FlyBase Allele Report: Dmel\Scm[SPM.UAS.Tag:HA]

General Information

Symbol

Dmel\Scm^{SPM.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0159771

Feature type

allele

Associated gene

Dmel\Scm

Associated Insertion(s)

Carried in Construct

P{UAS-Scm.SPM}

Key Links

Transgenic product class

inframe_deletion

(Peterson et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Peterson et al., 2004)

Mutagen

in vitro construct

(Peterson et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Scm.SPM}

Cytology

Description

UASt regulatory sequences drive expression of the SPM domain of Scm (the C-terminal 77 amino acids) which is tagged at the N-terminal end with Tag:HA and a nuclear localisation signal.

(Peterson et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^dpp.blk1, Scm^H1

(Peterson et al., 2004)

lethal | male, with Scer\GAL4^prd.RG1

(Peterson et al., 2004)

lethal - all die during embryonic stage | heat sensitive, with Scer\GAL4^da.G32

(Peterson et al., 2004)

some die during embryonic stage | heat sensitive, with Scer\GAL4^da.G32

(Peterson et al., 2004)

viable, with Scer\GAL4^71B

(Peterson et al., 2004)

viable, with Scer\GAL4^A9

(Peterson et al., 2004)

viable, with Scer\GAL4^ap-md52

(Peterson et al., 2004)

viable | female, with Scer\GAL4^prd.RG1

(Peterson et al., 2004)

visible, with Scer\GAL4^71B

(Peterson et al., 2004)

visible, with Scer\GAL4^A9

(Peterson et al., 2004)

visible, with Scer\GAL4^A9, Scm^H1

(Peterson et al., 2004)

visible, with Scer\GAL4^ap-md52

(Peterson et al., 2004)

visible | female | homeotic, with Scer\GAL4^prd.RG1

(Peterson et al., 2004)

visible | homeotic | male, with Scer\GAL4^dpp.blk1

(Peterson et al., 2004)

visible | homeotic | male, with Scer\GAL4^ptc-559.1

(Peterson et al., 2004)

Phenotype Manifest In

antenna | female, with Scer\GAL4^prd.RG1

(Peterson et al., 2004)

haltere | ectopic, with Scer\GAL4^ap-md52

(Peterson et al., 2004)

leg | ectopic | female, with Scer\GAL4^prd.RG1

(Peterson et al., 2004)

leg | male, with Scer\GAL4^dpp.blk1

(Peterson et al., 2004)

leg | male, with Scer\GAL4^ptc-559.1

(Peterson et al., 2004)

sex comb | ectopic, with Scer\GAL4^dpp.blk1

(Peterson et al., 2004)

sex comb | ectopic, with Scer\GAL4^ptc-559.1

(Peterson et al., 2004)

wing, with Scer\GAL4^71B

(Peterson et al., 2004)

wing, with Scer\GAL4^A9

(Peterson et al., 2004)

wing, with Scer\GAL4^A9, Scm^H1

(Peterson et al., 2004)

wing, with Scer\GAL4^ap-md52

(Peterson et al., 2004)

Detailed Description

Statement

Reference

Expression of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^71B results in mild crumpling of the wing. Expression of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 results in dramatically reduced wings. The reduced wing phenotype seen in animals expressing Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 is substantially suppressed by coexpression of Scm^{Scer\UAS.T:Ivir\HA1}. The penetrance of severe wing types seen in animals expressing Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 is enhanced in a Scm^H1/+ background. Expression of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ap-md52 results in dramatically reduced wings which resemble halteres. Expression of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^dpp.blk1 results in male progeny with an extra sex combs phenotype. Expression of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^dpp.blk1 in a Scm^H1/+ background results in lethality. Expression of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^prd.RG1 produces partial antenna-to-leg transformations in females (males expressing Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^prd.RG1 do not survive to adulthood). Expression of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ptc-559.1 results in an extra sex comb phenotype in pharate adult males. Embryos expressing one or two copies of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of one copy of Scer\GAL4^da.G32 are completely viable at 25^oC. At 29^oC, expression of one copy of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of one copy of Scer\GAL4^da.G32 reduces embryonic viability to 51%, and expression of two copies of Scm^{SPM.Scer\UAS.T:Ivir\HA1} under the control of one copy of Scer\GAL4^da.G32 results in complete embryonic lethality (less than 1% of embryos are viable).

(Peterson et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scm^{SPM.Scer\UAS.T:Ivir\HA1}

Scm^{SPM.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (2)

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