FlyBase Allele Report: Dmel\Zfrp8[SM206]

General Information

Symbol

Dmel\Zfrp8^SM206

Species

D. melanogaster

Name

FlyBase ID

FBal0197282

Feature type

allele

Associated gene

Dmel\Zfrp8

Associated Insertion(s)

Carried in Construct

Also Known As

Df(2R)SM206, Zfpr8^null

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Minakhina et al., 2003)

Progenitor genotype

P{lacW}Zfrp8^k13705

(Minakhina et al., 2003)

Cytology

Description

Imprecise excision of the P{lacW}Zfrp8^k13705 insertion, resulting in a deletion that removes some Zfrp8 sequences.

(Minakhina et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Ovarioles of Zfrp8^SM206/+ mutant females do not display any morphological defects.

Zfrp8^SM206/Zfrp8^SM206 mutants display nearly complete lethality.

(Tan et al., 2016)

Persistent homozygous follicle stem cell clones are observed in only approximately 20% of ovarioles 10 days after clone induction (wild-type persistent clones are seen in nearly 60% of ovarioles at this time), and 20 days after clone induction, only 2% of ovarioles contain homozygous follicle stem cell clones (in contrast to 38% of ovarioles containing wild-type control clones). The homozygous persistent clones are smaller and encompass fewer follicle cells compared to wild-type control clones. The frequency of homozygous escort cell clones is normal 10 and 20 days after clone induction. The number and morphology of transient homozygous clones is similar to wild-type controls at 2 and 5 days after clone induction.

The frequency of homozygous germline clones is similar to that of control clones at 10 and 20 days after clone induction, but the mutant cysts show a delay in growth and development that increases in severity with time. Egg chambers older than stage 8 are not detected in either mosaic ovarioles or in ovarioles containing only mutant germ cells. Small mutant cysts are often seen posterior to more mature wild-type cysts. Cysts mostly appear to reach stage 4 or 5 in ovarioles containing only mutant germ cells. 60% of mutant germline stem cell clones contain morphologically abnormal spectrosomes: there is often no spectrosome visible on the cap-side, and only a short dumbbell-shaped spectrosome connecting the germline stem cell to the daughter cystoblast is seen.

(Minakhina et al., 2014)

Wild-type and homozygous clones in the posterior signaling center (PSC) of the lymph gland are obtained with similar frequency after induction between 6-18 hours of development in embryos. However, the types of non-PSC clones in the lymph gland that are recovered are different for wild type and homozygous clones. Homozygous type 1 clones (clones that encompass 10-30% of all the lymph gland and form cohesive clusters, and have the characteristics expected if they have been induced in the hematopoietic stem cells) are not recovered (in contrast to wild type). The percentage of homozygous type 2 clones (clones that encompass 4-5% of lymph gland hemocytes subdivided into clusters of 20 to 100 cells each) that are recovered is reduced compared to type 2 wild-type clones. The percentage of homozygous type 3 and 4 clones (small clones of dispersed cells that arise from cells with no self-renewal properties) that are recovered is increased compared to type 3 or 4 wild-type clones, especially when induced in young embryos. The percentage of mosaic animals with no lymph gland clones is double that of wild type when homozygous clones are induced. Despite the shift in proportions of the different types of clones produced, the phenotypes of the homozygous 2, 3 and 4 clones are indistinguishable from that of wild type. There is no hemocyte or lamellocyte overproliferation within homozygous lymph gland clones. The pluripotency of homozygous prohemocytes is the same as that of wild-type cells.

(Minakhina and Steward, 2010)

Homozygotes show a severe growth and developmental delay and most animals die as larvae. Few animals survive until pupariation and those that do are smaller than their heterozygous siblings. Approximately 2% of homozygous escapers survive until adulthood, but they show poor viability and female fertility.

Homozygous larvae show massive overgrowth of the lymph glands, with the glands growing up to 70 times normal size. The relative proportions of the primary and secondary lobes are preserved in the mutant glands. The number of plasmatocytes and crystal cells is increased compared to wild type, but their proportion in the mutant lymph gland is similar to that in wild type. The number of lamellocytes is drastically increased in the mutant lymph gland; the proportion of lamellocytes in the mutant gland varies from 5-60% (compared to less than 0.5% in wild type) and they are not restricted to the cortical zone of the primary lobes, being found in the presumptive medullary zone and in secondary lobes in the mutant larvae. The size and appearance of the posterior signaling centre is similar in mutant and wild-type late third instar lymph glands. The number of circulating hemocytes in mutant third instar larvae is increased about six times compared to wild type.

Homozygous late third larval instar lymph glands have a greater proportion of cells in S phase compared to controls and the mitotic index is increased. The increase in mitotic cells is concentrated in the secondary lymph gland lobes.

The lymph glands of heterozygous larvae are approximately twice the size of the average wild-type gland.

(Minakhina et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Zfrp8[+]/Zfrp8^SM206 is a suppressor | partially of abnormal dorsal/ventral axis specification | maternal effect phenotype of Scer\GAL4^{VP16.nanos.UTR}, tral^GL00680

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor of abnormal cell number | adult stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor of abnormal cell number | adult stage phenotype of Fmr1^Δ50M/Fmr1³

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor | partially of female semi-sterile phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor | partially of female semi-sterile phenotype of Fmr1^Δ50M/Fmr1³

(Tan et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Zfrp8^SM206 has embryonic/larval lymph gland phenotype, suppressible by pnr^MD237/pnr[+]

(Minakhina et al., 2007)

Zfrp8^SM206 has embryonic/larval lymph gland phenotype, suppressible by pnr[+]/pnr^VX6

(Minakhina et al., 2007)

Enhancer of

Statement

Reference

Zfrp8[+]/Zfrp8^SM206 is an enhancer of ovary | adult stage phenotype of RpS2^P/RpS2^PRW1

(Minakhina et al., 2016)

Suppressor of

Statement

Reference

Zfrp8[+]/Zfrp8^SM206 is a suppressor of ovary | adult stage phenotype of Fmr1^Δ50M/Fmr1³

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor of egg chamber | adult stage phenotype of Fmr1^Δ50M/Fmr1³

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor of nurse cell | adult stage phenotype of Fmr1^Δ50M/Fmr1³

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor of germarium | adult stage phenotype of Fmr1^Δ50M/Fmr1³

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor | partially of egg chorion | maternal effect phenotype of Scer\GAL4^{VP16.nanos.UTR}, tral^GL00680

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor | partially of dorsal appendage | maternal effect phenotype of Scer\GAL4^{VP16.nanos.UTR}, tral^GL00680

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor of ovary | adult stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor | partially of egg chamber | adult stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor of nurse cell | adult stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Tan et al., 2016)

Zfrp8[+]/Zfrp8^SM206 is a suppressor | partially of germarium | adult stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Tan et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Zfrp8^SM206/+ enhances the ovary phenotypes of RpS2^P/RpS2^PRW1 mutants.

(Minakhina et al., 2016)

Zfrp8^SM206/+ partially rescues the ventralized chorion phenotypes of eggs laid by females expressing tral^GL00680 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

Zfrp8^SM206/+ partially restores fertility and suppresses the majority of ovary defects in Fmr1^Δ50M/Df(3R)Exel6265 mutants, restoring cell division in the germline, normal oogenesis, and normal egg chamber morphology and separation, although the first egg chamber often still appears fused to the germarium.

Zfrp8^SM206/+ partially restores fertility and restores ovary morphology to normal in Fmr1^Δ50M/Fmr1³ mutants.

(Tan et al., 2016)

The slight increase in lymph gland size seen in Zfrp8^SM206/+ larvae is dominantly suppressed by pnr^MD237 or pnr^VX6. The increase in lymph gland size seen in homozygous Zfrp8^SM206 larvae is significantly dominantly suppressed by pnr^MD237.

(Minakhina et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Zfrp8^SM206 is rescued by Zfrp8^UAS.Tag:HA/Scer\GAL4^hs.PB

(Minakhina et al., 2007)

Partially rescued by

Zfrp8^SM206 is partially rescued by Zfrp8^UAS.GFP/Scer\GAL4^hs.PB

(Tan et al., 2016)

Zfrp8^SM206 is partially rescued by Zfrp8^{UAS.GFP,Tag:NLS(SV40-largeT)}/Scer\GAL4^hs.PB