UASt regulatory sequences drive expression of a cDNA encoding a mutated form of Hsap\FOXO3; carries mutations in all three PKB consensus phosphorylation sites.
When expression is driven by Scer\GAL4GMR.PF at 18oC adult escapers of the pupal lethality show a necrotic eye phenotype and disruption of cell specification. In somatic clones in the eye a block of cell differentiation and necrosis is evident.