FlyBase Allele Report: Dmel\pds5[e3]

General Information

Symbol

Dmel\pds5^e3

Species

D. melanogaster

Name

FlyBase ID

FBal0195337

Feature type

allele

Associated gene

Dmel\pds5

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Dorsett et al., 2005)

Progenitor genotype

pds5^EY06473

(Dorsett et al., 2005)

Cytology

Description

Excision of P{EPgy2}pds5^EY06473 using Δ2-3 results in a deleted sequence 5' to the insertion site extending upstream of the pds5 transcription start site.

(Dorsett et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage

(Dorsett et al., 2005)

lethal - all die before end of larval stage (with pds5^e6)

(Gause et al., 2010, Dorsett et al., 2005)

some die during third instar larval stage

(Dorsett et al., 2005)

some die during third instar larval stage (with pds5^e6)

(Gause et al., 2010, Dorsett et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous pds5^e3 and heterozygous pds5^e3/pds5^e6 mutants are lethal in late third instar to early pupal stages of development. Late third instar larvae of both mutants display small or missing imaginal discs, and the larval brains are approximately half the volume of wild-type, consistent with a mitotic defect.

The vast majority of metaphases in pds5^e3 are abnormal. Nearly all display aneuploidy (approximately 100%), and most display precocious sister chromatid separation (65%). By contrast approximately 15.4% of the pds5^e3/+ heterozygote metaphases show aneuploidy and 12.8% exhibit sister chromatid separation, similar to the frequencies observed with wild-type neuroblasts.

The salivary glands of homozygous pds5^e3 mutants are reduced. In size-matched third instars, the pds5^e3 mutant chromosomes are thicker and less extended than in wild-type.

(Dorsett et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pds5[+]/pds5^e3 is an enhancer of visible phenotype of Scer\GAL4^da.G32, wapl^{AG.UAS.Tag:HA}

(Cunningham et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

pds5^e3 is an enhancer of sex comb | ectopic phenotype of Pc¹

(Schaaf et al., 2013)

pds5^e3 is an enhancer of sex comb | ectopic phenotype of Pc⁶

(Schaaf et al., 2013)

pds5[+]/pds5^e3 is an enhancer of sex comb | ectopic phenotype of Scer\GAL4^da.G32, wapl^{AG.UAS.Tag:HA}

(Cunningham et al., 2012)

pds5[+]/pds5^e3 is an enhancer of wing margin phenotype of ct^K

(Dorsett et al., 2005)

NOT Enhancer of

Statement

Reference

pds5^e3 is a non-enhancer of sex comb | ectopic phenotype of Psc¹

(Schaaf et al., 2013)

pds5^e3 is a non-enhancer of sex comb | ectopic phenotype of Sce¹

(Schaaf et al., 2013)

pds5^e3 is a non-enhancer of sex comb | ectopic phenotype of ph-d⁴⁰¹

(Schaaf et al., 2013)

pds5^e3 is a non-enhancer of abdominal segment | adult stage phenotype of ph-d⁴⁰¹

(Schaaf et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of pds5^e3/+ further darkens the eye colour of flies homozygous for P{w^en.8}10C in a wapl^AG/+ w[-] background.

pds5^e3/+ enhances the extra sex comb phenotype caused by expression of wapl^{AG.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^da.G32.

(Cunningham et al., 2012)

ct^K mutants heterozygous for pds5^e3 exhibit a slightly increased number of wing margin nicks compared to ct^K heterozygotes, although this is not significantly different from that seen in ct^K single mutants.

(Dorsett et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pds5^e6/pds5^e3 is rescued by pds5^Chi.EGFP

(Gause et al., 2010)

Comments

pds5^{Chi.T:Avic\GFP-EGFP} rescues pds5^e3/pds5^e6 animals to adulthood.

(Gause et al., 2010)

Images (0)