FlyBase Allele Report: Dmel\Cks30A[KO]

General Information

Symbol

Dmel\Cks30A^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0189794

Feature type

allele

Associated gene

Dmel\Cks30A

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Swan et al., 2005)

gene targeting by homologous recombination

(Swan et al., 2005)

Progenitor genotype

Cytology

Description

The start codon of Cks30A has been removed.

(Swan et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Swan et al., 2005)

abnormal meiotic cell cycle | female

(Swan and Schupbach, 2007)

abnormal mitotic cell cycle | embryonic stage

(Swan et al., 2005)

lethal | maternal effect

(Swan et al., 2005)

male fertile

(Swan et al., 2005)

viable

(Swan et al., 2005)

Phenotype Manifest In

aster

(Swan et al., 2005)

larval cuticle & larval abdomen

(Swan et al., 2005)

larval cuticle & larval abdomen (with Cks30A^RA74)

(Swan et al., 2005)

spindle

(Swan et al., 2005)

spindle pole centrosome

(Swan et al., 2005)

Detailed Description

Statement

Reference

Eye imaginal discs from homozygous wandering third instar larvae have no detectable polyploidy.

(Ghorbani et al., 2011)

In 0-2 hour collections of Cks30A^KO/Cks30A^KO eggs, 26% are in meiosis II (indicating a delay in completing meiosis). In 58% of these, both spindles have a single X-chromosome signal, indicating that they are in metaphase of meiosis II, while the remaining 42% have two X-chromosome signals per spindle, indicating that they are in anaphase of meiosis II.

(Swan and Schupbach, 2007)

Cks30A^KO mutant embryos display a pronounced delay in entry into mitosis. In a 0-20 minute collection of embryos, only 29% have mitotic spindles, compared with 74% in wild-type. Instead they typically contain two separated microtubule asters, one of which is associated with condensed chromatin. In many cases, a thin spindle connects the two asters, suggesting that these are centrosomes that have duplicated in advance of mitosis but have not yet assembled a mitotic spindle. In 20-40 minute collections, 54% of embryos assemble a mitotic spindle, and in 40-80 minute collections, 80% of embryos exhibit a single mitotic spindle. The others appear to arrest with separated centrosomes that undergo a small number of extra divisions. Therefore Cks30A^KO mutants are delayed in, or arrest before, mitotic spindle assembly. Meiosis occurs normally in Cks30A^KO mutants up until the resumption of meiosis following egg activation, where the tandem meiosis II spindle is kinked or separated. In addition, Cks30A^KO mutants display a pronounced delay in metaphase of meiosis II. The delayed exit from female meiosis in Cks30A^KO embryos results in a failure to complete nuclear fusion. Cks30A^KO mutants exhibit defects in abdominal cuticle deposition, indicative of a partial disruption of larval abdominal histoblast development.

(Swan et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Cks30A^KO has viable phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Cks30A^KO has lethal | maternal effect phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Cks30A^KO has male fertile phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Cks30A^KO has abnormal meiotic cell cycle phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Cks30A^KO has abnormal mitotic cell cycle | embryonic stage phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Suppressor of

Statement

Reference

Cks30A^KO/Cks30A^KO is a suppressor of abnormal mitotic cell cycle | third instar larval stage phenotype of Cks85A^ex15

(Ghorbani et al., 2011)

Cks30A^KO/Cks30A^KO is a suppressor of visible phenotype of Scer\GAL4^en-e16E, cort^UASp.Tag:HA

(Swan and Schupbach, 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cks30A^KO/Cks30A^RA74 has larval cuticle & larval abdomen phenotype, suppressible | partially by CycA^C8LR1

(Swan et al., 2005)

NOT suppressed by

Statement

Reference

Cks30A^KO has aster phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Cks30A^KO has larval cuticle & larval abdomen phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Cks30A^KO has spindle pole centrosome phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Cks30A^KO has spindle phenotype, non-suppressible by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Suppressor of

Statement

Reference

Cks30A^KO/Cks30A^KO is a suppressor of eye disc | third instar larval stage phenotype of Cks85A^ex15

(Ghorbani et al., 2011)

Cks30A^KO/Cks30A^KO is a suppressor of wing blade posterior compartment phenotype of Scer\GAL4^en-e16E, cort^UASp.Tag:HA

(Swan and Schupbach, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The polyploidy seen in the eye discs of homozygous Cks85A^ex15 wandering third instar larvae anterior to the morphogenetic furrow is suppressed in Cks85A^ex15 Cks30A^KO double homozygotes.

(Ghorbani et al., 2011)

The increased spacing between the wing hairs that is seen in the posterior compartment of the wing in animals expressing cort^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E is suppressed if they are also homozygous for Cks30A^KO.

(Swan and Schupbach, 2007)

Expression of Cks85A^{Scer\UAS.T:Zzzz\FLAG}, under the control of Scer\GAL4^da.G32 does not suppress the Cks30A^KO phenotype. Reduction of CycA, in CycA^C8LR1 heterozygotes partially suppresses the Cks30A^KO/Cks30A^RA74 phenotype.

(Swan et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Not rescued by

Cks30A^KO is not rescued by Cks30A^{P61L.UAS.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Swan et al., 2005)

Cks30A^KO is not rescued by Scer\GAL4^{VP16.nanos.UTR}/Cks30A^{K10E.S50E.UAS.Tag:FLAG}

(Swan et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Cks30A^KO

(Das et al., 2016, Ghorbani et al., 2011, Swan and Schupbach, 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

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