FB2024_03 , released June 25, 2024
Allele: Scer\GAL4odd-MC
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General Information
Symbol
Scer\GAL4odd-MC
Species
S. cerevisiae
Name
FlyBase ID
FBal0197884
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
P{GAL4}oddMC
Carried in Construct
Also Known As
odd-GAL4
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    P{GAL4}oddMC
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    P{GAL4}
    Encoded product / tool
    GAL4
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        distribution deduced from reporter (Gal4 UAS)
        Stage
        Tissue/Position (including subcellular localization)
        Reference
        embryonic stage 15
        neuroblast DPL | subset
        (Levy and Larsen, 2013)
        mushroom body neuroblast
        (Levy and Larsen, 2013)
        neuroblast BLP | subset
        (Levy and Larsen, 2013)
        second instar larval stage
        larval odd neuron of the calyx
        (Slater et al., 2015, Levy and Larsen, 2013)
        larval odd neuron of the inferior protocerebrum
        (Slater et al., 2015)
        third instar larval stage
        larval odd neuron of the inferior protocerebrum
        (Slater et al., 2015, Levy and Larsen, 2013)
        larval odd neuron of the calyx
        (Slater et al., 2015)
        prepupal stage P1 -- pharate adult stage P15
        adult odd neuron of the calyx
        (Levy and Larsen, 2013)
        adult odd neuron of the central brain
        (Levy and Larsen, 2013)
        adult odd neuron of the lobula plate
        (Levy and Larsen, 2013)
        adult stage
        lobula plate tangential neuron Hx
        (Wasserman et al., 2015)
        adult odd neuron of the calyx
        (Levy and Larsen, 2013)
        adult odd neuron of the central brain
        (Levy and Larsen, 2013)
        adult odd neuron of the lobula plate
        (Levy and Larsen, 2013)
        adult glial cell | subset
        (Levy and Larsen, 2013)
        Additional Information
        Statement
        Reference

        Positive GRASP (GFP reconstitution across synaptic partners) signals between lobula plate tangential neuron Hx labelled with ScerGAL4odd-MC and octopaminergic neurons labelled with EcollexAVP16.Tdc2 were observed in the lobula plate.

        (Wasserman et al., 2015)

        ScerGAL4odd-MC drives expression in odd positive adult neurons, as well as larval odd neurons. In the embryo, expression is seen in a subset of DPL and BLP neuroblasts, as well as the mushroom body neuroblasts - although this latter expression is not maintained later in development and it is not known which neuroblast subset odd neurons develop from. During pupal development neurons are observed to undergo pruning before regrowing projections 24-48 hours after puparium formation.

        (Levy and Larsen, 2013)
         
        Marker for
        lobula plate tangential neuron Hx
        (Wasserman et al., 2015)
        Reflects expression of
        odd
        (Slater et al., 2015, Levy and Larsen, 2013)
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Inhibition (using Hsap\KCNJ2UAS.EGFP or Ctet\TeTxLCTNT.UAS) of Scer\GAL4odd-MC neurons in larvae, in combination with a Gal80[ts] transgene to restrict expression to the larval stage, results in significantly reduced navigation towards appetitive odors, compared to controls. In the case of Hsap\KCNJ2UAS.EGFP, there is no effect on larval movement speed and no effect on navigation away from aversive odors, compared to controls; navigation towards the stronger of two similar dilutions of an appetitive odor is significantly reduced, compared to controls, but when the odor concentration is strong, they spend significantly more time closer to the source than controls; stopping, head swinging and turning frequencies are all significantly increased in the presence, but not the absence of odor, compared to controls, resulting in more convoluted locomotion tracks.

        Activation (using Bhal\NaChBacUAS.cNa, or using TrpA1UAS.cUa at 30[o]C) of Scer\GAL4odd-MC neurons in larvae, in combination with a Gal80[ts] transgene to restrict expression to the larval stage, results in significantly increased navigation towards appetitive odors, compared to controls. In the case of Bhal\NaChBacUAS.cNa, there is no effect on larval movement speed, compared to controls; navigation towards the stronger of two similar dilutions of an appetitive odor is significantly increased, compared to controls, but when the odor concentration is strong, they spend significantly more time further from the source than controls; stopping and head swinging, but not turning, frequencies are significantly decreased in the presence, but not the absence, of odor, compared to controls.

        (Slater et al., 2015)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer

        G. Morata

        (Bras-Pereira et al., 2006)

        M. Calleja

        (Bras-Pereira et al., 2006)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        Scer\GAL4odd-MC
        Name Synonyms
        Secondary FlyBase IDs
          References (5)