FB2024_03 , released June 25, 2024
Allele: Dmel\MbsT666
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General Information
Symbol
Dmel\MbsT666
Species
D. melanogaster
Name
FlyBase ID
FBal0182788
Feature type
allele
Associated gene
Dmel\Mbs
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Lee and Treisman, 2004)
Mutagen
ethyl methanesulfonate
(Lee and Treisman, 2004)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q239term.

(Lee and Treisman, 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:16,062,651..16,062,651 [+]
Nucleotide change:

C16062651T

Amino acid change:

Q238term | Mbs-PG; Q238term | Mbs-PH; Q238term | Mbs-PI; Q238term | Mbs-PJ; Q238term | Mbs-PK; Q238term | Mbs-PL; Q238term | Mbs-PM; Q238term | Mbs-PN; Q238term | Mbs-PO; Q238term | Mbs-PP; Q238term | Mbs-PQ; Q238term | Mbs-PV

Reported amino acid change:

Q239term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Lee and Treisman, 2004)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Egg chambers containing follicle cells mutant for MbsT666 display oocyte polarity defects.

      (Sun et al., 2011)

      Transheterozygous Mbs03802/MbsT666 mutants exhibit small ring canals.

      (Ong et al., 2010)

      MbsT666 mutant clones in the eye disc show a mislocalization of photoreceptors from the apical epithelium into the basal region, sometimes progressing into the optic stalk. The mislocalized photoreceptors retain their polarity and some epithelial characteristics. There is only a slight reduction in apical constriction in the morphogenetic furrow in MbsT666 clones and no ectopic apical constriction in more posterior regions of the disc. MbsT666 mutant clones in adult wings have short wing hairs that are sometimes forked. Unlike the situation in eye discs, wing disc cells are not mislocalized basally in MbsT666 clones.

      (Lee and Treisman, 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of either constitutively inactive MoeT559A.Scer\UAS.T:Hsap\MYC transgene, or the phosphomimetic MoeT559D.Scer\UAS.T:Hsap\MYC transgene, both under the control of Scer\GAL4tub, does not affect the MbsT666 mutant photoreceptor localization phenotype.

      (Lee and Treisman, 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      MbsT111

      (Lee and Treisman, 2004)

      MbsT541

      (Lee and Treisman, 2004)

      MbsT791

      (Lee and Treisman, 2004)

      MbsS041315a

      (Lee and Treisman, 2004)

      MbsS095304

      (Lee and Treisman, 2004)

      Mbs03802

      (Lee and Treisman, 2004)
      Partially rescued by

      MbsT541/MbsT666 is partially rescued by Scer\GAL4da.G32/MbsUAS.cLa

      (Lee and Treisman, 2004)

      MbsT666 is partially rescued by Scer\GAL4Tub.PU/MbsUAS.cLa

      (Lee and Treisman, 2004)
      Comments

      Expression of MbsScer\UAS.cLa, driven by Scer\GAL4da.G32, partially rescues the embryonic lethality of MbsT541/MbsT666 transheterozygotes, allowing 80% of these mutants to survive to the pupal stage, but only 6% to survive to adulthood. MbsT666 mutant eye disc clones that express MbsScer\UAS.cLa under the control of Scer\GAL4tub (generated using the MARCM technique) show normal apical localization within the eye disc, thus rescuing the eye development phenotype of the MbsT666 clones.

      (Lee and Treisman, 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Selected as: a mutation affecting early eye development.

      (Lee and Treisman, 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      MbsT666
      (Sun et al., 2011, Ong et al., 2010, Mitonaka et al., 2007)
      mbsT666
      (Widmann and Dahmann, 2009)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)