FlyBase Allele Report: Dmel\koi[80]

General Information

Symbol

Dmel\koi⁸⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0197602

Feature type

allele

Associated gene

Dmel\koi

Associated Insertion(s)

TI{TI}koi⁸⁰

Carried in Construct

Also Known As

koi^HRKO80, koi^HRKO80.w

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Kracklauer et al., 2007)

Mutagen

recombination

(Kracklauer et al., 2007)

I-SceI

(Kracklauer et al., 2007)

FLPase

(Kracklauer et al., 2007)

ends-out gene targeting

(Kracklauer et al., 2007)

Progenitor genotype

Associated Insertion(s)

TI{TI}koi⁸⁰

Cytology

Description

The entire koi coding sequence has been replaced by w^+mW.hs marker.

(Kracklauer et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Kracklauer et al., 2007)

viable

(Kracklauer et al., 2007)

visible (with Df(2R)Exel6050)

(Kracklauer et al., 2007)

visible | recessive

(Kracklauer et al., 2007)

Phenotype Manifest In

eye

(Kracklauer et al., 2007)

eye (with Df(2R)Exel6050)

(Kracklauer et al., 2007)

myotube | third instar larval stage

(Ding et al., 2017)

nucleus & cone cell & eye disc (with Df(2R)Exel6050)

(Kracklauer et al., 2007)

nucleus & eye photoreceptor cell & eye disc

(Kracklauer et al., 2007)

nucleus & eye photoreceptor cell & eye disc (with Df(2R)Exel6050)

(Kracklauer et al., 2007)

nucleus | third instar larval stage

(Ding et al., 2017)

rhabdomere (with Df(2R)Exel6050)

(Kracklauer et al., 2007)

Detailed Description

Statement

Reference

koi⁸⁰ homozygotes exhibit both severe nuclear spacing defects (i.e. nuclear clustering) and moderate nuclear shape defects in third instar larval muscle fibers, as compared to heterozygous and wild-type controls.

(Ding et al., 2017)

koi^HRKO80.w homozygous flies and koi^HRKO80.w/Df(2R)Exel6050 trans-heterozygous flies exhibit a rough eye phenotype.

koi^HRKO80.w/Df(2R)Exel6050 mutant retinas have a mainly undisrupted hexagonal lattice of accessory cells and the trapezoids of photoreceptors are also present. However, rhabdomere morphology is aberrant.

Photoreceptor and cone cell nuclei fail to migrate apically and are positioned randomly within the apical-basal axis in koi^HRKO80.w/Df(2R)Exel6050 mutant eye discs.

Photoreceptor nuclei fail to migrate apically and are positioned randomly within the apical-basal axis in koi^HRKO80.w mutant eye discs.

(Kracklauer et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

koi⁸⁰ has visible phenotype, non-enhanceable by klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ has viable phenotype, non-enhanceable by klar^marb-CD4

(Kracklauer et al., 2007)

NOT suppressed by

Statement

Reference

koi⁸⁰ has visible phenotype, non-suppressible by klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ has viable phenotype, non-suppressible by klar^marb-CD4

(Kracklauer et al., 2007)

NOT Enhancer of

Statement

Reference

koi⁸⁰ is a non-enhancer of visible phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ is a non-enhancer of viable phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

Suppressor of

Statement

Reference

koi[+]/koi⁸⁰ is a suppressor | partially of increased cell death | somatic clone | oogenesis phenotype of kud^KO

(Ding et al., 2017)

NOT Suppressor of

Statement

Reference

koi⁸⁰ is a non-suppressor of visible phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ is a non-suppressor of viable phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

koi⁸⁰ has nucleus | third instar larval stage phenotype, enhanceable by Scer\GAL4^Mef2.247/kud^UAS.CFP

(Ding et al., 2017)

koi⁸⁰ has myotube | third instar larval stage phenotype, enhanceable by Scer\GAL4^Mef2.247/kud^UAS.CFP

(Ding et al., 2017)

NOT Enhanced by

Statement

Reference

koi⁸⁰ has eye phenotype, non-enhanceable by klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ has nucleus & eye photoreceptor cell & eye disc phenotype, non-enhanceable by klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ has interommatidial bristle phenotype, non-enhanceable by klar^marb-CD4

(Kracklauer et al., 2007)

NOT suppressed by

Statement

Reference

koi⁸⁰ has eye phenotype, non-suppressible by klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ has nucleus & eye photoreceptor cell & eye disc phenotype, non-suppressible by klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ has interommatidial bristle phenotype, non-suppressible by klar^marb-CD4

(Kracklauer et al., 2007)

Enhancer of

Statement

Reference

koi[+]/koi⁸⁰ is an enhancer of nucleus | third instar larval stage phenotype of Scer\GAL4^Mef2.247, kud^UAS.CFP

(Ding et al., 2017)

koi[+]/koi⁸⁰ is an enhancer of myotube | third instar larval stage phenotype of Scer\GAL4^Mef2.247, kud^UAS.CFP

(Ding et al., 2017)

NOT Enhancer of

Statement

Reference

koi⁸⁰ is a non-enhancer of eye phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ is a non-enhancer of nucleus & eye photoreceptor cell & eye disc phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ is a non-enhancer of interommatidial bristle phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

Suppressor of

Statement

Reference

koi[+]/koi⁸⁰ is a suppressor | partially of follicle cell | somatic clone | oogenesis phenotype of kud^KO

(Ding et al., 2017)

NOT Suppressor of

Statement

Reference

koi⁸⁰ is a non-suppressor of eye phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ is a non-suppressor of nucleus & eye photoreceptor cell & eye disc phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

koi⁸⁰ is a non-suppressor of interommatidial bristle phenotype of klar^marb-CD4

(Kracklauer et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

koi⁸⁰ heterozygosity partially suppresses the increased apoptosis observed in kud^KO homozygous follicular epithelium clones and enhances the minor/insignificant myonuclear spacing defects in third instar larval muscle fibers induced by the expression of kud^{Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^Mef2.247.

(Ding et al., 2017)

koi^HRKO80.w, klar^marb-CD4 double mutant homozygotes have a mutant phenotype identical to either single mutant alone: photoreceptor nuclei fail to migrate apically and are positioned randomly within the apical-basal axis in mutant eye discs; mutant adults are viable with rough eyes in which some bristles are missing.

(Kracklauer et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Df(2R)Exel6050 fails to complement the rough eye phenotype associated with koi^HRKO80.w.

(Kracklauer et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25105

w^*; TI{TI}koi⁸⁰

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

koi⁸⁰

(Yamamoto-Hino et al., 2024, Zheng et al., 2020, Ambrosini et al., 2019, Kracklauer et al., 2007)

koi^HRKO80.w

(Ding et al., 2017, Wesolowska et al., 2013)

koi^HRKO80

(Kracklauer et al., 2007)

koi^HRko80

(Huelsmann et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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