FB2024_03 , released June 25, 2024
Allele: Dmel\ppk11RNAi.UAS
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General Information
Symbol
Dmel\ppk11RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0151633
Feature type
allele
Associated gene
Dmel\ppk11
Associated Insertion(s)
Carried in Construct
P{UAS-ppk11.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Liu et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Liu et al., 2003)
Mutagen
in vitro construct
(Liu et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-ppk11.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of 500bp of antisense sequence followed by 500bp of sense sequence to the ppk11 N-terminal portion of the cDNA.

(Liu et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
ppk11
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Third instar larvae expressing ppk11dsRNA.Scer\UAS under the control of Scer\GAL4VGlut-OK371 show no significant increase in quantal content at the neuromuscular junction in the presence of the glutamate receptor antagonist philanthotoxin-433 (10μM), indicating a defect in synaptic homeostasis. In the absence of philanthotoxin-433, the larvae show no significant change in evoked excitatory postsynaptic potential (EPSP) amplitude, miniature mEPSP amplitude or quantal content compared to controls.

      Third instar larvae expressing ppk11dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW do not show defects in synaptic homeostasis in the presence of philanthotoxin-433.

      (Younger et al., 2013)

      Larvae expressing ppk11dsRNA.Scer\UAS under the control of Scer\GAL4elav.PLu or Scer\GAL4hs.PB show defective salt-taste behaviour (a reduced preference for 10mM NaCl). The terminal organ of larvae expressing ppk11dsRNA.Scer\UAS under the control of Scer\GAL4elav.PLu shows a reduction in the frequency of action potentials produced in response to salt compared to wild type.

      (Liu et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Transcription from the P{UAS-ppk11.dsRNA} construct should produce ppk11 dsRNA, resulting in dsRNA interference (RNAi) of the ppk11 gene.

      (Liu et al., 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ppk11RNAi.UAS
      ppk11dsRNA.Scer\UAS
      ppk11dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)