FlyBase Allele Report: Dmel\Mnn1[RNAi.UAS]

General Information

Symbol

Dmel\Mnn1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0198070

Feature type

allele

Associated gene

Dmel\Mnn1

Associated Insertion(s)

Carried in Construct

P{UAS-Mnn1.RNAi}

Also Known As

UAS-Mnn1-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Papaconstantinou et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Papaconstantinou et al., 2005)

Mutagen

in vitro construct

(Papaconstantinou et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Mnn1.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of exon 2 of the Mnn1 gene, followed by intron 2, followed by exon 2 in reverse order.

(Papaconstantinou et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mnn1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response, with Scer\GAL4^nanos.PG

(Papaconstantinou et al., 2005)

abnormal stress response, with Scer\GAL4^αTub84B.PL

(Papaconstantinou et al., 2005)

lethal | conditional | embryonic stage, with Scer\GAL4^nanos.PG

(Papaconstantinou et al., 2005)

lethal | embryonic stage | heat sensitive, with Scer\GAL4^nanos.PG

(Papaconstantinou et al., 2005)

lethal - all die during embryonic stage | heat sensitive, with Scer\GAL4^arm.PS

(Papaconstantinou et al., 2005)

lethal - all die during embryonic stage | heat sensitive, with Scer\GAL4^hs.PB

(Papaconstantinou et al., 2005)

lethal - all die during embryonic stage | heat sensitive, with Scer\GAL4^αTub84B.PL

(Papaconstantinou et al., 2005)

some die during embryonic stage | heat sensitive, with Scer\GAL4^arm.PS

(Papaconstantinou et al., 2005)

some die during embryonic stage | heat sensitive, with Scer\GAL4^hs.PB

(Papaconstantinou et al., 2005)

some die during embryonic stage | heat sensitive, with Scer\GAL4^αTub84B.PL

(Papaconstantinou et al., 2005)

viable, with Scer\GAL4^GMR.PF

(Papaconstantinou et al., 2005)

viable, with Scer\GAL4^LE

(Papaconstantinou et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mnn1^{dsRNA.Scer\UAS} following a 1 hour heat shock at 37^oC, under the control of Scer\GAL4^hs.PB, Scer\GAL4^nos.PG, Scer\GAL4^arm.PS or Scer\GAL4^αTub84B.PL show embryonic lethality. The lethality follows a dramatic increase in cell death. However, expression of Mnn1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^nos.PG, Scer\GAL4^αTub84B.PL or Scer\GAL4^arm.PS at 25^oC does not result in this lethality.

Expression of Mnn1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^nos.PG following 2 hours in hypoxic conditions (5% O₂) results in embryonic lethality, while expression at 21% O₂ results in similar survival levels to wild-type flies.

Expression of Mnn1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL in a hyper-osmolarity environment (0.2M NaCl) results in leads to lethality, while flies of the same genotype raised in a 0M NaCl environment show similar survival levels to wild-type flies.

Exposure of adult flies that express Mnn1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL to 5mM paraquat results in results in decreased viability compared to wild-type flies exposed to this chemical. Scer\GAL4^αTub84B.PL>Mnn1^{dsRNA.Scer\UAS} expression does not alter the development of the trachea in control conditions but in a low oxygen environment, the induction of lateral branching seen in wild-type larvae is inhibited in Scer\GAL4^αTub84B.PL>Mnn1^{dsRNA.Scer\UAS} larvae.

(Papaconstantinou et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-Mnn1.RNAi} construct should produce Mnn1 dsRNA, resulting in dsRNA interference (RNAi) of the Mnn1 gene.

(Papaconstantinou et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mnn1^RNAi.UAS

Mnn1^{dsRNA.Scer\UAS}

Mnn1^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close