q112stop/Mmp1² has partially lethal - majority die | larval stage phenotype, enhanceable by Mmp2^W307stop/Mmp2²¹⁸

(Page-McCaw et al., 2003)

Suppressed by

Statement

Reference

Mmp1² has lethal | larval stage phenotype, suppressible by Tb[+]/Tb¹

(Glasheen et al., 2010)

Mmp1^Q112stop/Mmp1² has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2008)

NOT suppressed by

Statement

Reference

Mmp1² has abnormal size | late third instar larval stage phenotype, non-suppressible by Tb[+]/Tb¹

(Glasheen et al., 2010)

Mmp1^Q112stop/Mmp1² has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2008)

Enhancer of

Statement

Reference

Mmp1^Q112stop/Mmp1² is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Mmp2^W307stop/Df(2R)Uba1-Mmp2

(Miller et al., 2008)

NOT Enhancer of

Statement

Reference

Mmp1^Q112stop/Mmp1² is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of Mmp2^W307stop/Df(2R)Uba1-Mmp2

(Miller et al., 2008)

Suppressor of

Statement

Reference

Mmp1[+]/Mmp1² is a suppressor | partially of visible | adult stage phenotype of Mnat9^KK109382, Scer\GAL4^bbg-C96

(Mok and Choi, 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mmp1^Q112stop/Mmp1² has larval segmental nerve branch SNa of A1-7 phenotype, enhanceable by Mmp2^W307stop/Df(2R)Uba1-Mmp2

(Miller et al., 2008)

Suppressed by

Statement

Reference

Mmp1² has embryonic/larval tracheal dorsal trunk phenotype, suppressible by Tb[+]/Tb¹

(Glasheen et al., 2010)

Mmp1^Q112stop/Mmp1² has larval segmental nerve branch SNa of A1-7 phenotype, suppressible by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2008)

NOT suppressed by

Statement

Reference

Mmp1² has taenidium | larval stage phenotype, non-suppressible by Tb[+]/Tb¹

(Glasheen et al., 2010)

Mmp1^Q112stop/Mmp1² has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-suppressible by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2008)

Enhancer of

Statement

Reference

Mmp1^Q112stop/Mmp1² is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of Mmp2^W307stop/Df(2R)Uba1-Mmp2

(Miller et al., 2008)

NOT Enhancer of

Statement

Reference

Mmp1^Q112stop/Mmp1² is a non-enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of Mmp2^W307stop/Df(2R)Uba1-Mmp2

(Miller et al., 2008)

Suppressor of

Statement

Reference

Mmp1[+]/Mmp1² is a suppressor | partially of wing phenotype of Mnat9^KK109382, Scer\GAL4^bbg-C96

(Mok and Choi, 2021)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Tb¹ in a Mmp1² mutant background partially rescues the tracheal break phenotype seen in Mmp1² homozygotes (55% of larvae have tracheal breaks in the dorsal trunk compared to 93%).

The angle between the transverse connective and the dorsal trunk is reduced in Tb¹ Mmp1² double mutants compared to homozygous Mmp1² mutants but is still greater than in wild type.

No homozygous Mmp1² larvae survive to pupariation, whereas 29% of Tb¹ Mmp1² double mutants survive beyond the larval stages.

The failure of the taenidial distance to increase from the early to late third instar stages that is seen in Mmp1² mutants is still present in flies that also carry one copy of Tb¹.

(Glasheen et al., 2010)

ISNb axon guidance defects in Mmp2^W307stop Mmp1^Q112stop or Mmp2^W307stop/Df(2R)Uba1-Mmp2 Mmp1^Q112stop/Mmp1² double mutants qualitatively and quantitatively mirror the phenotypes observed in the Mmp2 single mutants.

SNa axon defasciculation in Mmp2^W307stop Mmp1^Q112stop or Mmp2^W307stop/Df(2R)Uba1-Mmp2 Mmp1^Q112stop/Mmp1² double mutants is significantly increased relative to that of either single mutant.

(Miller et al., 2008)

The larval lethality due to Mmp1^Q112stop/Mmp1² is enhanced by Mmp2²¹⁸/Mmp2^W307stop from 84% survival to 3rd instar and 7% pupariating to 54% survival to 3rd instar and 4% pupariating.

(Page-McCaw et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58709

w^*; Mmp1²/CyO

Notes on Origin

Discoverer

Comments

Null allele

(Glasheen et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

MMP1²

(Matsubayashi et al., 2020)

Mmp1²

(Glasheen et al., 2010, Yasunaga et al., 2010, Glasheen et al., 2009, McClure et al., 2008, Miller et al., 2008)

mmp1²

(Calderon et al., 2022, Sauerwald et al., 2019, Raza et al., 2017, Dear et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (12)

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