FlyBase Allele Report: Dmel\mld[47]

General Information

Symbol

Dmel\mld⁴⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0178854

Feature type

allele

Associated gene

Dmel\mld

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neubueser et al., 2005)

Progenitor genotype

P{EP}30221

(Neubueser et al., 2005)

Cytology

Description

Amino acid replacement: Q258term.

(Neubueser et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:24,616,171..24,616,171 [+]

Nucleotide change:

C24616171T

Amino acid change:

Reported amino acid change:

Q258term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Neubueser et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with Df(3R)crb87-5)

(Neubueser et al., 2005)

lethal - all die before end of first instar larval stage (with mld⁴⁴²⁵)

(Ono et al., 2006)

lethal - all die before end of larval stage (with Df(3R)crb87-5)

(Neubueser et al., 2005)

some die during first instar larval stage (with mld⁴⁴²⁵)

(Ono et al., 2006)

some die during larval stage (with Df(3R)crb87-5)

(Neubueser et al., 2005)

Phenotype Manifest In

ring gland (with Df(3R)crb87-5)

(Neubueser et al., 2005)

Detailed Description

Statement

Reference

mld⁴⁷/mld⁴⁴²⁵ mutants die after a prolonged first instar stage without molting.

(Ono et al., 2006)

mld⁴⁷/Df(3R)crb87-5 mutants appear to develop normally up to the first larval stage. However, these animals fail to complete the first larval molt. Instead of molting, the mutant larvae continue to grow to the size of second instar larvae and contain a well-developed fat body, a structure not normally visible in first instar larvae, indicating that the larvae continue to feed. These larvae have enlarged ecdysone-producing gland cells, that in turn contain enlarged nuclei. The majority of mld⁴⁷/Df(3R)crb87-5 larvae die at 3-5 days of age. Levels of ecdysteroid production are reduced in mld⁴⁷/Df(3R)crb87-5 mutants; addition of these substances partially rescues the mld⁴⁷/Df(3R)crb87-5 phenotype.

(Neubueser et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

mld⁴⁴²⁵/mld⁴⁷ has lethal | first instar larval stage phenotype, non-suppressible by spo^UAS.cOa/Scer\GAL4^phtm.PO

(Ono et al., 2006)

mld⁴⁴²⁵/mld⁴⁷ has lethal | first instar larval stage phenotype, non-suppressible by Scer\GAL4^phtm.PO/spok^UAS.cOa