FlyBase Allele Report: Dmel\cv-c[7]

General Information

Symbol

Dmel\cv-c⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0189642

Feature type

allele

Associated gene

Dmel\cv-c

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Denholm et al., 2005)

Mutagen

ethyl methanesulfonate

(Denholm et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: R601Q. This mutation is located within the active site of the GTPase.

(Denholm et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:14,394,308..14,394,308 [-]

Nucleotide change:

G14394308A

Amino acid change:

R601Q | cv-c-PA; R1935Q | cv-c-PC; R1935Q | cv-c-PD

Reported amino acid change:

R601Q

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Denholm et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Denholm et al., 2005)

abnormal size | late embryonic stage (with cv-c^M62)

(Sotillos et al., 2018)

lethal - all die during embryonic stage

(Denholm et al., 2005)

some die during embryonic stage

(Denholm et al., 2005)

Phenotype Manifest In

cortical actin cytoskeleton & embryonic posterior spiracle

(Simões et al., 2006)

dorsal tracheal branch primordium

(Cela and Llimargas, 2006)

embryo | dorsal closure stage

(Denholm et al., 2005)

embryonic/first instar larval cuticle | dorsal

(Denholm et al., 2005)

embryonic/larval spiracle primordium

(Lovegrove et al., 2006)

embryonic head

(Denholm et al., 2005)

embryonic Malpighian tubule | late embryonic stage (with cv-c^M62)

(Sotillos et al., 2018)

embryonic trachea & cortical actin cytoskeleton

(Cela and Llimargas, 2006)

posterior spiracle primordium

(Simões et al., 2006)

presumptive embryonic/larval tracheal system

(Brodu and Casanova, 2006)

tracheal primordium

(Brodu and Casanova, 2006)

Detailed Description

Statement

Reference

In most cv-c⁷/cv-c^M62 (but not cv-c⁷/+) embryos Malpighian tubules fail to elongate.

(Sotillos et al., 2018)

cv-c⁷ mutant embryos exhibit a variably penetrant tracheal phenotype. Approximately 74% of the placodes show an abnormal invagination, without proper apical constriction. In these mutants, dorsal cells do not rotate, but instead the tracheal cells remain in a broad cavity. Myosin distribution is severely disrupted in cv-c⁷ mutant embryos. Instead of a well-shaped and uniform ring around the edge of the invagination site, there is a patchy distribution with the formation of myosin aggregates. Actin is as detected at the basal membrane in these mutants, as oppose to the apical wild-type situation. Additionally, the remaining apically located actin appears abnormal and seems to form aggregates.

(Brodu and Casanova, 2006)

cv-c⁷ embryos show a mild tracheal branch integrity phenotype that consists of cells that are abnormally separated from one another and are sometimes attached by cytoplasmic bridges. The accumulation of cortical actin is abnormal in the tracheal tubules.

(Cela and Llimargas, 2006)

30% of cv-c⁷ embryos show a spiracle invagination phenotype.

(Lovegrove et al., 2006)

Zygotic cv-c⁷ mutants show partial or complete invagination defects in their posterior spiracles and the apical actin pattern is strongly disorganised.

(Simões et al., 2006)

cv-c⁷ mutants display a strong head involution defect, in which the remnants of the whole head skeleton lie on the surface. In cv-c⁷ embryos, dorsal closure is delayed and puckering of the dorsal cuticle is frequently observed.

(Denholm et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

cv-c⁷/cv-c^M62 has abnormal size | late embryonic stage phenotype, suppressible | partially by Hsap\DLC1^UAS.Tag:MYC/Scer\GAL4^ct.CtB

(Sotillos et al., 2018)

cv-c⁷/cv-c^M62 has abnormal size | late embryonic stage phenotype, suppressible by Scer\GAL4^ct.CtB/cv-c::Hsap\DLC1^{dNCR.UAS.Tag:MYC}

(Sotillos et al., 2018)

cv-c⁷/cv-c^M62 has abnormal size | late embryonic stage phenotype, suppressible by Hsap\STARD8^{UAS.α.Tag:MYC}/Scer\GAL4^ct.CtB

(Sotillos et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

cv-c⁷/cv-c^M62 has embryonic Malpighian tubule | late embryonic stage phenotype, suppressible | partially by Hsap\DLC1^UAS.Tag:MYC/Scer\GAL4^ct.CtB

(Sotillos et al., 2018)

cv-c⁷/cv-c^M62 has embryonic Malpighian tubule | late embryonic stage phenotype, suppressible by Scer\GAL4^ct.CtB/cv-c::Hsap\DLC1^{dNCR.UAS.Tag:MYC}

(Sotillos et al., 2018)

cv-c⁷/cv-c^M62 has embryonic Malpighian tubule | late embryonic stage phenotype, suppressible by Hsap\STARD8^{UAS.α.Tag:MYC}/Scer\GAL4^ct.CtB

(Sotillos et al., 2018)

Enhancer of

Statement

Reference

cv-c[+]/cv-c⁷ is an enhancer of tracheal primordium phenotype of spi²

(Brodu and Casanova, 2006)

cv-c[+]/cv-c⁷ is an enhancer of presumptive embryonic/larval tracheal system phenotype of spi²

(Brodu and Casanova, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The mild invagination observed in spi² homozygous embryos is significantly enhanced by the presence of a single copy of cv-c⁷, with approximately 67% of placodes exhibiting an abnormal invagination, compared to approximately 35% in spi² mutants.

(Brodu and Casanova, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

cv-c⁷/cv-c^M62 is rescued by cv-c^{ΔSAMΔSTART.UAS.Venus}/Scer\GAL4^ct.CtB

(Sotillos et al., 2018)

Partially rescued by

cv-c⁷/cv-c^M62 is partially rescued by Scer\GAL4^ct.CtB/cv-c^UAS.C.Venus

(Sotillos et al., 2018)

cv-c⁷/cv-c^M62 is partially rescued by cv-c^{ΔSTART.UAS.Venus}/Scer\GAL4^ct.CtB

(Sotillos et al., 2018)

cv-c⁷/cv-c^M62 is partially rescued by Scer\GAL4^ct.CtB/cv-c^{Ct.UAS.Tag:PH(PLCδ-Unk).Venus}

(Sotillos et al., 2018)

Not rescued by

cv-c⁷/cv-c^M62 is not rescued by cv-c^Ct.UAS.Venus/Scer\GAL4^ct.CtB

(Sotillos et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63065

w^*; st¹ cv-c⁷ e¹/TM3, P{ActGFP}JMR2, Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

cv-c⁷

(Sotillos et al., 2022, Sotillos et al., 2018, Brodu and Casanova, 2006, Cela and Llimargas, 2006, Lovegrove et al., 2006, Simões et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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