FB2024_03 , released June 25, 2024
Allele: Dmel\Mnn1UAS.cPa
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General Information
Symbol
Dmel\Mnn1UAS.cPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Papaconstantinou
FlyBase ID
FBal0198069
Feature type
allele
Associated gene
Dmel\Mnn1
Associated Insertion(s)
Carried in Construct
P{UAS-Mnn1.P}
Also Known As
UAS-Mnn1
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Papaconstantinou et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-Mnn1.P}
Cytology
Description

UASt regulatory sequences drive expression of 4411 nucleotides of genomic DNA corresponding to exons 2 to 5 of the Mnn1 gene.

(Papaconstantinou et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mnn1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Mnn1Scer\UAS.cPa following a 1 hour heat shock at 37oC, under the control of Scer\GAL4hs.PB, Scer\GAL4nos.PG, Scer\GAL4arm.PS or Scer\GAL4αTub84B.PL show embryonic lethality. The lethality follows a dramatic increase in cell death. However, expression of Mnn1Scer\UAS.cPa under the control of Scer\GAL4nos.PG, Scer\GAL4αTub84B.PL or Scer\GAL4arm.PS at 25oC does not result in this lethality.

      Expression of Mnn1Scer\UAS.cPa under the control of Scer\GAL4nos.PG following 2 hours in hypoxic conditions (5% O2) results in embryonic lethality, while expression at 21% O2 results in similar survival levels to wild-type flies.

      Expression of Mnn1Scer\UAS.cPa under the control of Scer\GAL4αTub84B.PL in a hyper-osmolarity environment (0.2M NaCl) results in leads to lethality, while flies of the same genotype raised in a 0M NaCl environment show similar survival levels to wild-type flies.

      Exposure of adult flies that express Mnn1Scer\UAS.cPa under the control of Scer\GAL4αTub84B.PL to 5mM paraquat results in results in decreased viability compared to wild-type flies exposed to this chemical.

      (Papaconstantinou et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Mnn1Scer\UAS.cPa
      Mnn1UAS.cPa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Papaconstantinou
      Secondary FlyBase IDs
        References (2)